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Integenx RapidHIT
System
Molly Yesho
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RapidHIT System Overview

 Fully automated

 STR based DNA profiles

 Results within 2 hours

 Profiles uploaded to CODIS

 NDIS approved

 Can be performed outside a lab

https://integenx.com/rapidhit-system/
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Using the Integenx RapidHIT
System
 Disposable GlobalFiler Express Cartridges loaded

 Samples loaded into cartridge

 Push “run”

 “Gold Standard” Sample Extraction

 PCR Based Amplification

 Capillary Electrophoresis Separation


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PCR in RapidHIT System

 Can use PowerPlex 16, GlobalFiler Express, or AmpFlSTR

 PCR for GlobalFiler Express


 Pre-incubation temperature: 96°C
 Denaturation temperature: 96°C
 Annealing temperature: 60°C
 Final Extension: 8 minutes
 28 PCR Cycles
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https://www.slideshare.net/wally1727/rapid-hit-presentation-for-forensics-labs-june2012
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GlobalFiler Express Cartridge

https://www.slideshare.net/wally1727/rapid-hit-presentation-for-forensics-labs-june2012
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GlobalFiler Express Cartridge

https://www.slideshare.net/wally1727/rapid-hit-presentation-for-forensics-labs-june2012
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GlobalFiler Express Cartridge

https://www.slideshare.net/wally1727/rapid-hit-presentation-for-forensics-labs-june2012
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Results
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Advantage or Disadvantage?

 Doesn’t require a DNA analyst, or a laboratory setting

 Minimal training for operation

 Automation

http://www.marshall.edu/wamnewsletter/files/2013/09/RapidDNAPhoto1.jpg
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Advantages

 DNA analysis brought to new markets


 Property Crime
 Immigration
 Department of Defense
 Mass Causalities

 Obtain DNA profiles faster


 2 hours compared to average time
of 106 days
 Can help stop repeat offenders

https://integenx.com/wp-content/uploads/D007780-RapidHIT-Brochure-The-Case-
for-Rapid-DNA.pdf
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Rapid DNA Act of 2017

 Signed in August of 2017

 Now allows use of RapidHIT systems in booking process

 Helps to quickly identify or exonerate suspects

https://www.govtrack.us/congress/bills/115/s139
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References

 RapidHITSystem. Webinar: Using RapidHIT and


GlobalFiler Express Kit for CODIS Upload https://
www.youtube.com/watch?v=WxqA8E8KYJg (accessed
Nov 9, 2017).

 Integenx. RapidHIT System. https://integenx.com/


rapidhit-system/ (accessed Oct 28, 2017).

 Butler, John M. Fundamentals of Forensic DNA Typing, 1st ed.;


Academic Press: San Diego, 2010.
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QUESTIONS?

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