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Bacterial genetics
DNA molecule structure, base
arrangements: double helix strands;
composed of many neucleotides; A & G
are purine; C & T are pyrimidine
Plasmids
1. Structure : small circular DNA molecule
that can exist independently of host
chromosome. They have their own
replication origins, carry fewer genes
compared to DNA.
2. types of plasmids and importance : 6
different types.
Mutation
1. (a)meaning : an inherited change in
the base sequence of the genome of
an organism
2. (b)replica plating : a technique used
to detect auxotrophic* mutants.
3. (c)types of mutation
(i)molecular types of mutations- base pair(point
mutation): affects only one base pair in a given
location. Transition : replacement of a pyrimidine on
one strand by a different pyrimidine/ replacement of
a purine on one strand by a different purine.
Transversion : purine replace pyrimidine vice versa.
silent mutation : do not alter phenotype of organism
and go undetected. More than one codon for a given
amino acid. ‘wobble” – variability in the 3rd base
position.
Missense mutation : involves a single base
substitution in the DNA that changes a codon for
one a.a. into a codon for another. Expressed at
the level of protein structure
however at the level of protein function, the effect may
range from complete loss of activity to no change at all.
Many proteins are still functional after substitution
of a single a.a. but depends on the type and
location of the a.a.
Eg. 1. – replacement of a nonpolar with
polar a.a.- drastic change
Eg 2. – replacement of a critical a.a. at the
active site will destroy its activity
Eg. 3 – replacement of one polar with
another polar a.a., may have little/ no
effect.
Missense mutation
provides evolution
often non-lethal/remain in the pool.
(ii)frameshifts mutation: causes a change in he 3 base