Professional Documents
Culture Documents
MYCOLOGY
Classification of fungi
Morphological
Clinical
classification
Systematic
classification
General mycology5
Yeast
s
Oval or round cells that reproduce by
budding to form blastospores or
blastoconidia.
May form pseudohyphae.
Examples: Candida, Cryptococcus.
General mycology7
Yeast cells
General mycology8
Molds
Also called filamentous fungi or
mycelial fungi.
The filaments called hyphae.
Hyphae interlace to form mycelium.
General mycology9
Hyphae
General mycology11
Types of
hyphae
General mycology12
Dimorphi
c fungi
These fungi occur in two forms:
1.At the room temperature (22 degree),
filamentous form (Saprobic phase).
2.In the body (37 degree), yeast form
(Parasitic phase).
Examples: Histoplasma, Blastomyces,
Coccidiodes.
General mycology13
Clinical
classification
14
Superficia
l mycoses
Fungal infections confined to the stratum
corneum without tissue invasion.
Cutaneous
mycoses
Fungal infections that involve
keratinized tissues as skin, hair, nail.
Examples are: dermatophytes &
cutaneous candidiasis.
Subcutaneous
mycoses
Fungal infections that are confined to
subcutaneous tissues without
dissemination to distant sites.
Examples are: mycetoma,
chromomycosis, sporotrichosis.
Systemic
mycoses
Also called endemic mycoses.
Begin as primary pulmonary lesions
that may disseminate to any organ.
Caused by dimorphic fungi.
General mycology18
Opportunistic
mycoses
Affect immunocompromised individuals
Examples are:
1.
2.
3.
4.
5.
Systemic candidiasis.
Cryptococcosis.
Aspergillosis.
Mucor & rhizopus.
Pneumocystis infections.
General mycology20
Allergy
Allergy occurs to fungal spores
particularly those of aspergillus and
alternaria fungi.
Allergy is mostly type hypersensitivity
such as bronchial asthma.
Toxicosis
1-Mycetismus
toxic. Amanita
2Mycotoxicosi
s
Ergotism which is caused by the mold
Claviceps purpura. This mold infects
grains and produce alkaloids (ergotamine
and LSD) that cause neurological effects.
Aflatoxins produced by Aspergillus flavus
which infects grains and peanuts. This
toxin is hepatotoxic and suspected of
causing hepatic carcinoma.
Systematic
classification
It is based on the type of
sporulation, sexual & asexual.
Systematic classification
Blastoconi
dia
Microconidi
a
Macroconidi
a
Arthrospor
es
Chlamydospor
es
Sporangiospores
Laboratory diagnosis
1-Specimen:
Skin scales, nails, hair clippings for
dermatophyte examination.
2-Microscopic examination of these
specimens using KOH 10%:
KOH dissolves keratin but does not
affect fungi. Branching hyphae are
detected among epithelial cells.
Types of fungal stains:
*Lactophenol cotton blue
* Giemsa stains trophozoites of
Pneumocystis jiroveci.
* Toluidine blue stains cysts of
Pneumocystis jiroveci.
5-Culture:
Morphology of the
colony:
color on surface and
reverse
1-Direct examination
2-Slide culture
Germ tube
Chlamydospore
Serological
tests