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CHROMATOGRAPHY
SUBMITTED TO : Dr. MAUSUMI MUKHOPADHYAY
INTRODUCTION
INTRODUCTION
WHAT IS SMBC
In the simulated moving bed technique instead of moving the bed, the feed
inlet, the solvent or eluent inlet and the desired product exit and undesired
product exit positions are moved continuously. giving the impression of a
moving bed, with continuous flow of solid particles
WHAT IS SMBC
This improved economic performance is brought about by a valve-andcolumn arrangement that is used to lengthen the stationary phase
indefinitely and allow very high solute loadings to the process.
ADVANTAGES OF SMBC
SMB provides lower production cost by requiring less column volume, less
chromatographic separation media ("packing" or "stationary phase"), using
less solvent and less energy, and requiring far less labour .
DISADVANTAGES OF SMBC
It is not readily suited for solvent gradients because this kind of purification
may be preferred for the purification of some biomolecules. A continuous
chromatography technique to overcome the two fraction limit and to apply
gradients is multicolumn counter current solvent gradient purification.
Here the solid phase and liquid phase(solvent) moves opposite in the
direction.
This will cause one component to move upward and another to move
downward ,leading thus to separation.
WORKING OF SMB
WORKING OF SMB
Practically counter current operation is achieved by using several fixed bed
column in series .The solids does not actually moves ,its flow is only
simulated by shifting inlet and outlet lines. So this simulated solid flow rate
downward is directly linked to shift period.
The flow rates must be chosen to stabilise the B(more retained product) front
between zones 1 and zone 2 and the A front between zones 3 and 4, and to
allow separation between zones 2 and 3.
Liquid and feed moves in the same direction and solid phase in opposite
direction.
.
WORKING OF SMB
WORKING OF SMB
WORKING OF SMB
APPLICATION OF SMB
Separation of sugars
Desalting
Purification of antibodies
SEPARATION OF SUGARS
The fructose forms a complex with the calcium ions and is retarded
The glucose and other oligosaccharides are eluted with the eluent
SMB packed with cationic resins in calcium form have also been used to
obtain other monosaccharides such as xylose or arabinose.
SMB has received attention for chiral separations since early 1990s and is
today, considered as a cost-effective purification technology and common
to tool for separating isomers at production scale
Both high purity (>99 per cent) and high yield (>99 per cent) were achieved
experimentally using an SMB with a pressure limit of 2.4 Mpa.
Ibuprofen has a chiral centre and therefore exists as S(+) and R(-)
enantiomers.
Many separations of organic molecules have been performed with the SMB
technology like the separation of fatty acids like separation of EPA (C20)and
DHA(C22 methyl esters) from fish oil.
Each outlet (extract and raffinate) was processed using a falling film
evaporator consisting of tubes of 10 mm i.d. For this separation, the
pressure was set to 180 mbar, and the oil temperature to 80C. The
concentrated extract and raffinate were recovered in 50 litre glass vessels.
We get extract purity of 98.4% and to a raffinate purity of 99.4%.
Mitsubishi SP70 is used as the adsorbent for the SMB to separate BDO and PDO.
The adsorption isotherms, the axial dispersions, and the mass transfer coefficients
for BDO and PDO in packed columns with SP70 resin were investigated and
confirmed by fitting the experimental results to the prediction by ASPEN
simulation.
The SMB has four sections, and each has two columns.
The liquid effluent from the fourth section is recycled to the first section, and the
recycling design is called a closed-loop design.
The liquid flow rate in each section of the SMB is controlled by four HPLC
pumps installed at ports of desorbent, feed, extract, and recycle. The movement
of the solid is fulfilled by constantly switching the ports of inlets and outlets to the
next column, and the flow rate of the solid can be controlled by setting the time
period for switching the ports.
Retention times for PDO and BDO are 2.05 and 3.45 min.
Eight PVC columns packed with SP70 for the SMB are used.
In the SMB, each column is installed with a multiport valve and an additional
multiport valve is installed to guide the liquid flow at the port of extract.
A check valve is also installed for each column to prevent the back flow of
liquid when switching the multiport valve to simulate the movement of a solid.
Back pressure regulators, with 50 psi and 250 psi, are installed to stabilize
the flow rate and the pressure of the SMB system.
Various parameters are calculated like dead volume dead time, adsorption
isotherms,mass transfer coefficients by carrying out the displacement
chromatography with HPLC system.
Here , during experiment we are keeping flow rate constant and changing the
time of switching valves.
After the steady state is reached, concentration of BDO and PDO were
calculated and it was observed that experiments conducted with switching
times ranging from 9 to 11 mins can be accounted for as experiments with
separable operating conditions
From the above experiment it was found that optimised separation is 100%
pure BDO and 92.56% pure PDO, the productivity of the SP70 is 0.101 KKD
(kg/kg/day), and the amount of water recycling is 300 L/kg.
REFERENCES
http://dx.doi.org/10.1016/S0149-6395(00)80060-4
http://dx.doi.org/10.1016/j.jtice.2015.12.003
https://en.wikipedia.org/wiki/Simulated_moving_bed
http://sembabio.com/simulated-moving-bed-chromatography