STAINING

TECHNIQUES

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Because microbial
cytoplasm is usually
transparent, it is necessary
to stain microorganisms
before they can be viewed
with the microscope.
BASIC DYE

ACIDIC DYE

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 STAIN

Staining can be performed with The differential stain technique A special stain technique is used
a single stain. distinguishes two kinds of to examine bacterial spores
organisms.
SIMPLE POSITIVE STAINING- crystal
violet or methylene blue

SIMPLE NEGATIVE STAINING- nigrosin or

Congo red
and ears;
• For visualization of morphological shape and arrangement of microorganisms
• To highlight the total count of bacteria
• Ex. Loeffler’s Methylene Blue, Dilute Carbol Fuschin, Crystal Violet
 Gram Staining
This procedure separates bacteria into two
groups: Gram positive bacteria and
Gram negative bacteria.

Gram negative bacteria give

pink color
Gram positive bacteria give
purple color

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 PROCEDURE

Come In And Stain

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This technique differentiates species of Mycobacterium
from other bacteria. Because the cell wall is resistant to
water-based stains, acid-fast organisms require a
special staining technique

acid fast bacteria appear bright red

non-acid fast bacteria appear blue

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SPORE STAIN

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CAPSULE STAIN

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FLAGELLA STAIN

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OTHER STAINING
TECHNIQUES

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 Wheatley Positive Staining Negative Staining Gomori Methenamine Periodic acid-Schiff
Trachoma Stain of Viruses of Viruses Silver (GMS) Staining (PAS ) Staining

Ex. Wheatley’s
stain for Chemical: Chemical: Ex. GMS staining Ex. Candida on
intestinal Uranyl Acetate Uranyl Acetate of Aspergillus PAS Stain
amoeba
References

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THANK YOU