Professional Documents
Culture Documents
Tujuan pembelajaran
Menjelaskan respon imun terhadap
plak
Menjelaskan plak (sifat fisis,
mikroorganisme)
Komposisi plak
Mikroorganisme 1
gr plak tdd > 500 spc
Mycoplasma, yeasts,
protozoa, virus
Matriks interseluler
(20-30% massa plak)
Organik dan anorganik
dari saliva, GCF,
produk mo
Organik
glikoprot saliva,
polisakarida mo,
albumin GCF, food
debris
Anorganik Ca, P,
Na, K, F
Pembentukan pelikel
Formation of the dental pellicle on the tooth surface
is the initial phase of plaque development surfaces
of the oral cavity, including all tissue surfaces as
well as surfaces of teeth and fixed and removable
restorations, are coated with a glycoprotein pellicle.
This pellicle is derived from components of saliva
and crevicular fluid as well as bacterial and host
tissue cell products and debris.
Kolonisasi
Secondary colonizers are the microorganisms that do not
initially colonize clean tooth surfaces, including Prevotella
intermedia, Prevotella loescheii, Capnocytophaga
spp., Fusobacterium nucleatum, and Porphyromonas
gingivalis.
These microorganisms adhere to cells of bacteria already
in the plaque mass.
Extensive laboratory studieshave documented the ability
of different species andgenera of plaque microorganisms
to adhere to one another,a process known as
coaggregation.
This process occursprimarily through the highly specific
stereochemicalinteraction of protein and carbohydrate
molecules locatedon the bacterial cell surfaces" ," in
Biofilm is a complex
substance.
A biofilm is a complex
aggregation of
microorganisms
growing on a solid
substrate. Biofilms are
characterized by
structural
heterogeneity,
genetic diversity,
complex community
interactions, and an
extracellular matrix of
polymeric substances.
Formation of Biofilms
Biofilms may form
on living or nonliving surfaces,
and represent a
prevalent mode of
microbial life in
natural, industrial
and hospital
settings
Mechanisims of Biofilm
formation
Formation of a biofilm
begins with the
attachment of freefloating microorganisms
to a surface. These first
colonists adhere to the
surface initially through
weak, reversible van der
Waals forces. If the
Steps in Biofilm
Development
Biofilm development can
be divided into several
key steps including
attachment, micro
colony formation, biofilm
maturation and
dispersion; and in each
step bacteria may recruit
different components
and molecules including
flagella, type IV pili, DNA
and exo polysaccharides.
Stages of biofilm
development.
Steps in Biofilm
formation
Dental plaques
The formation of
dental plaque bio
films includes a
series of steps that
begins with the
initial colonization
of the pellicle and
ends with the
complex formation
of a mature bio film.
Biofilms everywhere
They're everywhere:
on your shower
curtain, on medical
devices implanted in
patients, on rocks in
rivers and streams,
and in your nose.
While the sheer
number of different
organisms a biofilm
may contain makes it
a challenge to study,
CDC on Biofilms
Biofilms form on the surface of
catheter lines and contact lenses.
They grow on pacemakers, heart
valve replacements, artificial joints
and other surgical implants. The CDC
(Centers for Disease Control)
estimate that over 65% of
Nosocomial (hospital-acquired)
infections are caused by biofilms.