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metabolism
Learning outcomes
Upon completion of this chapter,
students should;
Understand
Understand
Understand
Understand
glycogenolysis
glyconeogenesis
Cori cycle
pentose phosphate pathway
Short review
What will happen if you have excess
glucose?
Glucose is converted to glycogen in the
liver
Glycogen is then stored in liver and
muscle
Short review
What happens when you have less glucose?
glycogen
phosphorylase
HO
HO
HO(Glucose) n-1OH + H2 O +
CH2 OH
O
OH
OPO32-D-Glucose-1-phosphate
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Glycogenolysis
The breakdown of stored glycogen to
glucose when the blood sugar is low.
Is glycogen actually broken down to
glucose?
Not really!!! It is broken down to glucose
-1-phosphate
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Glycogenolysis
First enzyme that we should look at is the
glycogen phosphorylase
Breaks down the -(1-4)- glycosidic linkages
between the glucose molecule in glycogen
LIMITATION: cannot breaks down 4 or less
glucose residues.
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Glycogenolysis
The second enzyme will come to the rescue glycogen
debranching enzyme
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Glycogenolysis
The end products from the break down
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Glycogenolysis
In muscle glucose-6-phosphate is used to produce
energy, whereas in the liver it is ultimately transported to
other tissues via the circulatory system
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Gluconeogenesis
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Gluconeogenesis
Gluconeogenesis: the synthesis of glucose from pyruvate
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Gluconeogenesis
Step 1: carboxylation of pyruvate
requires biotin
pyruvate carboxylase is subject to
allosteric control; it is activated by
acetyl-CoA
O
biotin
pyruvate
carboxylase
O
CH2 CCOO- + ADP + Pi + 2H+
COOOxaloacetate
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Biotin
Biotin is a carrier of carbon dioxide
O
HN
NH
1. H2 N-enzyme
2. CO2 + ATP
COO-
S
Biotin
O
-
NH
H
S
C
O
NH-enzyme
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Gluconeogenesis
decarboxylation of oxaloacetate is
coupled with phosphorylation by GTP to
give PEP
O
CH2 CCOO- + GTP
CO2Oxaloacetate
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Gluconeogenesis
Second different reaction in
gluconeogenesis
Gluconeogenesis
Third different reaction in gluconeogenesis
HO
HO
CH2 OPO32O
+ H2 O
OH
OH
-D-Glucose-6-phosphate
glucose-6phosphatase
HO
HO
CH2 OH
O
OH
OH
-D-Glucose
+ Pi
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Glycogen metabolism
control
Glycogen phosphorylase - a
major control point
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Glycogen metabolism
control
Glycogen metabolism
control
Glycogen synthase also exist in two forms
Phosphorylated
synthase D
(inactive)
glycogen
Non-phosphorylated
synthase I
(active)
glycogen
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Intro to PPP
The main purpose for this pathway is to
produce
NADPH provides reducing power for
biosynthesis
ribulose-5-phosphate to make nucleic acid
Oxidative PPP
Involves two steps:
1. G6P ribulose-5-P + CO2 (irreversible)
2. 2NADP+ 2NADPH
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CHO
+
H
OH NADP
HO
H
H
OH
H
OH
CH2 OPO32 Glucose-6-phosphate
COO+
NADPH
H
OH NADP NADPH
HO
H
H
OH
H
OH
CH2 OPO326-Phosphogluconate
COOH
OH
C O
H
OH
H
OH
CH2 OPO32 -
CH2 OH
C O
+ CO2
H
OH
H
OH
CH2 OPO32 Ribulose-5-phosphate
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Non-oxidative pathway
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End of Lecture
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