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Michaelis-Menten Kinetics
Variables Parameters
[E]: free enzyme molecules kf , kr , kcat : reaction rates
[S]: free substrate molecules
[ES]: enzyme-substrate complexes
[P]: free product molecules
Writing Word Equations
A word equation is a qualitative description
of the systems major processes
# of free S # of ES
# of free S # of free S
molecules complexes
molecules = molecules binding to + splitting
at time t +1 at time t
free E into E + S
# of ES complexes
splitting into E + S = kr [ES]t
conc. of free enzyme
conc. of free substrate
# of free S molecules
binding to free E = kf [E]t [S]t
by Law of Mass Action
Formal Equations for
Enzyme Kinetics Model
nonthreatening to students
Estimate vmax
for this enzyme.
Further Exploration
Biological interpretation of KM?
Nonlinear regression:
Fit the appropriate
curve to the M-M plot
by minimizing the sum
of squared residuals.
Example
Lineweaver-Burk plot:
1/v vs. 1/[S]0
Formula = ?
Each methods
sensitivity to
estimation error?
Each methods
ability to detect
such error?
Beyond Simple Enzyme Kinetics
Simulate &
visualize behavior
of each indiv.
molecule
Focus of next
weeks seminar
(Nernst equation)
Take-home message