Development of Laboratory

based portable spectrometer

Professor : Byoung-Kwan Cho

Lalit Kandpal |Kwon kyung-do |Min Hyun jung |Dewi Kusumaningrum |Collins

Contents

1
2
3
4
5

Spectrometer developemnt
Sample spectra collection
Wavelength calibration
Data analysis

Results

System components

Accessories for spectrometer development

System hardware

crossed czerny-turner spectrograph arrangement

Laboratory developed spectrometer

General principle of spectrometer

Sample Preparation
3%

5%

7%

10%

15%

17%

20%

22% 25%

13%

Orange juice

10 samples each concentration

Total :100 samples
For the development of PLS model large amount of samples require, therefore in this study we used 100 samples.
Where, Calibration 70 & validation 30 samples.

Data acquisition software

Hardware calibration

SPLICCO

Data acquisition

CSharp

Spectra collection procedure

1

Dark

Reference

Sample

Wavelength calibration with LED light

LED spectra from spectrometer

HSI spectra of LED light

Waveband
(Hyper)

Pixel
(spectromet
er)

459.4

448

531.1

626

550.1

660

736.4

1064

893.9

1449

Correlation between waveband (hyper) & pixel (spectrometer)

Raw spectra of orange juice

600 nm: due to the yellow color of juice samples
Absorbance decreases as the concentration increases

Raw spectra

Mean spectra

Data processing (PLS)

Spectral data (X)

PLS finds a set of orthogonal components that :

maximize the level of explanation of both X and Y
provide a predictive equation for Y in terms of the Xs
fitting a set of components to X (as in PCA)
similarly fitting a set of components to Y
reconciling the two sets of components so as to maximize
explanation of X and Y
X = TP + E
Y = UQ + F

Juice concentration (Y)

This is done by:

Where, T= scores/latent vector of X, P=loadings for X

U= Scores of Y, Q=loadings of Y, E & F =error of X &Y
Data matrix for PLS analysis

Results: Predicted concentration

30

30

25

25

f(x) = 1x + 0
R = 1

20

Calibration predicted value

20

15

Validation predicted values

15

10

10

10

15

20

f(x) = 0.99x + 0.01

R = 0.99

25

30

10

15

20

Actual values

Actual value

Calibration

Predication

25

30

Results

Statists

Calibration

Prediction

0.99

0.98

Error

0.92%

2.60%

PC no.

Predicted bias

-0.092

color

color

Optimal PC no. computed by cross-validation (leave one out)

Beta coefficient of PLS model

Conclusion & further modification

In this project laboratory based spectrometer was developed.
SPLICCO and Csharp was used for data acquisition.
Spectrometer wavelength were calibrated with LED light source.
Orange juice samples with different concentration was measured with the spectrometer.
The absorption data form the spectrometer showed that, as the juice concentration increased the
absorption decreased around 600 nm (associated with the yellow color of the juice).
Furthermore, PLS model was developed for the predication of juice concentration.
The predicted form PLS model was 0.99 and 0.98 respectively, while the SEC and SEP was 0.92 and
2.60%.
Proper focusing and handling was difficult task during spectrometer development therefore in further
modification accurate calibration of spectra and light is highly essential.
In future work LABVIEW program will be developed for calibration and data acquisition.

Thank You !
Chungnam National University, Daejeon, S. Korea
Non-destructive Bio Sensing Laboratory