The document summarizes hematopoiesis, the formation of blood cells. It describes how blood cells are formed in different locations throughout development, with the yolk sac and liver playing roles early on and bone marrow becoming the primary site of hematopoiesis in the third trimester. The principal types of blood cells - red blood cells, white blood cells, and platelets - are formed in the bone marrow. White blood cells are further classified and their maturation processes outlined.
The document summarizes hematopoiesis, the formation of blood cells. It describes how blood cells are formed in different locations throughout development, with the yolk sac and liver playing roles early on and bone marrow becoming the primary site of hematopoiesis in the third trimester. The principal types of blood cells - red blood cells, white blood cells, and platelets - are formed in the bone marrow. White blood cells are further classified and their maturation processes outlined.
The document summarizes hematopoiesis, the formation of blood cells. It describes how blood cells are formed in different locations throughout development, with the yolk sac and liver playing roles early on and bone marrow becoming the primary site of hematopoiesis in the third trimester. The principal types of blood cells - red blood cells, white blood cells, and platelets - are formed in the bone marrow. White blood cells are further classified and their maturation processes outlined.
By: Zelalem A. Hematopoiesis /Hemopoiesis It is the formation and development of blood cells.
In the earliest phase of human embryogenesis,
blood cells arise from the yolk sac mesoderm.
In the second trimester, hemopoiesis (also called
hematopoiesis) occurs primarily in the developing liver, with the spleen also playing a role. Zelalem A. @ DTU 4/30/2018 2 Skeletalelements begin to ossify and bone marrow develops in their medullary cavities, so that, in the third trimester, bone marrow increasingly becomes the major hemopoietic organ.
Zelalem A. @ DTU 4/30/2018 3
The principal hematopoietic organ is the bone marrow, where:
The red blood cells (erythrocytes)
Granular white blood cells (granular leukocytes),
and certain agranular white blood cells (lymphocytes) and
Blood platelets (thrombocytes) are formed.
Zelalem A. @ DTU 4/30/2018 4
Itis mostly lymphocytes that are formed in the other hematopoietic organs. that is:
The lymph nodes
Spleen and Thymus
Zelalem A. @ DTU 4/30/2018 5
Zelalem A. @ DTU 4/30/2018 6 Zelalem A. @ DTU 4/30/2018 7 Blood- special type of connective tissue Has two components 1. Formed elements (45%) - the actual cellular components of blood a. Erythrocytes (Red blood cells) b. Leukocytes (White blood cells) c. Thrombocytes (Platelets ) 2. Plasma (55%) - is the fluid portion of the blood.
Zelalem A. @ DTU 4/30/2018 8
Zelalem A. @ DTU 4/30/2018 9 Erythrocytes (red blood cells) are terminally differentiated, lack nuclei, and are packed with the O2-carrying protein hemoglobin.
Under normal conditions, these corpuscles never
leave the circulatory system.
They are approximately 7.5 m in diameter, 2.6 m
thick at the rim, and only 0.75 m thick in the center.
This biconcave shape provides a large surface-to-
volume ratio and facilitates gas exchange. Zelalem A. @ DTU 4/30/2018 10 Zelalem A. @ DTU 4/30/2018 11 Erythrocyte maturation involves hemoglobin synthesis and formation of a small, enucleated, biconcave corpuscle. Several major changes take place during erythrocyte maturation.
Cell and nuclear volume decrease, and the
nucleoli diminish in size and disappear.
The chromatin becomes increasingly denser until
the nucleus presents a pyknotic appearance and is finally extruded from the cell.
Zelalem A. @ DTU 4/30/2018 12
There is a gradual decrease in the number of polyribosome (basophilia decreases), with a simultaneous increase in the amount of hemoglobin (an acidophilic protein) within the cytoplasm.
Mitochondria and other organelles gradually
disappear.
Zelalem A. @ DTU 4/30/2018 13
Zelalem A. @ DTU 4/30/2018 14 Leukocytes(white blood cells) migrate to the tissues where they become functional and perform various activities.
According to the type of cytoplasmic
granules and the shape of their nuclei, leukocytes are divided into two groups:
polymorphonuclear granulocytes and
mononuclear agranulocytes.
Zelalem A. @ DTU 4/30/2018 15
Zelalem A. @ DTU 4/30/2018 16 Granulocytes possess two types of granules:
1. The specific granules that bind neutral, basic,
or acidic stains and have specific functions and
1. The azurophilic granules, which are
specialized lysosomes, stain darkly, and are present at some level in all leukocytes.
Granulocytes have polymorphic nuclei with
two or more lobes
Zelalem A. @ DTU 4/30/2018 17
Zelalem A. @ DTU 4/30/2018 18 Agranulocytes do not have specific granules, but they do contain azurophilic granules (lysosomes).
Zelalem A. @ DTU 4/30/2018 19
constitute 60–70% of circulating leukocytes.
They are 12–15 m in diameter in blood
smears, with nuclei having two to five lobes linked by thin nuclear extensions.
Neutrophils are inactive and spherical while
circulating but become actively amoeboid during diapedesis and upon adhering to solid substrates such as collagen in the ECM.
Zelalem A. @ DTU 4/30/2018 20
Zelalem A. @ DTU 4/30/2018 21 Eosinophils are far less numerous than neutrophils, constituting only 2–4% of leukocytes in normal blood. Characterized by bilobed nucleus
Zelalem A. @ DTU 4/30/2018 22
Basophils are also about 12–15 m in diameter, but make up less than 1% of blood leukocytes and are therefore difficult to find in smears of normal blood.
The nucleus is divided into two or more
irregular lobes, but the large specific granules overlying the nucleus usually obscure its shape.
Zelalem A. @ DTU 4/30/2018 23
Zelalem A. @ DTU 4/30/2018 24 Lymphocytes constitute a family of leukocytes with spherical nuclei
They can be subdivided into functional
groups according to distinctive surface molecules (markers) that can best be distinguished immunocytochemically, notably
T lymphocytes, B lymphocytes, and Natural killer (NK) cells.
Zelalem A. @ DTU 4/30/2018 25
Lymphocytes vary in life span according to their specific functions;
Some live only a few days and others survive
in the circulating blood or other tissues for many years.
They are the only type of leukocytes that,
following diapedesis, can return from the tissues back to the blood.
Zelalem A. @ DTU 4/30/2018 26
circulating lymphocytes originate mainly in the thymus and the peripheral lymphoid organs (eg, spleen, lymph nodes, tonsils etc.).
However, all lymphocyte progenitor cells
originate in the bone marrow.
Some of these lymphocytes migrate to the
thymus, where they acquire the full attributes of T lymphocytes. Zelalem A. @ DTU 4/30/2018 27 Other bone marrow lymphocytes differentiate into B lymphocytes in the bone marrow and then migrate to peripheral lymphoid organs, where they inhabit and multiply in their own special compartments.
Zelalem A. @ DTU 4/30/2018 28
Zelalem A. @ DTU 4/30/2018 29 Monocytes are bone marrow–derived agranulocytes with diameters varying from 12 to 20 m.
The nucleus is large, off-center, and may be
oval, kidney-shaped, or distinctly U-shaped
Zelalem A. @ DTU 4/30/2018 30
Mature monocytes enter the bloodstream, circulate for about eight hours, and then enter tissues where they mature as macrophages (or other phagocytic cells) and function for several months.
Zelalem A. @ DTU 4/30/2018 31
Circulating monocytes are precursor cells of the mononuclear phagocyte system.
After crossing the walls of postcapillary
venules, monocytes differentiate into macrophages in connective tissues, microglia in the CNS, osteoclasts in bone, etc.
Zelalem A. @ DTU 4/30/2018 32
The monoblast is a committed progenitor cell that is virtually identical to the myeloblast in its morphologic characteristics.
Further differentiation leads to the
promonocyte, a large cell (up to 18 m in diameter) with basophilic cytoplasm and a large, slightly indented nucleus.
Promonocytes divide twice as they develop
into monocytes Zelalem A. @ DTU 4/30/2018 33 Zelalem A. @ DTU 4/30/2018 34 Blood platelets (thrombocytes) are nonnucleated, disklike cell fragments 2–4 m in diameter.
Plateletspromote blood clotting and help
repair minor tears or leaks in the walls of blood vessels, preventing loss of blood.
Normal platelet counts range from 200,000
to 400,000 per microliter of blood and they have a life span of about 10 days. Zelalem A. @ DTU 4/30/2018 35 In stained blood smears, platelets often appear in clumps. Each platelet has a lightly stained peripheral zone, the hyalomere
And a central zone containing darker-staining
granules, called the granulomere
Zelalem A. @ DTU 4/30/2018 36
Zelalem A. @ DTU 4/30/2018 37 plateletsoriginate in the red bone marrow by dissociating from mature megakaryocytes (Gr. megas, big, + karyon, nucleus, + kytos), which in turn differentiate from megakaryoblasts in a process driven by thrombopoietin.