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Structure
Life Cycle:
Attachment: Receptor is Nacetylneuraminic acid
Penetration through endosomes
Replication:
mRNA synthesis happens inside the
host nucleus.
Viral mRNA synthesis requires the
activity of at least two influenza virus
polymerase subunits, PB1 and PB2.
Viral replication-transcription complex
splice the host mRNA cap and use it
as primer for viral mRNA. (Cap
snatching or stealing)
Using host splisosome the viral mRNA
is spliced to 10 segments. Each
segment now translate for a protein.
Pox virus
DNA (ds) virus
Replicate in the cytoplasm and are independent
of the host for mRNA production
Large animal virus infect vertebrate and
invertebrate
Mammal, Birds and insects
RNA polymerase.
Early transcription factors.
mRNA capping enzymes.
mRNA poly(A) polymerase.
RNA helicase.
DNA helicase, ligase, and topoisomerase.
Protein kinase(s)
The pox virus genome consists of linear double-stranded DNA. The vaccinia virus
genome has about 185 kbp and about 180 genes.
Pox virus DNA is unique because the two strands of the double helix are cross-linked
at their termini as a result of phosphodiester bonds between adjacent strands
Replication
It is the only DNA viruses that replicate in a defined area
within the host-cell cytoplasm, a so-called virus factory
Entry : receptors for the poxvirus are thought to be
Glycosaminoglycans (GAGs).
Primary un coating
1.
2.
3.
4.
5.
6.
On release into the host-cell cytoplasm, the core synthesizes viral early
mRNAs which are translated by the cellular protein-synthesizing machinery
Secondary un coating
Coat disassembly
Early proteins produced, catalys the viral genome replication and expression
of intermediate genes
Intermediate gene products controls late genes
Late genes produce viral capsid proteins and other enzymes
The initial assembly reactions result in formation of the immature virion which
is a spherical particle delimited by a membrane that may be acquired from an
early compartment of the cellular secretory pathway.
This virus particle matures into the brickshaped IMV
The IMV can be released by cell lysis
Or IMVs acquire a second, double membrane from a trans-Golgi or early
endosomal compartment to form the intracellular enveloped virion (IEV)
IEVs move to the cell surface on microtubules where fusion with the plasma
membrane forms cell-associated virions (CEV). These CEV induce an actin
polymerization that promotes a direct transfer to surrounding cells or they
can also dissociate from the membrane as EEV.
Glycosaminoglycans
(GAGs)
Small pox: