3 ml reagen 0,3 ml serum

CARA KERJA
Vortex

diamkan selama 1 menit

Baca absorbansi spektrofotometer (=340 nm)

Hidupkan stopwatch

Baca absorbansi lagi setelah 1 menit, 2 menit, dan 3 menit

ASAT activity (U/L)= A/min sample x 1745

 DISKUSI
Apa yang anda ketahui tentang AST?
Apakah fungsi AST bagi metabolisme didalam
tubuh?
Mengapa AST bukan indikator yang spesifik bagi
Liver?
REAGENT COMPOSITION
R1:1. TRIS (pH=7,65) 110 mmol/L
2. L-Aspartate 320 mmol/L
3. Malate Dehydrogenase (MDH) 800 U/L
4. Lactate Dehydrogenase (LDH) 1200 U/L
R2:1. 2-Oxoglutarate 65 mmol/L
2. NADH 1 mmol/L
 TEST PRINCIPLE
2-Oxoglutarate L-Aspartate

AST/GOT

L-Glutamate Oxaloacetate NADH+ H+

MDH

L-Malate NAD+

NADH is oxidized to NAD+, the resulting decrease in absorbance at 340

nm is directly proportional to the activity of GOT in the sample.
This is a modified formulation for the assay of GOT, as recommended by
the IFCC (International Federation of Clinical Chemistry).
 CALCULATION
GOT (IU/L) = A/min x Factor
Substrate Start: 25 or 30C 37C
Factor at 340 nm 1151 2143
Factor at 334 nm 1173 2184
Factor at 365 nm 2132 3971
Sample Start: 25 or 30C 37C
Factor at 340 nm 952 1745
Factor at 334 nm 971 1780
Factor at 365 nm 1765 3235
REFERENCE RANGE *(IU/L)

25C 30C 37C

males <18 <25 <37

females <15 <21 <31

 Aspartate Transaminase
(SGOT)
AST is called serum glutamic
oxaloacetic transaminase (SGOT).
Aspartate aminotransferase (AST, EC
2.6.1.1) is enzymes found mainly in
the liver, but also found in red blood
cells, heart cells, muscle tissue and
other organs, such as the pancreas
and kidneys.
1 = Malate dehydrogenase
2 = Aspartate aminotransferase
 Aspartate Transaminase (AST), also known as serum
glutamic oxaloacetic transaminase (GOT) or aspartate
aminotransferase (ASAT/AAT), facilitates the conversion of
aspartate and -ketoglutarate to oxaloacetate and
glutamate.
There are two isoenzymes in humans:
GOT1 is a cytosolic isoenzyme derived from red blood cells and
heart;
GOT2 is the mitochondrial isoenzyme found mainly in the liver.
AST is elevated in liver and muscle diseases. It is part of
diagnostic tests for liver function, myocardial infarction,
acute pancreatitis, acute hemolytic anemia, severe burns,
acute renal
1 = Malate disease and trauma.
dehydrogenase
2 = Aspartate aminotransferase
1 = Malate dehydrogenase
2 = Aspartate aminotransferase