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  • Enterobakteria

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    Muhammad Mirza
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    1. Enterobacteriaceae are a family of Gram-negative, non-spore forming, facultatively anaerobic bacteria commonly found in the intestines of humans and animals. 2. Key characteristics include rod-shaped morphology, fermentative metabolism of glucose producing acids, and the ability to reduce nitrates. Identification is based on morphology, Gram stain reaction, growth characteristics, and biochemical profiles. 3. The family includes many important enteric pathogens that can cause diseases like salmonellosis, shigellosis, and various forms of diarrhea. Common identification tests involve analyzing reactions on triple sugar iron agar, and in assays for properties such as indole production, citrate use, and mot

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    1. Enterobacteriaceae are a family of Gram-negative, non-spore forming, facultatively anaerobic bacteria commonly found in the intestines of humans and animals. 2. Key characteristics include rod-shaped morphology, fermentative metabolism of glucose producing acids, and the ability to reduce nitrates. Identification is based on morphology, Gram stain reaction, growth characteristics, and biochemical profiles. 3. The family includes many important enteric pathogens that can cause diseases like salmonellosis, shigellosis, and various forms of diarrhea. Common identification tests involve analyzing reactions on triple sugar iron agar, and in assays for properties such as indole production, citrate use, and mot

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    165 views41 pages

    Enterobakteria

    Uploaded by

    Muhammad Mirza
    1. Enterobacteriaceae are a family of Gram-negative, non-spore forming, facultatively anaerobic bacteria commonly found in the intestines of humans and animals. 2. Key characteristics include rod-shaped morphology, fermentative metabolism of glucose producing acids, and the ability to reduce nitrates. Identification is based on morphology, Gram stain reaction, growth characteristics, and biochemical profiles. 3. The family includes many important enteric pathogens that can cause diseases like salmonellosis, shigellosis, and various forms of diarrhea. Common identification tests involve analyzing reactions on triple sugar iron agar, and in assays for properties such as indole production, citrate use, and mot

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    Enterobacteriaceae

    Wilda Mahdani
    Mikrobiologi Klinik FK-USK, RSUDZA
    Famili Enterobacteriaceae
    Batang Gram negatif, tidak berspora, fakultatif
    anaerob
    Free living : tanah, air
    buah2an, sayuran, biji2an,
    tumbuhan, cacing, serangga, (usus)
    hewan danmanusia
    Famili Enterobacteriaceae
    Morfologi : 0,3 1,8 u, single, flagella + / -
    Pertumbuhan : suhu optimum 37oC
    Biokimia : fermentatif / respiratif
    memecah glukosa
    mereduksi nitrat
    oksidase -, katalase +
    Famili Enterobacteriaceae
    Diferensiasi dari famili lain didasarkan pada :

    * morfologi : ukuran, bentuk (rounded


    end), flagella (peritrichous)
    * pewarnaan Gram
    * pertumbuhan : koloni, kebutuhan nutrisi
    * sifat biokimia
    * patogenitas
    Klassifikasi Bergey (1974)
    Tribe I Escherichieae : Escherichia, Shiegella,
    Edwardsiella, Salmonella, Citrobacter
    Tribe II Klebsiella : Klebsiella, Enterobacter,
    Hafnia, Serratia
    Tribe III Proteae : Proteus, Morganella,
    Providencia
    Tribe IV Yersinieae : Yersinia
    Tribe V Erwinieae : Erwinia
    Pembagian Famili Enterobacteriaceae
    berdasarkan kemampuan meragi laktosa :

    Lactose Fermenters

    Nonlactose Fermenters
    Mac Conkey Media
    Pembagian Enterobacteriaceae :

    True pathogen Enterobacteriaceae

    Opportunistic Enterobacteriaceae
    ENTERIC PATHOGEN : Enterobacteriaceae
    Gastrointestinal disease

    Salmonella

    Shigella

    Diarrheagenic Escherichia coli

    Yersinia
    SIFAT BIOKIMIA :

    Produksi energi :
    Fermentasi karbohidrat (Anaerob / O2 )
    Siklus TCA & sistem transpor elektron (O2 cukup)
    Meragi glukosa asam (mixed acid pathway), kecuali
    Klebsiella (Butanediol fermentative pathway)
    Reduksi nitrat nitrit
    PERBENIHAN :

    Media nondiferensial / non selektif : tidak dapat


    dibedakan

    Koloni : moist, smooth rough, gray

    Hemolisa - / + (tipe )

    Media diferensial & selektif


    MEDIA UNTUK ISOLASI KUMAN ENTERIK

    Media diferensial :
    Mac Conkey agar
    Eosin Methylene Blue agar (EMB)
    Desoxycholate agar

    Media selektif :
    Salmonella Shigella agar
    Desoxycholate citrate agar
    Hektoen enteric agar
    Xylose-lysine desoxycholate agar
    Salmonella Shigella Agar (SS)
    DAYA TAHAN :

    Rusak :
    Panas
    Germisida & Desinfektan [ ]
    Bakterisidal :
    Phenol
    Formaldehid
    -glutaraldehid
    Halogen
    Bakteriostatik :
    Quaternary ammonium compound
    PENYAKIT

    UTI

    Wound infections

    Pneumonia

    Meningitis

    Sepsis

    Beberapa gangguan gastrointestinal


    Identifikasi
    Media TSI (Triple Sugar Iron) agar

    Uji biokimia :
    Indole
    Methyl red
    Voges-Prouskauer reaction
    Urea
    Simmons Citrate agar
    Identification

    Triple sugar iron agar

    0.1% glucose
    1% lactose
    1% sucrose
    Ferrous sulfate
    pH indicator: phenol red
    Triple Sugar Iron
    Agar
    TSI Media
    Indol Test
    Indole Test
    Methyl Red/Voges Proskauer (MR/VP)
    Media dan Reagen
    Glucose Broth
    Methyl Red indicator for acid
    Voges Proskauer reagentsA: 5% Alpha-Naphthol, &
    ethanol, B: Potassium Hydroxide, & Deionized Water.
    Cara inokulasi kuman pada 2 media cair
    glukosa. Inkubasi 48 jam. Tetes masing2 MR dan VP
    MRtests for acid end products from glucose
    fermentation.
    VPtests for acetoin production from glucose
    fermentation.
    MR/VP continued
    Reading Results:
    MR a + result is red (indicating pH below 6) and a result is yellow
    (indicating no acid production)
    VPA + result is red after VP reagents are added (indicating the presence of
    acetoin) and a result is no color change.

    Methyl Red: left and right + VP: left + and right


    MR Test
    VP test
    Motility
    Deteksi pergerakan bakteri

    Media semisolid

    Indikator Tetrazolium (TTC)

    Tusuk media semisolid dengan needle


    Motility

    From left to right:


    + +
    Motility Medium
    Citrate

    Cara inokulasi kuman pada slant


    Media and Reagents Used: Simmons Citrate Agar
    contains sodium citrate (carbon source), ammonium
    ion (nitrogen source), & pH indicatorbromthymol
    blue.
    Hasil
    Bila kuman dapat memetabolisme sitrat, maka
    media akan menjadi biru
    Citrate

    Left positive and right negative.


    Simmons Citrate Agar Media
    Urease Test

    H2O
    Urea CO2 + NH3 NH4+ + OH-
    Enterotube
    Microbact
    Enterobacteriaceae
    E.coli
    Enterobacteriaceae

    Klebsiella pneumoniae
    Enterobacteriaceae

    Serratia marcescens
    Salmonella
    Enterobacteriaceae

    Proteus mirabilis

    Profil Biokimia

    Koloni
    Sekian

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