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CHROMATOGRAPHY
DEVELOPMENT & APPLICATION
SUBJECT:-FUNDAMENTAL OF BIOPROCESS
ENGINEERING
VIKAS KUMAR
MAYUR SOLANKI
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U13CH006
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U13CH075
INTRODUCTION
The RFC itself was actually invented in the 1950s. Radial
Flow Chromatography (RFC) is an efficient, low pressure
technology for bio-molecule fractionation &
purification. RFC is very suitable for high throughput
adsorptive separations in process.The short bed height,
packed bed, allows robust adsorptive separations from
crude feed (large dirt tolerance)
In an RFC column , the mobile phase flows in the radial
direction, not in the axial direction. The mobile enters from
the outside tube and merges into the center tube .
The radial flow chromatography column consist of two
concentric porous cylindrical frits between which the
stationary phase is packed.
RADIAL
AXIAL
COLUMN CONFIGURATIONS
DEVELOPMEN
In 1947, Hopf (2) described a radial
T device. The device
chromatography
1ST RADIAL FLOW
COLUMN
a.Slurry concentration
b.Packing velocity
c.Amount of settleled resin: suplus or
insufficient
d,D.Viscosity (density) of the packing
buffer
The Picture shows a column being packed. ~85% of the resin is packed
APPLICAT
ION
Protein Bioseparation
flat sheet
hollow fibre
radial flow
Thanks