Sorsogon National High School

Sorsogon City

Youth for Environment in Schools Organization

Active Association of Youth Science Clubs

Objective
General
Provide the scientific framework of the
OL Trap Program of Dengue vector
control

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

Objective
Specific

QUITO OL-TRAP

February 5, 2015

Explain the concept of vector control

Explain dengue vector control
Explain Integrated Vector Control
(IVC) and Integrated Vector
Management (IVM)
Discuss the components of IVC from
vector biology to control measures

DOST School-based O/L Trap Program

Objective
Specific

QUITO OL-TRAP

February 5, 2015

Describe vector surveillance as it

applies to dengue OL Trap in a
classroom setting
Answer the fourteen questions
enumerated in the situationer

DOST School-based O/L Trap Program

Questions
How many ovitraps/OL Traps are
needed to be installed in a room?
Where should the traps be installed?
When should the traps be installed?
How frequent should the traps be
installed?

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

Questions

QUITO OL-TRAP

What is the procedure to be

followed in installing the traps? In
servicing the traps? What is the
meaning of servicing the traps?
Who will install and service the
traps?

February 5, 2015

DOST School-based O/L Trap Program

Questions
Who will monitor and check the
traps?
Monitor

QUITO OL-TRAP

Empty tumbler
Missing tumbler/paddle

Check
If positive/negative?

Is there a form to be filled-out?

February 5, 2015

DOST School-based O/L Trap Program

Questions
What index needs to be obtained?
What formula is to be used?
What is the meaning of the index
obtained?
Who will record and compute for the
index?

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

Questions
To whom should the data be
submitted to? Why?
For how long will this ovitrapping be
done?

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

Whats inside?
Vector
Surveillance
Prevention and Control

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

10

Surveillance

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

11

Vector Surveillance
Collection and interpretation of data
which can be used to guide mosquito
vector control operations
Establish baseline
Presence or absence of vector

QUITO OL-TRAP

Species?
Density?
Changes?
February 5, 2015

DOST School-based O/L Trap Program

12

Dengue Surveillance Tool

Classical OVITRAP/LARVITRAP (black
tumbler, paddle, water)
Modified OVITRAP is the OLTRAP
(black container, paddle,
water
and organic
larvicide)
2 adults (females)
emerging per day
(Malaysian experience)

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

13

Difference between tools

Ovitrap

QUITO OL-TRAP

February 5, 2015

OL Trap

Eggs on the paddle

- Yes

- Yes

Live larva seen

- Yes

- No

Action

- Eggs trapped

- Eggs trapped

With organic larvicide

Value-added

Risk

- with white marks

- same

Benefit

- Breeds Aedes, if
uncollected (set and
collect)

- Breeds Aedes, if
uncollected after 7
days (set and collect)

Index (CTDI)

- Ovitrap index : 10%

- OLTrap: 10%

None
None

DOST School-based O/L Trap Program

- Yes
- Additional attractant
(kills larva)

14

Ovitrap Index
Total Number of positive traps X
Total Number of traps planted
100%

QUITO OL-TRAP

If OI is >10%, the place is

vulnerable to dengue transmission.

February 5, 2015

DOST School-based O/L Trap Program

15

Critical Threshold Density

Index (CTDI)
Actual target for the degree of
vector suppression
Predictive value for the prevention of
dengue transmission

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

16

Remember:
A positive trap means there is at
least one mosquito laying its eggs in
the sampling site (surveillance site)
Aim is eliminate this one mosquito
laying its eggs

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

17

What is DOST Mosquito Ovi/Larvicidal

(OL) Trap System?
Is a system used to control the population of the
dengue carrying Aedes mosquitoes.
It has three important parts: a black container, a
small strip of lawanit measuring 1 x 6.5 for
mosquitoes to lay their eggs on, and pellets as
larvicide.

QUITO OL-TRAP

Tumbler

The OL-Traps ovicidal and larvicidal effect

prevent the next generation of mosquitoes from
reaching adulthood, thus curbing the Aedes
mosquito population.

Paddle

February 5, 2015

DOST School-based O/L Trap Program

Pellets

18

HOW THE OL-TRAP WORKS?

The idea is to attract mosquitoes particularly the

Aedes aegypti and albopictus (dengue carrier) to lay
their eggs on the lawanit paddle.

The trap creates optimum conditions for this species

to lay its eggs: stagnant, clean, clear water, dark
area and damp rough surface.

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

19

The eggs will either be trapped in the lawanit paddle

or find its way into organic solution, where they are
killed before transforming into adults.

Eggs

QUITO OL-TRAP

Larvae

February 5, 2015

DOST School-based O/L Trap Program

20

Distribution of OL Trap

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

21

CLASSROO
M
1 OL Trap indoor
and
1 OL Trapoutdoor

CLASSROO
M
1 OL Trap indoor
and
1 OL Trapoutdoor

CLASSROO
M
1 OL Trap indoor
and
1 OL Trap-outdoor

CLASSROO
M
1 OL Trap indoor
and
1 OL Trap-outdoor

SCHOOL

CLASSROO
M
1 OL Trap indoor
and
1 OL Trapoutdoor

1 OL Trap indoor
1 Tumbler (Black
Container)
1 sachet of pellet per
week
1 pc paddle per month

1 OL Trap outdoor

CLASSROO
M

1 Tumbler (Black
Container)
1 sachet of pellet per
week
1 pc paddle per
month

Servicing the OL Trap

OVITRAPS are installed and serviced
every seven days in locations where
transmission of dengue is probable

QUITO OL-TRAP

nursery schools
pre-schools
elementary schools
Hospitals
community with confirmed dengue
cases
Ovitrap Index = > 10%

February 5, 2015

DOST School-based O/L Trap Program

24

Before OL Trap Installation

QUITO OL-TRAP

Clean-up all possible breeding sites/containers found

inside/outside the classrooms DO SEARCH and
DESTROY

Artificial containers
(man-made)
1. Flower vases and pot plates
2. Pails, water-storage jars, basins
3. Discarded receptacles
4. Choked roof gutters
5. Gully Traps
6. Unused toilet bowls and cisterns
7. Aircon Tray and dish rack tray
8. Concrete drains

February 5, 2015

Natural containers
1. Tree Holes, bamboo stumps
2. Leaf axils, fallen leaves
3. Ground Depressions

DOST School-based O/L Trap Program

25

Where to set up the DOST OL-Trap?

A. Indoor:

QUITO OL-TRAP

Under the sink

Corner of the room
Under the cabinet
Under the bed
Inside the comfort room

February 5, 2015

B. Outdoor (covered area):

Corner of the classroom
Near vegetation
Piles of woods/hollow
blocks
Garden

DOST School-based O/L Trap Program

26

Setting the OL Trap

Step 1

QUITO OL-TRAP

Fill the plastic tumbler with tap

water up to the lined mark just
above the DOST logo
(~250 ml)

February 5, 2015

DOST School-based O/L Trap Program

27

Setting the OL Trap

Step 2

QUITO OL-TRAP

Pour one pack OL pellets

into the plastic cup
Stir thoroughly to ensure
pellets are dissolved

February 5, 2015

DOST School-based O/L Trap Program

28

Setting the OL Trap

Step 3
Place the lawanit stick in the
solution with the rough
surface facing up

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

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Setting the OL Trap

Step 4
Place 1 cup each inside
and outside the classroom
in a low-lying and dark area

QUITO OL-TRAP

If outside, protect from direct

sunlight and rainfall
Take care not to spill the
contents
Keep out of reach of children
and animals

February 5, 2015

DOST School-based O/L Trap Program

30

Setting the OL Trap

Step 5

Collect trap and note for

the following on the seventh (one
week after installation (every
Monday) day

QUITO OL-TRAP

Eggs on the paddle

Live larva/pupa
The presence of even one
larvae indicates a positive trap
Record in the OL Trap
Monitoring Sheet (3 copies)

February 5, 2015

DOST School-based O/L Trap Program

31

Setting the OL Trap

Step 6
Repeat steps 1-5 every 7 days

QUITO OL-TRAP

Set and collect the traps every Monday

Finalize report every Tuesday
Note: If collection period falls on a holiday,
please collect traps a day before

February 5, 2015

DOST School-based O/L Trap Program

32

Cleaning the Trap

Vigorously brush-off into
the solution eggs stuck
into the lawanit creases
Kill the larva/eggs by
pouring hot water into
the black tumbler
Dispose contents
properly by pouring
contents on dry ground

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

33

Identifying a POSITIVE/NEGATIVE Trap

QUITO OL-TRAP

Presence (+)/ absence (-)

of eggs in the paddle

Presence (+)/ absence (-) of larvae

in the container/tumbler with water

February 5, 2015

DOST School-based O/L Trap Program

34

An OL Trap is positive or negative if;

QUITO OL-TRAP

Paddle

Tumbler

Positive
(+)

Positive
(+)

POSITIVE (+)

Positive
(+)

Negative
(-)

POSITIVE (+)

Negative
(-)

Positive
(+)

POSITIVE (+)

Negative
(-)

Negative
(-)

NEGATIVE (-)

February 5, 2015

RESULT

DOST School-based O/L Trap Program

35

OL Trap Monitoring
Sheet

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

36

Prevention / Control

QUITO OL-TRAP

February 5, 2015

DOST School-based O/L Trap Program

37