Professional Documents
Culture Documents
of Mass Spectrometry
BS221 2008
University of Essex
and MALDI
Ion Source
Mass Analyzer
Detector
Data Station
Types of ionization
Electron Impact - sample is heated and energized by
a beam of electrons, usually gives a molecular ion
(M+) and a lot of fragments
Types of ionization
Matrix Assisted Laser Desorption Ionization
(MALDI) sample is co-crystallized with a matrix and
then irradiated with laser. Singly charged (M+H)+ and
(M-H)- ions are produced. Good for intact proteins.
Types of ionization
ElectroSpray Ionization sample is sprayed out of a
narrow nozzle in a high potential field. Generates
positive (M+nH)n+ and negative (M - nH)n- ions and
almost no fragmentation. Generates multiple charged
ions.
(1)
v = (2E/m)1/2
(2)
Quadrupole analyzers
Q-TOF
Triple Quad
Full scan
Precursor isolation
Fragmentation
Detection
Auto MS/MS
The instrument alternates
between MS and MS/MS
mode selecting precursors
according preset criteria
MS analysis of biomolecules
Small molecules: drugs, drug breakdown products, sugars, lipids, amino
acids, fatty acids and their esters
EI and CI (chemical ionization), ESI, are used predominantly in combination
with sector, quadrupole and time-of-flight mass analyzer