Professional Documents
Culture Documents
WARNING!!!! THIS
SHOULD NOT REPLACE
YOUR NOTE OR TEXTBOOK
IN ANYFORM.
Medical microbiology
It is concerned with
Aetiology
Epidemiology
Pathogenesis,
Diagnosis
Prevention
Control of microbial infection
Types of infections
Abscess
Acute infection
Bacteremia
Septicemia
Chronic infection
Overt infection
Focal infection
Latent infection
Localized infection
Mixed infection
Nosocomial infection
Pyogenic infection
Systemic infection
Toxemia
zoonosis
Pathogenesis of bacterial
disease
Microbial products
Coagulase S.aureus
Colagenase Cl.spp
DNAsestaph,groupA
streptococcus,Cl.pefringes
Haemolysinsstaph, E.coli, enterococcus, strept
Hyaluronidase staph, Cl
Hydrogen peroxide staph
Streptokinase group A streptococcus
Leucocidin staph, pneumococci, streptococci
Respiratory tract
Staph aureus,
Branhamella catarrhalis
Haemophilus influenza
Mouth
Actinomycese, Lactobacilli,
Neiserria, Staphylococcus,
Heamophillus and streptococcus
Stomach
Helicobacter pylori
Intestine
Genitourinary tract
Staphylococcus
Commensals of the skin, nose, mucus membrane and
respiratory tract
Contracted from asymptomatc patient
Boils, Carbuncles, Folliculitis, scaled skin
Produce disease due to extracellular enzymes such as
B lactamase penicillin
Catalase- phaogocytosis
Haemolysin, liposis, nucleases, proteases
Staph au
Coagul
positive
Produc
yellow
on app
media
Manito
streptococcus
S.pneumoniae, S.pyrogenes and
S.galactiae
Most infections of man are caused by
members of groupA Streptococcus
(S.pyrogen)
Streptococus pyogens
Sore throat(pharyngitis)
Fever, Sore-throat, Swollen cervical lymph nodes and exudates from the
Throats (FSST)
Scarlet fever
Streptococcal pyrogenic toxin (SPE)Erythrogenic toxins- Blood-Spleen-Reddish
rash on skin(EBSR)
Streptococcal toxic shock like syndrome
Higher fever, erythema and often a skin rash that may become gangrenous- necroticRenal failure and pneumonia are complications(FESGNR)
Skin infections
May lead to lesions if there is prior injury of insect bite
Diagnosis
Throat swab, skin swab, B.agar alpha and beta haemolysis
Diff staph from strep by oxidase and catalsase (both neg for strept.)
Streptococcus pneumoniae
Enteric infection
This includes
Salmonella
Shigella
Yersinia
E.coli
Shigellosis (Bacilliary
infection)
Symptoms
Abdominal pains/cramps, diarrhea, fever, watery stool
(blood and mucus)
Dehydration and electrolyte imbalance may lead to death
Diagnosis
Isolation of the organism from faeces
Travellers diarrhea
It is a rapidly acting dehydrating condition, nuisance to
international travellers, it is xterised by profuse watery
diarrhea
ETEC enterotoxigenic e.coli
Produces heat stale and heat labile enterotoxix
EHEC enterohaemorrhagic
Causes hemorrhagic colitis, produces shiga toxin
salmonella
S.typhii, S.enteritidis, S.typhimerium,
S.paratyphii, S.choleraensis
S.typhimerium causes
salmonellosis/gastroenteritis
Reservoir (I.T of birds and animals)
Egestion of contaminated food
Incubation period of 8-48hrs
Invades and multiplies in submucosa
Produces enterotoxin that destroys the epithelial
cells
Symptoms
Abdominal pain, diarrhea, nausea and vomitting
S.typhii
causes typhoid fever/enteric infection
Consumption of contaminated food and water
Penetrates the epithelial lining of the small
intestine
Survives phagocytosis
1st week is xterised by fever, cramps and malaise
2nd week it infects the gall bladded
It sheds back into the intestine
Symptoms
Ulcerative lesion, abdominal tenderness, diarrhea,
vomitting exhaustion and death
Diagnosis
Widal test
Faeces blood and urine are the specimen used
Cholera
01
0139
Classic
El Tor
Diagnosis
Culturing stool sample using blood agar and thiosulphate citrate bile
salt, the organism grows with a yellow color