Professional Documents
Culture Documents
13 Genetic Engineering
Ch. 13 Outline
13-1:
Selective
Breeding
Increasing Variation
13-2:
Manipulating DNA
The Tools
of Molecular Biology
Using the DNA Sequence
Ch. 13 Outline
13-3:
Cell Transformation
Transforming
Bacteria
Transforming Plant Cells
Transforming Animal Cells
13-4:
Transgenic
Cloning
Organisms
What Happened?
During
Genetic
3.
Cloning
Screening
The
Ends
Both
Bind Together
Diagram
Recognition sequences
DNA sequence
Recognition sequences
DNA sequence
Restriction enzyme
EcoRIcuts the DNA
into fragments.
Sticky end
wells.
They
The
Gel Electrophoresis
Power
source
Longer
fragments
Shorter
fragments
Mixture of DNA
fragments
Gel
4.
do this?
Bacterial
pharmaceutical companies
around the world produce important
proteins using genetic engineering.
Vaccine: a solution containing all or part
of a harmless version of a pathogen; used
to prevent viral diseases (dont respond to
drugs)
Many vaccines are made using genetic
engineering
DNA fingerprinting
DNA fingerprinting:
Improving Crops
Genetic
Plant Transformation
Agrobacterium
tumefaciens
Gene to be
transferred
Cellular
DNA
Recombinant
plasmid
Complete plant is
generated from
transformed cell
Animal Farming
Growth
Creating HGH
Recombinant
DNA
Sticky
ends
Bacterial Cell
DNA
recombination
DNA
insertion
Bacterial
chromosome
Plasmid
Animal Farming
Transgenic
Cloning
A donor cell is taken from
a sheeps udder.
Donor
Nucleus
These two cells are fused
using an electric shock.
Fused Cell
Egg Cell
The nucleus of the
egg cell is removed.
An egg cell is taken
from an adult
female sheep.
Embryo
Cloned Lamb
The embryo
develops normally
into a lambDolly
Foster
Mother