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• MERISTEM CULTURE
= in vitro culture technique which uses
meristem as an explant.
Longitudinal section of a shoot tip
Meristem A clump of rapidly dividing
cells
Primary Secondary
meristem meristem
Leaf meristem
Root
meristem
HISTORY
White (1934)
Tobacco Mozaic Virus (TMV) was unevenly distributed at
different zone of tobacco root. It’s concentration was lower
near the root tip, and the root tip itself was free from virus..
Limasset dan Cornuet (1949)
There was a virus gradient at tobacco root, where it’s mersitem
was virus free.
Morel dan Martin (1952)
Isolated an apical meristem of virus-invected Dahlia to
produce virus free plants.
GENERAL
PRINCIPLE
Meristem culture technique is carried out
with a view to obtain a virus free plant from
virus-invected plants.
Meristem culture is used to eliminate virus, so
virus will not be distributed which leads to
the production of virus free plants.
Mechanism
Meristem culture
Heat treatment (35-38 oC)
Effective against viruses and mycoplasma
Can be ineffective to plant which is sensitive to heat
tanaman yang
propagasi lebih lanjut
terinfeksi virus
dari tanaman bebas
penyakit
(perlakuan panas)
tanaman bebas
kultur meristem virus
(uji virus)
multiplikasi tunas
memindahkan
pembentukan akar tanaman ke tanah
dari tunas