BC-3000 Plus Service Training V2.0 en

BC-3000 plus Service Training
Hematology Global Technical Support Department
service@mindray.com
Version: 2.0
No.：MXQ-12038-BC-3000plus
© 2011 Mindray Confidential
Contents
Principle
1.1 Basic Knowledge
1.2 Principle
BC-3000Plus
2.1 Installation
2.2 Operation
2.3 Liquid System
2.4 Hardware
2.5 System Structure
2.6 Trouble Shooting
2.7 Maintenance
2 © 2011 Mindray Confidential

1. Principle
1.1 Basic Knowledge
1.2 Principle

1.1 Basic Knowledge
Blood Cell Leukocyte Erythrocyte Platelet

Blood
 Blood sample consists of two parts.

1.1 Basic Knowledge
Blood Leukocyte Erythrocyte Platelet
Blood Cell
 There are three kinds of blood cells.
Leukocyte Erythrocyte Platelet

Size: 30-400fl 30-150fl 2-25fl
9 12 9
Amount: 4-10X10 /L 3.5-5.5X10 /L 100-300X10 /L
Blood Blood Cell Erythrocyte Platelet
Leukocyte
Granulocyte
WBC
Defend the body
against infectious
disease and foreign
materials

1.1 Basic Knowledge
Blood Blood Cell Erythrocyte Platelet

Leukocyte
Lymph Baso Mono Eosino

Neutro
Lymphocyte Mid-sized Cell Neutrophilic Granulocyte

1.1 Basic Knowledge
Blood Blood Cell Leukocyte Platelet

Erythrocyte
 The function of RBC is almost completed by hemoglobin (HGB).
Transport oxygen and carbon dioxide

1.1 Basic Knowledge
Blood Blood Cell Leukocyte Erythrocyte

Platelet
Coagulation or clotting

1.2 Principle

1.2 Principle
Electrical Impedance Volumetric Metering Colorimetric Method Reagents
Method
 WBC, RBC and PLT are counted and sorted by the Electrical Impedance Method,
just Coulter Principle. This method is based on the measurement of changes in
electrical impedance produced by a particle passing through an aperture.

1.2 Principle
Method

1.2 Principle
Method
The amplitude of each pulse is proportional to the size of each blood cell.
1.2 Principle
Method
Pulse Graph
1、blood cell of Same size, go through the

aperture by constant speed.
2、blood cell of Same size, go through the

aperture by variable speed.
3、blood cell of different size, go through

the aperture by variable speed.

1.2 Principle
Method

1.2 Principle
Method
 From Pulse Graph to Histogram

1.2 Principle
Method
From Pulse Graph to Histogram

1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents
 This analyzer uses a volumetric metering unit to control the count cycle and
to ensure that a precise volume of sample is analyzed.
Upper sensor Upper sensor
Lower sensor Lower sensor
1 Empty when start 2 Liquid surface fall down through the

volumeritc tube
Upper sensor Upper sensor
Lower sensor Lower sensor
3 Counting start when the 4 Counting end when the liquid

liquid surface pass the upper surface pass the lower sensor
sensor.

1.2 Principle
Method Volumetric Metering Reagents
Colorimetric Method
 HGB is determined by the colorimetric method.

 Lyse will release HGB when RBC was broken down and react with
HGB to generate a mixture for HGB measurement.
 The mixture has the biggest absorbance at 540nm.
I0 It

1.2 Principle
Method Volumetric Metering Reagents
Colorimetric Method
Light Absorbing Detector Signal

source container processing
Display
LED light Bath Photosensor

1.2 Principle
 Reagents are one part of the system.

 Lyse
Break down RBC;
Release HGB and react with HGB to generate a mixture for HGB
measurement;
Help the differential of WBC.
 Diluent
Dilute the blood sample;
Provide a conductive environment for counting and an
environment which is similar to the plasma to maintain the size of
blood cell;
Clean the tube and bath.

1.2 Principle
 Rinse
Clean the bath and metering tube.
 E-Z cleanser
An enzyme-based cleaning solution used to clean the tube and bath.
 Probe cleanser
An alkaline cleaning solution used to clean the tube and bath.

2.1 Installation
Installation Requirements 2.1.1 Installation Requirements

2.1.2 Interface
Open box, check
2.1.3 Installation Procedures
Install
Performance validation

2.1.1 Installation Requirements
Environment Space Power
 Operating temperature: 15 ℃ to 30 ℃.
 Relative humidity: 30% to 85%.
 Atmospheric pressure: 70 kPa to 106 kPa.
 free as possible from dust, mechanical vibrations, loud noises,

and electrical interference.
 Do not place near brush-type motors, flickering fluorescent lights,

and electrical contacts that regularly open and close.
 Do not place in direct sunlight or in front of a source of heat or

drafts.

 At least 40 cm on each side, which is the preferred access to

perform service procedures
 At least 10 cm behind for cabling and ventilation
 There is enough room on or below the countertop to accommodate

the diluent, rinse and waste containers.
(reagents bottle better be the same level of the machine).

 Check the availability of a power outlet that is

 a female receptacle;
 single phase with ground (confirmed third-wire earth ground);
 voltage of zero curve to ground should be ≤5V
 voltage: 100 VAC - 240 VAC
 frequency: 50/60±1 Hz
 fuse: 250V T4A
 Properly grounded socket or connect the grounding pole at the back of
the analyzer to the ground.

2.1.2 Interface
 Front panel
1 ---- Display Screen
2 ---- Keypad
3 ---- Recorder
4 ---- Power Indicator
5 ---- Aspirate Key
6 ---- Sample Probe

2.1.2 Interface
 Rear panel 1 --- RS-232C Serial Port 1
2 --- Parallel Port
3 --- RS-232C Serial Port 2
4 --- Keyboard Interface
5 --- FDD-POWER
7 --- Diluent
8--- Diluent sensor connector
9 --- Rinse sensor connector
10 --- Waste
11 --- Rinse
12 --- Power
13 --- Grounding Pole
Printer:
1.EPSON LX-300/300+ ,
2. EPSON LQ-300K+II, LQ630K
3. EPSON Stylus C-87 , Panasonic KX-P1150
Scanner: TYSSO CCD-82, AIDA8400
Fuse

Release the sample probe Step2 Step3 Step4
2
1
Open the right side door Open the front panel
3 4
Cut the plastic cable tie and get rid of it Move the sample probe to the middle position
Step1 Reagents connection Step3 Step4

Step1 Step2 Install Recorder Paper Step4
1 Open the door of the recorder. 2 Uplift the bar of the recorder. 3 Close the door.
Insert the paper into the paper entry.
Note: The thermo-side should faces up.

Step1 Step2 Step3 Performance Validation
Start the machine
Check background
Do QC test,
check repeatability
Test fresh blood sample
Any step has problem,

please check the machine

2.2 Operation
N
Repeatability good
2.2.1 Count Screen
Y
Y N 2.2.2 Menu
Calibration Troubleshooting 2.2.3 Startup&background
2.2.4 Daily Quality Control
2.2.5 Repeatability test
2.2.6 Calibration
2.2.7 Compare
2.2.8 Parameters

2.2.1 Count Screen
 Main Screen
Error message
area Status area
Title
area
Analysis area
Menu area
Help area

2.2.2 Menu
If you want to…

analyze samples
select an appropriate analysis mode
review sample results
run the QC program
customize system software
maintain/service the analyzer
calibrate the analyzer
look for help
shut down the analyzer

2.2.3 Startup & background
 After powering on the instrument, the power indicator will light on

and the screen will display “Initializing…”.
 The analyzer will sequentially initialize the file, hardware and
fluidic systems.
 The whole initializing process lasts about 3 - 5 minutes, depending
on how the analyzer was previously shut down.
Parameter Background Range

WBC  0.3  109 / L
 Background Test RBC  0.03  1012 / L

HGB 1g/L
HCT  0.5 %
PLT  10  109 / L

2.2.4 Daily Quality Control Test
 Daily work: Before running any samples, use Quality Control or

Fresh Blood to check the stability of instrument.

2.2.5 Repeatability Test
 Good repetition, poor accuracy

 8.9, 9.0, 8.9, 8.9, 8.8 (reference value: 9.6+0.5)
Recalibrate
 Poor repetition, poor accuracy
 8.5, 9.4, 10.1, 8.0, 10.5 Troubleshooting
 Good repetition, good accuracy

Perfect
 9.6, 9.7, 9.7, 9.6, 9.5

2.2.5 Repeatability Test
 Repeatability
 CV：Run 11 tests and delete the first result, other 10 results for
analyzing.
x
i 1
i
Mean＝
n
SD 
 X i  Mean 
2
n 1
SD
CV%   100
Mean

2.2.6 Calibration
 Before calibration, please
 1) Do background test
 2) Do repeatability test
 3) Do carryover test
 At the “Count” screen, run a vial of high control three consecutive times and
then immediately run the diluent three consecutive times, calculate the
carryover per the following equation.
The calculated carryovers shall meet the following requirements: WBC, RBC
and HGB shall be no greater than 0.5 % ; PLT shall be no greater than 1%.

2.2.7 Compare
 Compare
Sample needed Volume of lyse

 Whole Blood mode 13ul 0.5ml
Prediluted mode 20ul 0.36ml
 Volumetric tube volume Diameter of aperture

WBC/HGB 500ul 100um
RBC/PLT 300ul 70um
 Ratio of dilution Whole blood Capillary blood

WBC/HGB 1:308 1:407
RBC/PLT 1:44833 1:44274

2.2.8 Parameters
Histogram Calculation Alarm
From Measurement
BC-3000 Plus can give out 20 parameters and 3 histograms
Results coming from measurement

White Blood Cell WBC Red Blood Cell RBC
Lymphocyte Lymph# Hemoglobin HGB
Mid-sized cell Mid# Hematocrit HCT
Granulocyte Gran# Mean Cell (erythrocyte) Volume MCV
Lymphocyte % Lymph% Mean Cell (erythrocyte) Hemoglobin MCH
Mid-sized cell% Mid% Mean Cell (erythrocyte) Hemoglobin
Granulocyte% Gran% Concentration MCHC
Coefficient of variation RDW-CV
Platelet PLT Red Blood Cell (erythrocyte) Distribution
Mean Platelet Volume MPV Width Standard deviation RDW-SD
Platelet Distribution
Width PDW
Plateletcrit PCT
Large Platelet ratio P-LCR

2.2.8 Parameters
Measurement From Histogram Calculation Alarm
Results derived from the histogram

Red Blood Cell Distribution
Width Standard deviation RDW-SD
Platelet PLT
Mean Platelet Volume MPV
Width PDW
Plateletcrit PCT
2.2.8 Parameters
Measurement Histogram From Calculation Alarm

Results calculated by formula
Platelet PLT Red Blood Cell Distribution
Mean Platelet Volume MPV Width Standard deviation RDW-SD
Width PDW
Plateletcrit PCT
2.2.8 Parameters
Measurement Histogram Calculation
Parameters Alarm
 Alarm
“H” or “L” is indicating the data has exceeded the setup higher or
lower alarm limit of the parameter.
If the result is “***”, it indicates that the data is unreliable or has

exceeded the higher limit of the display range.
9
If the result of WBC is less than 0.5  10 /L, the system will not
perform leukocyte differential and shows “***”.

2.2.8 Parameters
Measurement Histogram Calculation
Histogram Alarm
 Alarm
WBC differential alarm
R1 Alarm
R2 Alarm
R3 Alarm
R4 Alarm
Rm Alarm
PLT alarm
Pm Alarm
PS Alarm
PL Alarm
2.3 Liquid System
2.3.1 Components
2.3.2 Hydraulic Diagram
2.3.3 Counting Circle

2.3.1 Components-valves
2-way valve 12 pieces

(V2,V7,V8,V13,V14,V15,V16,V17,V18,V10,V11,V12)
3-way valve 6 pieces (V1,V3,V4,V5,V6,V9)
When power on, When power on,

switch open
• Three way valve Two way valve

2.3.1 Components-pump&chamber:
 1. Pressure chamber, 1piece  3. Vacuum pump (waste pump ) 1piece

 2. Pressure pump 1piece  4. Vacuum chamber 1piece
4
1 2 3
open the right door
open the left door and the cover below

2.3.1 Components-syringe&syringe motor
• Syringe:
• 2.5mL syringe
• aspirate and add lyse
• 50uL syringe
• aspirate sample (whole blood mode)
• aspirate first dilution from WBC bath
• 10mL syringe
• aspirate sample (prediluted mode)
• prime sample into the baths
• Aspirate diluent, prime diluent into the baths

2.3.1 Components-sample probe&baths
 Sample probe and wipe block
 Fluctuating motor, Rotatory
motor
 WBC and RBC count bath
Wipe block
WBC RBC
bath(inside) bath(inside)
Sample probe

2.3.2 Hydraulic Diagram

Tubing
Units
 Sensor unit
 HGB unit
 Lyse injection unit
 Diluted unit
 Volumetric unit
 Vacuum unit
 Pressure unit
 Auxiliary unit

Tubing
 Sensor unit
This part is made up of front bath, aperture, rear bath, electrodes
and seal washer. This part is the most important for the whole
instrument.
 HGB unit
This part is used to measure the concentration of hemoglobin.
 Lyse injection unit

This part includes one lyse injection motor, one syringe and one
valve.

Tubing
 Diluted unit
This part consists of 4 step motors (rotatory motor, fluctuating motor
and two syringe driving motors), 2 syringes (10ml and 50ul), wipe
block and sample probe. It is used to aspirate and dilute the sample
and clean the system.
 Volumetric unit
This part consists of glass tubes and optical couplers.

Tubing
 Vacuum unit
Vacuum is the impetus to drive the counting process of hematology
analyzer.
It is made up of vacuum chamber, valves and vacuum pump.
 Pressure unit
The pressure of this part is provided by pressure pump.
It has two functions, one is to mix-up dilution, the other is to flush-
back.
 Auxiliary unit
This part mainly refers to the connecting tube, connectors and
valves which are mainly used for connection.
2.3.3 Counting Circle

Whole blood mode: aspirate blood sample
and diluent

Clean Sample Probe , WBC bath and
RBC bath, Read HGB Blank
Read HGB Blank

Prepare WBC Sample and RBC Sample

Aspirate , add lyse, establish pressure and
bubble mixing

Cell Counting
Read HGB Sample

Cleaning bath and Cleaning pipe

Pre diluted mode: Dispense the diluent into the tube and
mix with capillary blood to make first dilution.

Pre diluted mode : aspirate blood sample and
diluent

Clean Sample Probe , WBC bath and
RBC bath, Read HGB Blank
Read HGB Blank

Prepare WBC Sample and RBC Sample

Aspirate , add lyse, establish pressure and bubble
mixing

Cell Counting
Read HGB Sample

Cleaning bath ,pipe and empty the metering
tube

Cleaning-Flush

Whole Blood Count
Counting Cycle
Read HGB blank
50ul syringe aspirate 13ul blood sample

Rinse and drain WBC bath
Dispense diluent prefill the WBC bath
 Whole Blood Mode
1:269 dilution in WBC bath
Rinse and drain RBC bath

50ul syringe aspirate 15.6ul blood dilution Dispense diluent prefill the RBC bath
Add 0.5ml lyse reagent to the WBC bath
1:308 dilution in WBC bath 1:44833 dilution in RBC bath
WBC Count
RBC/PLT Count
Read HGB sample
Rinse WBC bath Rinse RBC bath
Zap the apertures
End

Well-mixed 1:80 prediluted
Prediluted Mode Count
sample
Counting Cycle Read HGB blank

10ml syringe aspirate 0.7ml 1:80
prediluted sample
Rinse and drain WBC bath
Dispense diluent prefill the WBC bath
 Prediluted Mode
1:366 dilution in WBC bath
Rinse and drain RBC bath

50ul syringe aspirate 24.8ul blood dilution Dispense diluent prefill the RBC bath
Add 0.36ml lyse reagent to the WBC bath
1:407 dilution in WBC bath 1:44274 dilution in RBC bath
WBC Count
RBC/PLT Count
Read HGB sample
Rinse WBC bath Rinse RBC bath
Zap the apertures
End

Counting Cycle (whole blood mode)
1. The 50ul syringe aspirates 13ul of EDTA-K2 anticoagulant whole blood

into the probe. The instrument reads HGB blank. The WBC bath rinses
and drains. The 10ml syringe dispenses diluent into the WBC bath to
prefill it.
2. The probe moves to the WBC bath and the 10ml and 50ul syringes
dispense the sample (13ul) and diluent into the WBC bath, making a
1:269 dilution. The RBC bath rinses and drains. The 10ml syringe
dispenses diluent into the RBC bath to prefill it.
3. The 50ul syringe aspirates 15.6ul of the 1:269 dilution into the probe for
the RBC/PLT dilution. The 2.5ml syringe aspirates lyse.

4. The 2.5ml syringe sends 0.5ml lyse reagent to the WBC bath for a final
1:308 dilution, while the 10ml and 50ul syringes dispense 15.6ul of the
1:269 dilution and additional diluent into the RBC bath for a final RBC/PLT
dilution of 1:44833.
5. Mixing bubbles enter the baths to mix the bath contents. The vacuum
chamber drains.
6. Both dilutions (WBC and RBC/PLT) are drawn through the apertures via
regulated vacuum.

7. The instrument counts 500ul dilution for WBC and counts 300ul dilution
for RBC and PLT. After counting finishes, the flow ends.
8. The system takes an HGB sample reading.
9. The system analyzes the data while the WBC and RBC baths drains and
rinses.
10. The system zaps the apertures and the probe moves to the aspirating
position. The system displays results on the screen.
11. The system is ready for the next sample.

2.4 Hardware

Main Boards
 CPU Board
 Power Driver Board
 Analog Signal Board
 Power Supply Board
 Volumetric Board
 Keypad Board
 Recorder Board

Hardware Block Diagram
TFT-LCD

Hardware Disassembly view

CPU Board

Basic functions of CPU Board
1. Analog signals WBC/RBC/PLT counts, HGB, aperture

voltage,
vacuum/pressure signals, etc.
2. Monitor system status
+56V, +12V, -12V supplies of the analog board,
+3.3V, +12V supplies of the CPU board
temperature, liquid sensor
3. Receive the keypad signal
control keypad buzzer LCD backlight.
4. Generate control signals to control the valves, aperture
zapping,
HGB LED, current source and digital pot.
5. Drive and turn on the LCD and adjust the contrast.
6. Drive the keyboard, printer and floppy drive.

CPU Board Liquid sensor
connection LCD signal
Power supply
(12V,5v)
Keypad
Recorder
Power drive
board
Volumetric
metering board
Aspirate key
Analog signal board DOM
Temperature
Power Driver Board
 Function:
Control the rotation motor, fluctuating motor and syringe
motors;
Receives switch signal from the host to control the pump
and valve.

Power Driver Board
Power supply Rotator Fluctuating 10ml 50ul
Valves and Pumps
(12V,30V) motor motor motor motor
Position
CPU board sensor

Analog Signal Board
 Function:
Amplify the signal of WBC, RBC/PLT channels and HGB
signal;
DC-DC circuit converts 12V to 100V for zapping and 56V as
constant current source for counting;
Monitor the pressure and vacuum in chambers;
Monitor the accessorial power supplies. + 12V, 56V

CPU board
Power
supply
(+/-12V)
Vacuum/
Pressure
sensor
HGB assembly WBC counting bath RBC/PLT counting bath

Power Supply Board
 Function:
+5V,+12V sent to CPU board;
+12V and +30V sent to power driver board for valves, pumps and
motors; +12V sent to analog board for DC-DC convention.

Volumetric Board
 Function:
Control the count cycle. Each count cycle starts when the liquid
surface reaches the upper sensor and stops when the surface
passes the lower sensor.

Keypad Board
 Function:
Support the input via man-machine interface;
Buzzer and LCD backlight control circuit.
Buzzer LCD backlight adjust
Keypad board Keypad panel

Recorder Board
 Function:
Receive the data from the CPU board via serial port, process and
send them to the thermal head;
Drives the motor of the thermal head to feed paper.

2.5 System Structure

Overview

RBC Counting Bath
Note: The concave side of the aperture must face to

the front bath.

RBC Counting Bath

the front bath.

RBC Counting Bath

the front bath.

WBC Counting bath

3-way valve

3-way valve

Sample Probe Assembly

Inside Right Assembly

Syringe Assembly

Syringe Assembly

Syringe Assembly

Pump

2.6 Trouble shooting
2.6.1 Adjust Gain

2.6.2 Histogram and pulse graph
2.6.3 Trouble shooting

2.6.2 Adjust Gain

General
Adjust the Gain of the Channel in the Following

Situations:
 Replace count bath
 RBC count bath: RBC, PLT
 WBC count bath: WBC (whole blood), WBC
(prediluted) and HGB
 Replace aperture
 RBC aperture: RBC, PLT
 WBC aperture: WBC (whole blood), WBC
(prediluted)
 Replace analog signal board, CPU board, DOM
 WBC (whole blood), WBC (prediluted), RBC, HGB
and PLT

Gain of WBC Channel
 too small WBC gain that should be increased
 too large WBC gain that should be decreased

Gain of WBC Channel
 Use normal volunteers’ fresh EDTA-K2 anticoagulant

venous blood;
 Make the end position of histograms of most blood
samples between 320-350fl;
 Set up WBC (prediluted) gain the same as the WBC
(whole blood) gain.

Gain of RBC/PLT Channel
 When the difference between the actual MCV

analysis result and the expected result exceeds
6%, need to change the RBC gain.
 For example, assuming the expected MCV result
is 90.0fL, while the actual analysis result is 82.0fL,
the gain factor as below:
ExpectedMCV 90.0
＝  1.098
ActualMCV 82.0

Gain of HGB Channel
• Modify the background voltage value of HGB

within the range of 3.4-4.8V (4.5V recommended).

2.6.3 Histogram and pulse graph

Normal histogram
Fluctuating
Discriminator WBC 1 2 3 4
1 30~85fL
2, 3 85~125fL
Fixed Discriminator
4 400fL
[fL]
Lymph Mid Neutro
(Baso、Mono、Eosino)

No differential result
No differential result because the WBC histogram is over-

narrowly compressed.

No differential result because WBC count result is

less than a certain value (WBC < 0.5)

No differential result because the peak of WBC

histogram lies in the middle of the histogram and thus
cannot dentify the type of peak cells

Increased nucleated erythrocytes or interference or inadequate lyse

No lyse reagent or poor lyse

Severe interference in WBC channel

Increased lymphocytes

Increased mid-sized cells

Increased neutrophilic granulocytes

Pulse Graph
 After each count, the system can save the original sampling
pulses of this time. We can analyze the reason leading to the
fault by viewing these original data.
 Enter password “3210”, after a count, you can view the WBC
pulse graph of this count by pressing “1” and RBC pulse
graph by pressing “2” and PLT pulse graph by pressing “3”.
Press “ENTER” to exit.
 For BC2800, please press F5 and press F1 to select the

channel.

WBC pulse Graph of normal sample

WBC pulse graph of normal
background

RBC pulse graph of normal sample

RBC pulse graph of normal background

PLT pulse graph of normal sample

PLT pulse graph of normal
background

 Severe interference in WBC channel (background)

 Severe interference in WBC channel (sample)

Severe interference in RBC channel (background)

Severe interference in RBC channel (sample)

Severe interference in PLT channel (background)

Severe interference in PLT channel (sample)

Interference occurs because gain of PLT channel is too large

Slight interference in WBC channel

Inadequate or no hemolysis in WBC channel

Slight interference in RBC channel

Slight interference in PLT channel

Interference in WBC channel caused by inverter Feature: sine wave

Interference in WBC bath from inverter

Insufficient liquid in WBC bath during count

Interference in RBC channel from tubing

Insufficient liquid in RBC bath during count

Interference in PLT channel from tubing

2.6.3 Troubleshooting

Background abnormal
Background  Not well grounded

abnormal
 Shielding cover of bath and analog board
WBC(RBC) clog
 Electrical interference
WBC(RBC) bubble
 Reagents dirty
Vacuum low  Bath, valve or tube dirty
Pressure low  Wipe block, diluent dispenser, shielding cover,
Results abnormal
 10mL syringe dirty
 Plastic washer dirty, aging
WBC abnormal
 Replace bath
10mL syringe error
 Replace analog board
Display problem  Pulse graph
No start up

WBC(RBC) Clog
Background
abnormal
 Reagents empty
WBC(RBC) clog
 Diluent added into the bath sufficiently
 Zap, flush
WBC(RBC) bubble
 Aperture voltage: 8~12V(WBC), 12~16V(RBC), if high,
Vacuum low
unclog
Pressure low  Watch volumetric board
Results abnormal  Fluid pass normal, volumetric board defective or need
adjustment, CPU board
WBC abnormal
 Fluid pass abnormal, check valve 8(18), tube between
10mL syringe error
valve 8(18) and bath
Display problem
No start up

WBC(RBC) Bubble
Background
abnormal
 Reagents empty
WBC(RBC) clog
 Diluent prime, rinse prime
WBC(RBC) bubble  Diluent added into the bath sufficiently
Vacuum low  Watch volumetric board
Pressure low
 Fluid pass normal, volumetric board defective or need
adjustment, CPU board
Results abnormal
 Fluid pass abnormal, check valve 8(18), tube between
WBC abnormal
valve 8(18) and bath leak or not
10mL syringe error
Display problem
No start up

Vacuum low
Background
abnormal  System test->Vacuum
WBC(RBC) clog  Vacuum filter
 Waste container full
WBC(RBC) bubble
 Waste tube bent
Vacuum low
 Tube connections between valve(7,17,8,18,10,2,11,12)
Pressure low  Valve(7,17,8,18,10,2,11,12)
Results abnormal  Waste pump block, clean
WBC abnormal
 Vacuum chamber leak
 Tube between vacuum chamber and analog board
10mL syringe error
 Analog board
Display problem
No start up

Pressure low
Background
abnormal  System test->Pressure
WBC(RBC) clog  Pressure filter
 Tube connections between pressure chamber,
WBC(RBC) bubble
pressure pump, valves, analog board
Vacuum low
 Valve 9,15,16
Pressure low  Pressure pump
Results abnormal  Pressure chamber
WBC abnormal
 Analog board
10mL syringe error
Display problem
No start up

Results
abnormal
Background
abnormal  Check blood sample
WBC(RBC) clog  Check reagents
 Check background
WBC(RBC) bubble
 Do QC test
Vacuum low
 Use control to check CV
Pressure low  Calibration
Results abnormal
WBC abnormal
10mL syringe error
Display problem
No start up

WBC
abnormal
Background
abnormal  Check lyse
WBC(RBC) clog
 Lyse test
 Lyse not enough, check valve 6 and relevant tube
WBC(RBC) bubble
 Lyse enough, then
Vacuum low
 Check background
Pressure low  Check repeatability of WBC, good then calibrate
Results abnormal  Repeatability of WBC not good, check relevant parts
WBC bath, valves12, tubes, analog board
WBC abnormal
10mL syringe error
Display problem
No start up

10mL syringe error
Background
abnormal  System Test-> 10mL syringe motor
WBC(RBC) clog  Remove tubing connected to syringe, then test again
 Add lubricating oil to axis of motor
WBC(RBC) bubble
 Syringe sensor dirty or loose
Vacuum low
 Disassemble and clean 10mL syringe
Pressure low  Check optical sensor cable, motor cable to power driver
Results abnormal board
WBC abnormal
 Optical sensor defective
 Motor defective
10mL syringe error
 Power driver board defective (other motor might have
Display problem
error too)
No start up

Display problem
Background
abnormal  Check and adjust backlight, contrast
WBC(RBC) clog  Check cable between LCD screen and CPU board
 No backlight? Converter board defective?
WBC(RBC) bubble
 Replace the cable
Vacuum low
 Replace LCD screen
Pressure low  Replace CPU board
Results abnormal
WBC abnormal
10mL syringe error
Display problem
No start up

No startup
Background
abnormal  Power, fuse
WBC(RBC) clog  Disconnect all boards from power supply, use
multimeter to measure output
WBC(RBC) bubble
 Only connect CPU board, analog board, if no start,
Vacuum low
power supply board, CPU board, DOM
Pressure low  Remove motors, valves, pump from power driver board
Results abnormal  Connect CPU board, power driver board, if no start,
WBC abnormal
power driver board defective
 Short circuits in valves, pumps, motors
10mL syringe error
Display problem
No start up

2.7 Maintenance

Daily maintenance
If it works day and night or the sample volume is more than 100
per day, execute “E-Z cleanser cleaning” operation once every
day.
If the quantity of samples more than 50pcs, set the “Auto clean
time” 4 hours.
If the user turns off the instrument every day, the user should
execute “probe cleanser cleaning” operation once every week.
Or do it when the machine remind you.
Use E-Z cleanser to execute shutdown program.
Dispose the waste liquid

Monthly maintenance
 Use probe cleanser to clean the sample probe wipe block.
 Use sample probe localizer to correct the position of the sample probe.
 Calibrate the unit.

Correct the sample probe
Procedure
1. Take off the right and front door of the equipment.
2. Input password, then access the “System Test” menu in “service”
3. Select the “Fluctuating motor” press [↑][↓] to drive the motor
4. Move up the sample probe to the end
5. Loose the screw fixed the probe
6. Using localizer to correct the probe and then tighten the screw
Localizer

Half-year maintenance
 Replace vacuum filters, if necessary, should
replace the pressure filters.
 Test all valves’ work status.
 Clean the valves, especially the V11 and V12.
 Disassemble and clean the two baths and
apertures.
 Check the stability of HGB blank voltage.
 Check the stability of vacuum.
 Clean inside of the equipment.

Version Information
Version Author Date Description
1.0 Tata Ren 8/1/2012 N/A
2.0 Rany 6/12/2014 N/A

Check Information
Version Reviewer Date Comments

1.0 Bruce 8/3/2012 N/A
2.0 Mars Zhang 7/10/2014 Approved

Thank you!

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BC-3000 plus Service Training

Hematology Global Technical Support Department

2 © 2011 Mindray Confidential

3 © 2011 Mindray Confidential

Blood Cell Leukocyte Erythrocyte Platelet

 Blood sample consists of two parts.

4 © 2011 Mindray Confidential

 There are three kinds of blood cells.

Leukocyte Erythrocyte Platelet

6 © 2011 Mindray Confidential

Blood Blood Cell Erythrocyte Platelet

Lymph Baso Mono Eosino

Lymphocyte Mid-sized Cell Neutrophilic Granulocyte

Blood Blood Cell Leukocyte Platelet

 The function of RBC is almost completed by hemoglobin (HGB).

Transport oxygen and carbon dioxide

8 © 2011 Mindray Confidential

Blood Blood Cell Leukocyte Erythrocyte

9 © 2011 Mindray Confidential

10 © 2011 Mindray Confidential

11 © 2011 Mindray Confidential

12 © 2011 Mindray Confidential

1、blood cell of Same size, go through the

2、blood cell of Same size, go through the

3、blood cell of different size, go through

14 © 2011 Mindray Confidential

15 © 2011 Mindray Confidential

 From Pulse Graph to Histogram

16 © 2011 Mindray Confidential

From Pulse Graph to Histogram

17 © 2011 Mindray Confidential

Lower sensor Lower sensor

1 Empty when start 2 Liquid surface fall down through the

Upper sensor Upper sensor

Lower sensor Lower sensor

3 Counting start when the 4 Counting end when the liquid

18 © 2011 Mindray Confidential

 HGB is determined by the colorimetric method.

19 © 2011 Mindray Confidential

Light Absorbing Detector Signal

LED light Bath Photosensor

20 © 2011 Mindray Confidential

 Reagents are one part of the system.

21 © 2011 Mindray Confidential

22 © 2011 Mindray Confidential

Installation Requirements 2.1.1 Installation Requirements

23 © 2011 Mindray Confidential

 Relative humidity: 30% to 85%.

 Atmospheric pressure: 70 kPa to 106 kPa.

 free as possible from dust, mechanical vibrations, loud noises,

 Do not place near brush-type motors, flickering fluorescent lights,

 Do not place in direct sunlight or in front of a source of heat or

24 © 2011 Mindray Confidential

 At least 40 cm on each side, which is the preferred access to

 At least 10 cm behind for cabling and ventilation

 There is enough room on or below the countertop to accommodate

25 © 2011 Mindray Confidential

 Check the availability of a power outlet that is

26 © 2011 Mindray Confidential

1 ---- Display Screen

4 ---- Power Indicator

5 ---- Aspirate Key

6 ---- Sample Probe