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Methylotrophs (PPFMs)
N. Karthikeyan
Scientist
ICAR-NBAIM
MAU
Methylotrophs
Compounds used for methylotrophic
growth include:
Methane
Methanol
Formaldehyde
Formate
Methylamine
Trimethylamine (multicarbon but without
C-C)
Methylotrophs
Obligate methylotrophs cannot grow on multicarbon
compounds (include most methanotrophs)
Facultative methylotrophs also grow on multicarbon
compounds (eg. Methylobacterium extorquens = Pseudomonas AM1
Restricted facultative methylotrophs grow on C1 or C2
compounds (eg. Hyphomicrobium)
There is a huge number of different types of methylotroph. Very
few are typical bacteria that had been described previously.
Although their oxidative metabolism is basically similar, there
are many different carbon assimilation pathways.
Most are Gram-negative. The few Gram positive methylotrophs
(Bacillus sp) have different oxidation enzymes
Methylotrophs
1906
1961
facultative methyltroph
1964
1970
1971
1982
C1 carbon assimilation
Methylotrophs assimilate C1 carbon
source via either ribulosemonophosphate pathway or the
serine pathway
Serine Pathway
Plant Probiotics
Probiotics are: Live
microorganisms which
when administered in
adequate amounts
confer a health benefit
on the host.
It should come as no
surprise that we
humans arent the only
organisms that benefit
from relationships with
the right kind of
bacteria.
and growth.
application
Methylobacterium
Members of the Methylobacterium genus are
classified as
-proteobacteria and include 51 reported species
(http://
www.bacterio.net/methylobacterium.html)
This genus is composed of Gram-negative bacteria
that are generally with pink pigmentation due to
carotenoid synthesis rod shaped, strictly aerobic
and able to grow using compounds containing only
one carbon (C1), such as methanol and
methylamine.
Thus, these bacteria are denoted pink-pigmented
facultative methylotrophs (PPFMs).
Methylobacterium
The main characteristic of this group is the ability to
oxidize methanol using the methanol dehydrogenase
enzyme (MDH)
The mxaF gene encodes the large subunit of this
enzyme, which is key in methylotrophic metabolism and
is used to study that group of bacteria
Members of the Methylobacterium genus occupy
different habitats due to their great phenotypic plasticity,
including soil, water, leaf surfaces, nodules, grains, and
air
They can also be opportunistic pathogens in human
Methylobacterium
Growing in meristematic tissue, they can reach
populations of 104 to 106 colony-forming units (CFU) per
gram of plant tissue
In addition, they can form biofilms and use
methylotrophic metabolism as an adaptive advantage
during plant host colonization
Methylobacterium
The association between Methylobacterium spp. and host
plants varies from
and to intermediate or
endophytic
Dourado et al
Dourado et al
2015
Phyllospher
e
The aerial parts of living plants including leaves, stems,
buds, flowers and
called as Phyllosphere
The term phyllosphere coined by Ruinen (1961)
Phyllospher
e
Caulosphere
(stems)
Phylloplane
(Leaves)
Anthospher
e (Flowers)
Caprosphere
(Fruits)
Phyllospher
e
Cytokinin
production
Success
story
201
2
Success
story
Seed treatment Imbibe seed in 1.0 % volume for 5-10min (depending on seed)
Foliar Spray of 1% PPFM
Spray during morning or evening
Recommended for all crops
Spray at critical stage of crop growth (or) 30days interval
Precaution : Do not mix with pesticide / fungicide
Thank you