BSE222A/612A/341

Biochemical Engineering

ASHOK KUMAR
Department of Biological Sciences and
Bioengineering
Indian Institute of Technology Kanpur
Semester-II (2015-2016)

BSE222A/612A/341
Biochemical Engineering
Course Contents
Biochemical engineering: Upstream and Downstream
Bioseparations and bio-processes, Downstream processing.
Characteristics of biological materials, pretreatment methods;
separation of cell biomass, adsorption, filtration, centrifugation,affinity
bases separation, polishing-crystallization, drying, case studies;
Process integration. Bioprocess integration for efficient production and
recovery, scale-up consideration, process monitoring and process
economics
Biochemical engineering: Stoichiometry and energetics of microbial
metabolism. Transport phenomena. Enzyme catalyzed reactions and
processes. Bioreactor design and applications. Sterilization.
Instrumentation and control
Environmental bioprocesses. Interaction of mixed microbial population,
applications, biological wastewater treatment, anaerobic, digesters,
bioremediation.

List of Books
Biochemical Engineering Course
1. Blanch, H. W. and Clark, D. S. Biochemical Engineering.
Marcel Dekker, Inc.,
2. Bailey, J. E. and Ollis, D. F. Biochemical Engieering Fundamentals.
McGraw-Hill, Inc.,
3. Belter, P. A., Cussler, E. L. and Hu, W. S. Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons
4. Desai, Mohamed. A. Downstream Processing of Proteins:
Methods and Protocols. Humana Press
5. Shuler, M. L. and Kargi, F. Bioprocess Engineering- Basic Concepts

Evaluation
1. Attendance

10%

2. Mid sem Exam

35%

3. Quiz , assignment & presentations

15%

4. End sem Exam

40%

Bioprocess engineering/Biochemical engineering

The efficient utilization of physical, chemical and biological
processes to convert raw materials into useful products, at
minimal cost, with minimal energy consumption, and with
minimal impact on the environment.

Bioprocess/Biochemical
Engineering
Process for developing useful products by taking
advantage of natural biological activities.
Classical example include making alcoholic beverages
the yeast cells and nutrients (cereal grains) formed a
fermentation system in which the organisms
consumed the nutrients for the growth and produced
by-products (alcohol).
Today's modern bioprocess technology is based on
the same principle: combining living matter (whole
organisms or enzymes) with nutrients under the
conditions necessary to make the desired end
product.

Bioprocessing
Many potential uses of biotechnology are
developed through laboratory procedures
that generally produce only small amounts of
useful substances.
As advances in bioprocess technology,
particularly, fermentation, separation and
purification techniques, are madecommercial firms are able to economically
produce these substances in large amounts

Bioprocessing
Because bioprocesses use living
material, they offer several
advantages over conventional
chemical methods of production:
They usually require lower temperature,
pressure, and pH.
They can use renewable resources as raw
materials; and greater quantities can be
produced with less energy consumption.

Bioprocessing
In bioprocesses, enzymes are used to catalyze the
biochemical reactions by whole microorganisms or
their cellular components.
The biological catalyst causes the reactions to
occur, but is not itself changed.
After a series of such reactions (which take place in
large vessels called fermentors or fermentation
tanks), the initial raw materials are chemically
changed to form the desired end product.