Professional Documents
Culture Documents
FOOD INDUSTRY
Flavor enhancers, MSG, glycine, alanine. Tryptophan and histidine
act as antioxidants to preserve milk powder. For fruit juices
cysteine is used as an antioxidant.
Aspartame,
dipeptide
(aspartyl-phenylalanine-methyl
ester)
produced by combination of asp and Phe is 200 sweeter than
sucrose. Used as low calorie artificial sweetener in soft drink
industry
Essential amino acidsor those deficient in plant based foods like
lys, met, thr, Trp improves nutritional quality of food and feed
additives (animal). Bread: lysine, soy products or soyabean meal
(pigs/animals): methionine
PHARMACEUTICAL INDUSTRY
L-GLUTAMIC ACID
arabinogalactan
L-GLUTAMIC ACID
Metabolic pathway
the
Glu
biotin
biotin
Phosphoenol pyruvate
carboxylase
PDH
Isocitrate
dehydrogenase
L-GLUTAMIC ACID
Regulatory control:
Good supply of glucose and efficient conversion of phosphoenol
pyruvate to oxaloacetate
Phosphoenol pyruvate carboxylase and pyruvate carboxylase, pyruvate
dehydrogenase
a-ketoglutarate dehydrogenase (low activity by adding penicillin,
surfactants)
Glutamate dehydrogenase (high activity)
1 mole of glucose should produce 1 mole of glu
In practice, efficiency is 70%
Carbon sources
Nitrogen source: ammonia for carbon to glu pH control
Growth factors :biotin
O2 supply: high conc inhibits growth and low O2 leads to lactic
acid production and succinic acid, Afftects Glu production in both
cases
Nutrients
Glucose (12%)
Dissolving tank
38oC
30-35h
Sterlizer
Buffer tank
Inoculum
Sterile air
FERMENTER
Cell separator
Eluted in NaOH
Anion exchanger
Evaporation
Crystallization
100g/L