Gene Therapy Vector:

Bacteria via
MacrophagePresented by:

Fenny Aulia Sugiana

Janrigo Klaumegio
Mere

Dosen Pengampu: Dr. Djarot Sasongko

 Construct Plasmid

Therapeutic gene
that give cytotoxic
effect on tumor
cells (Apoptosis)
Additionally induced
gene expression in
response to hypoxia
via HRE sequences.
Gene for human cancer
therapy
Types of Bacteria Used
 bacteri
Overview of a
Disable
process bacteria

This is a diagram
of Gene Therapy
 What is it?
The Technique using bacteria for direct gene
transfer into the tissue, organ, or organism to
treat diseases.
This is also called Bactofection
Bacteria are used as vehicles or vectors to
transport the genetic info into the cell
 Step 1
Bacteria is entered into cell through endocytosis
(eating)
Transformed bacteria that contain plasmids
carrying the transgene are entered into the cell
and enters cytoplasm
The
Genetically engineered
vectors also provide bacteria penetrate into
protection
the cells to the genetic
cargo and prompt
compartmental escape
as the endosome
gradually acidifies.
 Step 2
Once in cell, the bacteria undergo lysis
and are destructed when they enter the
cytoplasm
From b to c, the bacteria disappears
 Step 3

They release the DNA plasmids

Released plasmids then enter the nucleus

The transgene is expressed by the

transcription and translation machinery
 Step 4
The transgene product can be expressed :

The intercellular bacteria can produce and deliver

RNAs and Proteins
 Step 5

The cell is then changed or corrected

Then, the corrected proteins are

produced
Microbe & Macrophage
Advantages/Disadvanta
ges
Low cost Not safe
The immune system
Simple production/ delivery could react and
cause clearance of
A lot of DNA is delivered bacteria
Not efficient
Possible side
effects
Bacterial strains can
acquire virulence
factors back and
could lead to
serious infections
 Bibliography
http://www.nature.com/mt/journal/v17/n5/f
ull/mt200941a.html Molecular Therapy
(2009) Yiqi Seow and Matthew J. Wood
http://nanosingularity.wordpress.com/2007
/07/07/bacteria-ferry-nanoparticles-into-
cells-for-early-diagnosis-treatment/
Bacteria ferry nanoparticles into cells for
early diagnosis, treatment . July 7, 2007