Rodenticidal Property of Tubli (Derris elliptica Benth)

Leaves Extract

Researchers: Alejandro Jecino

Cromwell C. Magno
Vanessa D. Viojan

CHAPTER I

THE RESEARCH PROBLEM

Background of the Study

Now a days people are engaged to many
house and agricultural problems because of
rats infestation in our society due to improper
waste disposal.
Rats are the common name for any large
member of a family of rodents, with dullcolored, coarse fur; long tails; large ears; and a
pointed snout.

Background of the Study

Moreover, rats carry a variety of
diseases such as rat bite fever and
bubonic plague, fleas, and salmonella
bacteria.
One way to control rat infestation is by
using rodenticide, Rodenticides are a
broad class ofpesticidedesigned to kill
small mammals such as rats and mice.

Background of the Study

One way to help minimize the use of synthetic
products, the one method is the use alternative
rodenticides by using plants. One of this the
tubli plant (Derris elliptica Benth).
Tubli plant is a rambling climber, with branches
covered with brown hairs. Leaves are pinnate
and 30 to 50 cm. long. Leaflets are usually
oblong, 9 to 13 cm, when matured smooth
above and subgloucous and silky beneath.

Background of the Study

This study was realized when the researchers

found out that the tubli (D. elliptica) has a high

toxicity level. They also learned that tubli plant
has been used as a fish tranquilizer in various
communities.
Through this study, the researchers would like

to determine the rodenticidal property of tubli

(Derris elliptica) leaves extract against rats
(Rattus norvegicus).

Statement of the Problem

The main objective of this study is to prove the
rodenticidal property of Tubli (Derris elliptica) leaves on
rats.
Sub-problems:
1. How effective the Tubli (D. elliptica) leaves crude extract
in killing rats (R. norvegicus) at varying levels of
concentration?
2. Is there any significant difference between the
rodenticidal activities of Tubli (D. elliptica) leaves crude
extract against rats (R. norvegicus) to the commercially
available pesticide?

Null Hypotheses
1. There is no significant difference in the
effectiveness of Tubli (D. elliptica) leaves crude
extract as pesticide in killing rats (R. norvegicus) at
varying levels of concentration.
2. There is no significant difference of Tubli (D.
elliptica) leaves crude extract against rats (R.
norvegicus) to the commercially available
pesticide.

Scope and Limitation

The plant sample and rats will be taken from
Brgy. 93 Bagacay, Tacloban City. The researchers
will use the leaves of the plants for crude
extraction and different concentrations will be
prepared (100%, 75%, 50%).
The crude extract of tubli leaves will be mixed to
rice grain and will be allowed to dry afterwards
the dried rice grain with tubli extract. Finally the
effectiveness of tubli leaves extract as an organic
rodenticide will be tested by using it as bait.

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Definition of Terms
The terms which are used in this study have
been defined for better understanding.
Extract - a concentrated substance obtain
Rats the subject were the Tubli will be tested
Rodenticide a substance that will be used to kill
rats
Tubli the plant that will be tested for its
rodenticidal activity against rats

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CHAPTER II

REVIEW OF RELATED
LITERATURE AND STUDIES

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Review of Related
Derris elliptica Benth was observed to have high
Literature
correlation of esterases and monoxygenases

enzymatic activity and mortality rate when the

plant extract is applied to a diamond back larvae
(Plutella xylostella). The tubli extracts
significantly lowered the number of larvae in the
field.

(Visetson, S. & Milne, M., 2001)

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The sap, combined with Antiaris toxocaria,
used in Borneoas an ingredient of the Kayan
dart and arrow poison or blow-dart poison for
hunting.
(http://philippinetambayan.com/2010/05/12/tu
bli/)

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Analyses showed that the natural rotenone
crystallized for the first time from Derris
trifoliata in Vietnam offered its purity met the
international standard. These evidences indicate
that rotenone can be used and it possibly
replaces chemical pesticides in crop protection
and aquaculture (shrimp culture) to obtain
cleaner vegetable, and shrimp to meet the
demand of sustainable agriculture in the future
in Vietnam. (Phan Phuoc Hien et al, 2003)

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CHAPTER II

METHODOLOGY

Procedure
Independent variable

tu (D. Tubli
elliptica)

Commercial
rodenticide
(dorarat)

Dependent variable
Food only
(grain)

Crude extraction
Concentrations:
100%, 75%, 25%
Preparation of bait

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Rat (Rattus norvegicus)

Rodenticidal property of tubli (Derris elliptica)

leaves extract against rats (Rattus norvegicus).

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Statistical Tool
The researchers utilized the mean in this study in
determining the effectiveness of the tubli (Derris
elliptica) leaves crude extract at different levels of
concentration. Two-way analysis of variance was
used in this study in determining whether if (a) there
is a significant difference in the experimental group
of this research study which is the crude extract of
tubli (Derris elliptica) leaves and if (b) there is a
significant difference between the experimental
group and the control group of this study.

Rodenticidal Property of Tubli (Derris elliptica Benth)

Leaves Extract

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Researchers: Alejandro Jecino

Cromwell C. Magno
Vanessa D. Viojan