Professional Documents
Culture Documents
Evy Sulistyoningrum
Histology Department
Medical Faculty of UNSOED
Outlines
Introduction
Hypophysis
Adrenal glands
Thyroid
Parathyroid
Pancreas
Pineal gland
Regulatory system
Nervous system
Neurotransmitters
Direct
communication
via synapsis
Brief response
Transient effect
Endocrine system
Hormones
Indirect
communication via
bloodstream
Longer response
Long lasting effect
Endocrine Glands
Structure
Ductless x Exocrine
Ectodermal or endodermal origin
Hormones secreted directly into the
vascular system (circulation)
Glands often have special
microcirculation
Secretions absorbed from interstitium
into blood
Target cell express proper receptor
Endocrine vs Exocrine
Gland
ENDOCRINE GLANDS
HYPOTHALAMUS
GLAND
PARATHYROID
Other non-specific
endocrine glands
Stomach
Heart
Kidney
Thymus
Gonads
Hypothalamus
Control Center for internal
environment
Acts via 3 mechanisms:
1. ANS centers exert nervous control on
adrenal medulla
2. ADH and oxytocin production (direct
acting)
3. Regulatory hormone production (RH
and IH) controls pituitary gland directly
and all other endocrine glands
indirectly
Hypothalamus
Consists of neurons arranged into nuclei
Neurons secrets many hormones in
neurosecretoric vesicles
Neurosecretoric vesicles are transported
via axon to:
Hypothalamohypophyseal tract
released absorbed by capilarries
transported into hypophysis (IH or RH)
Pass hypothalamohypophyseal tract
Neurohypophysis released (ADH &
oxytocin)
Hypothalamic
hormones/factors
TRH
CRH
SRH
GnRH
PRH
PIF
ADH
Oxytocin
The Pituitary
Hypophyses
Location: sella tursica
Was known as the major regulation of
other endocrine tissues (The Master
Gland)
Thyroid
Adrenal gland
Gonads
Others
2 Major area: (embryological based)
Adenohypophysis (oral ectoderm)
Neurohypophysis (neural
ectoderm)
Embryology of hypophysis
Hypophysis
Lobus
Pars distalis Anterior
Pars tuberalis
Pars intermedia
Adenohipofisis
Lobus
Poster
Neurohipofisis Pars nervosa ior
Infundibulum
Eminentia mediana
Hypophysi
s
Zona
Intermedia
Hipofisis anterior/Adenohipofisis
Hipofisis
posterior/Neurohipofisis
Hipofisis
anterior/Adenohipofisis
Hipofisis
posterior/Neurohipofisis
Zona Intermedia
Adenohypophysis
Pars distalis
Pars intermedia
Pars tuberalis
Adenohypophysis: pars
distalis
Fibrous capsule
Parenchymal cells : epithelial gland
cells formed as chord
Sinusoidal capillaries +++
Fenestrated endothelial lining
Cells
Cell types (based on dyes affinity)
Chromophils
Acidophils (>>>)
Basophils
Chromophobes
Small cell with very little
cytoplasm dont take up stain
well, not produce any hormone
Undifferentiated stem cell
Degranulated chromophils
Phagocytic cell
Adenohypophysis: pars
distalis
Adenohypophysis: pars
distalis
Adenohypophysis: pars
distalis
Cells (based on secretory granules
within)
Acidophils
Somatotrophs
GH (somatotropin)
Secretory granules: 300 nm in diameter
Centrally placed nucleus
Mitochondria, Golgi, RER >>
Mammotrophs
Prolactin
Secretory granules: 600-900 nm in
Thyrotrophs
TSH
Secretory granules 60-160 nm in diameter
Gonadotrophs
FSH & LH
Cellls of adenohypophisis
Adenohypophysis : Pars
intermedia
Between pars distalis
and pars nervosa
Cuboidal cells
Rathkes cysts (colloid
containing cysts)
Zona intermedia (in
human)
Basophilic cells
Produce POMC
MSH
Corticotropin
-Lipotropin
-Endorphin
Pars Intermedia
Epithelial origin
Contains cells
similar to
corticotrophs
(basophillic cells)
Not well defined in
the human
Often has cystic
structures contain
colloid
Adenohypophysis
Pars tuberalis
Surrounds
hypophyseal stalk
Highly vascularized
Cuboidal or lowcolumnar basophilic
cells
Small dense
granules
Colloid droplet
Glycogen
No spesific hormones
ADENOHYPOPHYS
IS
TROPHIC HORMONES
(TROPHINS)
ON PERIPHERAL TISSUES
Growth Hormone
(GH)/Somatostrophin)
Adrenocorticotropic Hormone (ACTH)
Prolactin (Luteotrophi
Hormone/LTH)
Follicle Stimulating Hormone (FSH)
Luteinizing Hormone (LH)
PARS INTERMEDIA*)
(MSH)
Neurohypophysis
Posterior pituitary
Down-growth of hypothalamus
Hypothalamohypophyseal
tract
Axons of neurosecretory
cells extend into the
posterior hypophysis
Neurosecretory cells:
paraventricular & supraoptical
nuclei
Direct acting hormone: ADH,
Neurohypophysis
Non-myelinated nerve fibers (axons) from
neurosecretory cells in hypothalamus
Distentions of axon containing neurosecretory
granules: Herring bodies
Capillaries
Supportive cells: pituicytes (neuroglial cells)
lipid droplet, lipochrome pigment, intermediate
filaments
Cytoplasmic processes
25% volume
Basophillic invasion
Neurohypophysis
Herrings
body
1 = neuron fibers
2 = pituicytes
3 = herrings body
NEUROHYPOPHYSIS
SUPRAOPTIC
NUCLEUS
(SON)
Blood
circulation
ADH
ADH/
OXY
NEUROHYPOPHYSIS
(sinusoids)
NEURO
SECRETORY
CELLS
(hypothalamus)
PARAVENTRICULAR
NUCLEUS (PVN)
(ADH: antidiuretic
hormone/vasopressin)
OXYTOCIN
VASOPRESSIN (ADH)
SON
(OSMORECEPTOR)
COLLECTING TUBULES
INCREASED OSMOTIC PRESSURE
OF THE BLOOD (Haemoconcentration)
INCREASED REABSORPTION
OF WATER
STRENOUS WORK
1.
CONCENTRATED URINE
(retain water:haemodilution)
RF/IF
ADENOHYPOPHYSIS
(cords/clumps of endocrine cells)
Hypothalamohypophyseal
portal system
Trophic hormone
(Trophin)
TARGET GLAND
TARGET CELLS/TISSUES
HORMONE
LOCATION
ANTERIOR AND LATERAL TO THE TRACHEA AND
LARYNX
BUTTERFLY-SHAPED
2 LATERAL LOBES
ISTHMUS
OUTER CAPSULE (dense connective tissue)
LOBULES
(Contain: numerous follicles,
lined by a simple cuboidal epithelium
THYROID HORMONE (THYROXIN)
BOUND TO TRACHEA BY PRETRACHEAL FASCIA
MOVES WITH LARYNX (when swallowing)
Thyroid glands
Stores large amount of
hormone
Functional unit : the
follicle
simple cuboidal
epithelium
Contain colloid
inactive hormone
bound to a
glycoprotein
(thyroglobulin)
Thyroid
Follicular cells
Endodermal origin
Squamous to columnar cell (Height of
cells proportional to activity)
Round to ovoid nucleus
Short villi projecs to lumen
Basophilic cytoplasm
RER
Apical lysosomes
Mitochondria
Pre-hormone storage as extracellular
fluid in lumen (colloid)
inactive
active
Parafollicular cell
C cell
Between follicular
cells
Clear cytoplasm
clear cell
Contain small
secretoric
granules
Produce calcitonin
blood calcium
homeostasis (with
Parathormone)
THYROID GLAND
THYROID GLAND1
2. para(T3)
follic. cell
1. follicular Cell
THYROXIN (T4)
TRIIODOTHYRONINE
HYPERTHYROIDISM OVERPRODUCTION OF
OF THYROXIN
Metabolic rate increase
2. THYROCALCITONINE
(CALCITONINE)
(nervous, weight loss, tremor,
tachycardia, active,
intolerance to heat, sweating easily)
CONTROLLED BY
HYPOTHYROIDISM INSUFFICIENT AMOUNT
SERUM CALCIUM LEVEL
OF THYROXIN
Metabolic rate decrease
(sluggish, long sleeping-16 hr/day, body weight
increase, falling hair, scaly skin, decreased heat
production and sensitivity to cold, myxedema)
PARATHYROID GLAND
Endodermal origin
2 superior + 2 inferior, at the
posterior aspect of thyroid gland
Separated by connective tissue
with the thytoid gland
5 mm in length, 4 mm wide, 2
mm thickness
Fibrous capsule extended into
septa
Clusters of epithelial cells : chief
cells & oxyphil cells
Supported by reticular fiber
Parathyroid gland
Parathyroid
Chief cells
Oxyphil cells
Larger cell
More lightly eosinophilic cells
Unknown function
Parathyroid
Parathyroid
Parathyroid
Function
Together with calcitonin
regulate calcium and phosphorus
levels
Primarily responds to decreased
calcium in order to raised blood
calcium level
Increase absorption of calcium
from intestine
bone demineralization
Reduce calcium excretion from
Adrenal Gland
Located above kidney suprarenal
gland
Functionally divided into 2 regions
Cortex steroid hormones
Mineralocorticoids
Glucocorticoids
Sex hormones
Medulla neuroendocrine
Epinephrine
Norepinephrine
Regulation
Cortex pituitary (ACTH), Kidney
Adrenal Gland
Enveloped by capsule
Cortex
Mesodermal origin
Functional and
structural zones
Glomerulosa
Fasciculata
Reticularis
Medulla
Neural crest origin
No separate zones
Adrenal gland
1. Capsula
2. Zona
glomerulos
a
3. Zona
fasciculata
4. Zona
reticularis
5. Medulla
6. Medullary
vein
Adrenal gland
Cortex
Capsula
Glomerulosa
Fasci
culat
a
Medulla
Reticularis
Glomerulosa
Outermost
13-15% total volume
Irregular clusters of
small collumnar cell
Little glomeruli
Surrounded by
capillary
Acidophillic
cytoplasm, SER >>,
mitochondria >>
Secrete
mineralocorticoids,
mainly Aldosterone
Glomerulosa
Fasciculata
Middle zone
Largest zone (80%)
Cords of cells
arranged in radial
coloumn (fasciculus)
Polyhedral cells
Rich in lipid
vacuolated
(spongiocytes)
Sinusoidal capllaries
Secrete
glucocorticoids,
mainly cortisol &
Fasciculata
Reticularis
Innermost
Thinnest layer (7%)
Small cells in
branching cords make
reticulum
Small dark
eosinophillic cells
Often has significant
amount of
hemosiderin/lipofuchs
in granules
Secretes androgenic
Reticularis
Medulla
Supported by a
highly vascular
stroma
Cells:
Sympathetic
ganglion cells
Chromaffin or
phaeochrome
cells
H&E stain
SUPRARENAL (ADRENAL)
GLAND
CONSISTS OF
(1) CORTEX
GLUCOCORTICOID:
cortisol, cortisone, corticosterone
MINERALOCORTICOID
ACTH
ADENOHYPOPHYSIS
SUPRARENAL GLAND
(CRF)
HYPOTHALAMUS
STRESS
GLUCOCORTICOID
sympathetic nerve
(2) MEDULLA
(CRF: corticotrophic
releasing factor)
EPINEPHRINE/
NOREPINEPHRINE
BRAINSTEM
FIGHT/FLIGHT
Pancreas
Exocrine & endocrine gland
25 cm long, 5 cm wide, 1-2 cm
thick
Surrounded by fibrous capsule
that form septa
Septa subdivided glands into
lobules
Exocrine : pancreatic acini
Endocrine: islets of
Pancreas
Endocrine Pancreas
Cells form clusters:
pancreatic islets, or islets of
Langerhans
4 Types of Cells in Pancreatic Islets
Alpha cells: produce glucagon, increase
blood glucose level
Beta cells: secrete insulin, antagonist of
glucagon
Delta cells: produce peptide hormone
identical to GH-IH (somatostatin) &
gastrin
F cells: secrete pancreatic polypeptide
Endocrine Pancreas
Islets cells
cell
70%, scattered but concentrated
in the center of islet
Granules :300 nm in diameter
cell
20%, islet periphery
Granules : 250 nm in diameter
cell
1 %, scattered
Granules : 350 nm in diameter
PANCREAS
PANCREAS
EXOCRINE GLAND DIGESTIVE ENZYMES
ENDOCRINE GLAND
ALPHA CELL
GLUCAGON
(ISLETS OF
GLUCOSE
LANGERHANS BETA CELL
INSULIN
GLUCOSE
* somatostatin
F cell : secretes pancreatic polypeptide (regulates the
release of pancreatic digestive enzymes)
Pineal Gland
Epiphysis cerebri
Conical shape, 3-5 mm x
5-8 mm
Surrounded by piamater in
infiltrated to form septa
Function photoreceptor
organ
Secretes melatonin
Neuroendocrine
transducer
Regulates circadian
rhythms
Onset of puberty
Pineal gland
Epiphysis cerebri
Epiphysis cerebri
MOOD
PINEAL GLAND
SEROTONIN
PINEALOCYTES
&
(EPIPHYSIS CEREBRI)
MELATONIN
CIRCADIAN RHYTHM
&
Thank you..
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