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m p
plants can adjust their metabolism
to cold and increase their tolerance to freezing in
response to low-non-freezing temperatures (Levitt,
1980).
p
²
²
²
pthrough:
÷olerance of freeze-induced cellular dehydration via
accumulation of various solutes in cell sap
S
÷emp. range -16 to -25 0c
÷ightly packed cells and reduced intraacellular spaces allow
supercooling in cold acclimated plants
²avoured by loss of water during cold acclimation by small cells.
Specifically parenchymal cells survive by tolerance to freeze-
induced dehydration process.
Solutes such as soluble sugars (sucrose, glucose, raffinose, fructose,
etc.) in the vacuoles improve the water holding capacity of cells and
enables avoidance of stress caused by freeze-induced dehydration.
m ÷hey have the ability to adsorb onto the surface of ice crystals
and inhibit their growth and recrystallization.
m In addition p# p#
, two genes whose
expression is transiently induced by cold and in response
to drought, salt stress and ¯~¯, were also identified (Capel
et al., 1997; Medina et al., 2001).