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Growth
Dra. Sitti Zuleiha
Growth
of bacteria
Nutrients
- Water :
Bacteria need water in high
concentration to grow up and
for circulation in cells.
.
- Anorganic material :
Stabilized coloid and osmotic
pressure.
Stabilized balance alkali acid
activator enzym reaction
- Mineral :
Sulfur
Phosphorus
Source :
Phosphate (PO4) is required as a
component of ATP, nucleic acids, and
such coenzymes as NAD, NADP and
Flavins.
Activator
Nitrogen Source :
Sources of Nitrogen in microbial
nutritions : NO3, NO2, N2, NH4, RNH2 (organic radical).
Carbon Source :
Some bacteria are able to
photosynthetic energy to reduce
CO2.
2. Heterotrophs
- Photosyntetic heterotrophs bacteria
require light energy.
- Chemosyntetic heterotrophs
bacteria : require organic carbon for
growth, and the organic carbon must be
in a form that can be assimilated.
Reproduction of bacteria
Occurred : - Asexual
Sexual
Asexual : 1. Cell division
2. Formation of bud /
branch
3. Formation of filament
1. Cell division
Commonly, growth of bacteria
with amytosis to be binary division.
Generation Time is the time
between two division 20 minutes
15 hours.
GT: M.tuberculosa 15 hours.
3. Formation Filament
The cell was out long filament,
this filament dont have branch. After
that the chromosom was in to
filament filament cut off some
divisions. One division formed the
new bacteria. The situation find in
abnormality.
Exp : Haemophylus influenza
Sexual :
The union of two chromosom
bacteria
new cell bacteria, genetic same
mothers chromosome
That situation find the same
type
bacteria in one family.
Exp : Enterobacteriaceae :
E. coli with Shigella dysenteriae
E. coli with Salmonella thyposa
2. Logarhytmik phase
This phase has been during 18-24
hours.
Growth of bacteria very ideal and
constant
3. Stationary phase.
There is a slow loss of cells through
death, which is just balance by the
formation of new cells through
growth and division.
4. Decline phase
The majority of cells have died,
the death rate decreases drastically,
so that a small number of survivors
may persist for months or even year.
Negative growth.
Variability :
Cohn & Koch : Success to get the pure
culture of bacteria and said the bacteria
always have the same form ( teory
monomorfisme).
Bacterial can be changed form and
physiology depend on the environment.
Involution :
A change with bacterial retardation,
usually find the old bacteria.
Adaptation
The patogenicity of the bacteria could be
adapted, according to the difference
environment.
Decreasing pathogenicity will be recovery
when they are cultured to the animal
model.