Journal Reading

The wound healing effects of vitamin A eye

drops after a corneal alkali burn in rats

Pembimbing:
Dr. Margrette. P. Franciscus. Sp.M MSc
Oleh:
Megala a/p Bala Krishnan
112014204

Introduction

 Vascular endothelial growth factor A

(VEGF-A) , Basic fibroblast growth
factor (bFGF) and transforming
growth factor (TGF)-a and b
promotes division and proliferation
of endothelial cells
Thrombospondin 2 (TSP2) repress
new vessels growth

 Vitamin A is necessary for the normal

growth and differentiation of
epithelium
may accelerate the reepithelialization and also inhibit
VEGF-A

Material and Methods

Animals:
160 Sprague-Dawley male rats (weight
range, 250 300 g) divided into 2 groups
Deeply anesthetized by an
Intraperitoneal injection

 The central cornea of the right eye

was injured by placing a filter paper
(circular 3.0 mm diameter) saturated
with 0.01 M NaOH on it for 45
seconds.
The wound surface was then washed
with 0.9% physiological saline.

The vitamin A group was treated with aqueous ophthalmic

T

 Wound sizes were determined by

staining with 1% fluorescein and
photographing immediately after
injury and at 24, 48 and 72 hr after
injury.
The percentage of wound healing
was calculated
using the following
(initial wound area- wound
formula area)/initial wound area x 100.

Impression cytology of bulbar

conjunctiva

Protein extraction and ELISA for VEGFA, thrombospondin 2, MMP 9 and TGF-b
Corneas were

Results
Effect of vitamin A eye drops on
corneal epithelial wound healing
Percentage of wound healing higher
No corneal opacity observed
VEGF-A, (bFGF) and (TGF) lower but TSP 2 is
higher

Discussion
This study is the first to show that
vitamin A eye drops can promote
wound healing by activating
thrombospondin 2 and inhibiting
VEGFA, MMP 9 and TGF-b following a
corneal alkali burn in rats