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Biochemistry
Introduction to Biochemistry
Part 1
What is Biochemistry?
Bio-
means life
What is Biochemistry?
The
Researches in Biochemistry
Researches about photosynthesis, respiration, nucleic acids, metabolism
and others can be considered as the beginning of Biochemistry
Louis
Researches in Biochemistry
Gustav
Researches in Biochemistry
H.
study:
Structure and properties of substance that constitute
the framework of cells and tissues; as source of
energy/ leaving the cells as waste products
Helps in the diagnosis of diseases ( arthritis, diabetes,
etc)
Discovery of new drugs
Application in laboratory
Improvement of agriculture
Nutrition
Beauty and wellness
Living
Carbon (C)
most
most predominant
predominant and
and versatile
versatile element
element of
of life
life
possesses
possesses unique
unique property
property to
to form
form infinite
infinite number
number of
of
compounds
compounds
ability
ability of
of carbon
carbon to
to form
form stable
stable covalent
covalent bonds
bonds and
and C-C
C-C
chains
chains of
of unlimited
unlimited length
length
estimated
estimated that
that about
about 90%
90% of
of compounds
compounds found
found in
in living
living
system
system invariably
invariably contain
contain carbon
carbon
Chemical Composition
of Man
Water
Water is
is
the
the solvent
solvent of
of life
life and
and
contributes
contributes to
to more
more
than
than
60%
60% of
of the
the weight
weight
protein
protein
(mostly
(mostly in
in muscle)
muscle)
lipid
lipid (mostly
(mostly in
in
adipose
adipose
Tissue
Tissue
carbohydrate
carbohydrate
content
content is
is rather
rather
low
low
which
which is
is in
in the
the form
form of
of
glycogen.
glycogen.
The Biomolecules of
Cells
The Cell
A single
adult body consists of
an unbelievably large
number of cells...
between 60 and
100 trillion.
The cell is the smallest
unit inside our bodies
that can be
classified as living.
Cell
Cell is
is the
the
structural
structural and
and
functional
functional unit
unit
of
of life.
life.
It
may
It may be
be also
also
regarded
regarded as
as the
the
basic
basic unit
unit
of
of biological
biological
activity
activity
Concept
Concept of
of cell
cell
originated
originated from
from
the
contributions
the contributions
of
of Schleiden
Schleiden and
and
Schwann
Schwann (1838)
(1838)
It
It was
was only
only
after
after 1940,
1940, the
the
complexities
complexities of
of
cell
cell structure
structure
were
were exposed
exposed
Prokaryotes
Eukaryotes
Comparison
Animal Cell
Plant Cell
Nucleus
Largest cellular organelle
surrounded by double membrane
nuclear envelope
Contains DNA
repository of
genetic info
Associated with basic protein called histone (1:1
ratio)
Form
nucleosomes
an
assembly
constitutes
chromatin fibres of
chromosomes( Greek chroma colour; soma
body)
A single human chromosome is composed of about a
million nucleosomes
Number of chromosomes is a characteristic feature of the
species
Humans have 46 chromosomesc, compactly
packed in the nucleus
Nucleus
Contains nucleolus
rich in RNA (ribosomal RNA)
enters the cytosol through nuclear pore
ground material of the nucleus is often referred to as
nucleoplasm
rich in enzymes such as DNA
polymerases and RNA polymerase
visible in the nucleus, frequently near the nuclear
membrane, is chromatin, an aggregate of DNA and
protein.
Mitochondria
(Greek'. mitos - thread; chondros granule)
center for the cellular
respiration and energy
metabolism
like the nucleus, has a double
membrane
outer membrane has a fairly smooth
surface, but the inner membrane
exhibits many folds called cristae
(Latin crests)
space within the inner membrane is called the matrix
or mitosol ( contains a circular double stranded DNA
(mtDNA), RNA and ribosome)
Mitochondria
Endoplasmic
Reticulum (ER)
attached to the cell membrane and
to the nuclear membrane
two forms, rough and smooth
o rough endoplasmic
reticulum is studded with
ribosomes bound to the
membrane
sites of protein synthesis in all organisms
o smooth endoplasmic reticulum does not have
ribosomes bound to it
is involved in the
synthesis of lipids (triaglycerols, phospholipids,
sterols)and metabolism of drugs, besides supplying
Ca for the cellular functions
Golgi Apparatus
Golgi Apparatus
Lysosomes
Lysosomes
Peroxisomes
Peroxisomes
Cytosol &
Cytoskeleton
In summary..
Inside Cells
Protein synthesis
of cellular structure
Digestion
Muscle
creation
Protection from viral, fungal, and parasitic
infections
Proteins are continuously manufactured by
every cell in our body.
Protein Synthesis
Metabolism
Once proteins are created, they do important
jobs:
catalyzing the breakdown of foods or
medicines that enter the body into something
useful
metabolism
Play the central role in driving metabolism
Metabolism
Metabolism
Energy Production
o To maintain essential
cellular and
metabolic processes,
cells must produce a
constant supply of
ATP
o To do this, they
require sugar content
(that is, saccharides
or carbohydrates)
and oxygen
o ATP is created by
mitochondria and
proteins found in the
cytosol
Remember:
ATP is the common currency
of
energy thats used by proteins
to keep us alive.
Photosynthesis
Photosynthesis is a reaction that uses sunlight and carbon
dioxide to synthesize carbohydrates (saccharides)..
Carbohydrates were required to create ATP, and oxygen is
created as a by-product of photosynthesis..
Carbohydrates and oxygen are required to create ATP, which is
essential for our bodies... and both of those things are produced
by photosynthesis!
Photosynthesis
Photosynthesis occurs
occurs
in
in chloroplasts,
chloroplasts, which
which
are
are special
special organelles
organelles
found
found in
in plant
plant cells
cells
Protein synthesis is
carried out by
ribosomes,
which float in the
cytoplasm
or are stuck to the
endoplasmic
reticulum.
Amino acids
are combined by
chemical
reactions
to form
proteins...
Protein
So protein
synthesis is in
fact...
biochemistry!
Biochemistry of Metabolism
For example,
gluconeogenesis,
which is performed by liver or
kidney cells, is a chemical
reaction that changes pyruvic
acid into a saccharide called
glucose.
Lipogenesis is a chemical
reaction that changes
saccharides into fat when too
many saccharides are absorbed
into your body.
Biochemistry of Energy
Production
To produce energy, glucose
is first broken down into
pyruvic
acid in cytosol.
Energy production
is also a kind of
metabolism.
The
reverse version of
gluconeogenesis,
called glycolysis
is all about
breaking down
carbohydrates!
Biochemistry of Energy
Production
Biochemistry of
Photosynthesis
A
A complex
complex
chemical
chemical
reaction
reaction occurs
occurs
when
when light
light strikes
strikes
chloroplasts
chloroplasts in
in a
a
plant's
plant's cells.
cells.
Summary
The processes
that occur in
our
cells are
chemical
reactions!
Tight regulation of
these processes
ensures that
everything occurs
in the proper order,
which is essential
to cell life.
ical
m
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Closely
The
When
Carbon Cycle
Carbon is one of the most
important chemical elements
to living creatures Carbon is at the center of amino
acids, which are the building
blocks of proteins.
Its also the element that
creates the framework of
carbohydrates and lipids, and
its a vital part of DNA.
Carbon Cycle
When carbon leaves an
organisms body, it can bond with
two oxygen atoms to become
carbon dioxide (CO2) or bond
with four hydrogen atoms to
become methane (CH4).
it can accumulate deep underground and, over
long periods of time, it will become crude oil,
coal, and, in some cases, even diamonds.
Photophosphorylation
The
The two
two most
most
important
important reactions
reactions
in
in photosynthesis
photosynthesis are
are
photophosphorylatio
photophosphorylatio
n
n
and
and carbon
carbon dioxide
dioxide
fixation
fixation
Photophosphorylation
Different shapes is a complex
These are the part of the
consisting of several proteins
electron transport chain.
gathered together.
When sunlight
strikes
chlorophyll,
the molecule is
"excited" by the
light energy
Photophosphorylation
Since the electrons are passed along to another complex, it's
called an "electron transport chain.
Photophosphorylation
Photophosphorylation
Photophosphorylation
So ATP is synthesized
by
the flow of electrons
triggered by sunlight...
Light energy turns into
Chemical energy!
References
Biochemistry,
Masaharu
H,Stephen