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  • Cell Biology: The Polymerase Chain Reaction (PCR) Technology

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    Ahmed Ali
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    The document discusses the polymerase chain reaction (PCR) technique, which amplifies a specific DNA sequence using a DNA polymerase enzyme. It explains that PCR requires a target DNA sequence, two primers that bind to the DNA, a DNA polymerase, nucleotides, and a thermal cycling machine. The technique works by cycling between high and low temperatures to denature the DNA, anneal the primers, and extend the DNA in an exponential process, doubling the number of DNA copies in each cycle and allowing very small amounts of DNA to be amplified rapidly into billions of copies.

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    PCR

    Original Title

    pcr (1)

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    The document discusses the polymerase chain reaction (PCR) technique, which amplifies a specific DNA sequence using a DNA polymerase enzyme. It explains that PCR requires a target DNA sequence, two primers that bind to the DNA, a DNA polymerase, nucleotides, and a thermal cycling machine. The technique works by cycling between high and low temperatures to denature the DNA, anneal the primers, and extend the DNA in an exponential process, doubling the number of DNA copies in each cycle and allowing very small amounts of DNA to be amplified rapidly into billions of copies.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    11 views11 pages

    Cell Biology: The Polymerase Chain Reaction (PCR) Technology

    Uploaded by

    Ahmed Ali
    The document discusses the polymerase chain reaction (PCR) technique, which amplifies a specific DNA sequence using a DNA polymerase enzyme. It explains that PCR requires a target DNA sequence, two primers that bind to the DNA, a DNA polymerase, nucleotides, and a thermal cycling machine. The technique works by cycling between high and low temperatures to denature the DNA, anneal the primers, and extend the DNA in an exponential process, doubling the number of DNA copies in each cycle and allowing very small amounts of DNA to be amplified rapidly into billions of copies.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 11

    Cell Biology

    The Polymerase Chain Reaction


    (PCR) Technology

    PCR
    Requirements
    1) Target DNA
    2) Two
    oligonucleotide
    primers
    3) DNA polymerase,
    4) dNTPs
    5) PCR machine

    Biochemistry of the PCR

    PCR
    Requirements
    1) Target DNA
    2) Two
    oligonucleotide
    primers
    3) DNA polymerase,
    4) dNTPs
    5) PCR machine

    Cell Biology

    PCR Animation

    Cell Biology

    PCR Continued

    PCR
    Now

    Then

    PCR
    The Cycles

    PCR

    PCR
    Exponential Amplification
    Cycles
    1
    2
    4
    10
    15
    20
    25
    30

    Copies
    2
    4
    16
    1,024
    32,768
    1,048,576
    33,554,432
    1,073,741,824

    PCR
    Exponential
    Amplification

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