Professional Documents
Culture Documents
Platelets
Numbers
CBC
PLT count
PLT morphology
Function
Bleeding Time (BT)
Platelet Aggregation
Whole blood aggregation
Platelet-rich plasma aggregation
2 Islamic Unversity of Gaza 10/11/2010
Platelet Structure
• PRIMARY HEMOSTASIS
• Form platelet plug:
damaged endothelia
• Nurture endothelia
• SECONDARY HEMOSTASIS
• Reaction surface for
coagulation
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8 Islamic Unversity of Gaza 10/11/2010
Platelet Plug Formation: Aggregation
Platelet-Platelet interaction
Mechanism components
ATP
Ionized calcium
Fibrinogen
PLT receptor GPIIb/IIIa
Initial aggregation – REVERSIBLE
Secondary aggregation – IRREVERSIBLE* =
white clot, a.k.a platelet plug formed.
Platelet Plug Formation: Secretion
Discharge of
granules’ contents
Markers of PLT
activation*
PF4
PDGF
Thromboglobulin (β-TG)
Promote & Amplify PLT
activities
Primary hemostasis
Secondary hemostasis
Inherited Platelet Disorders- Qualitative
Qualitative disorders
Adhesion
Bernard-Soulier syndrome ( GP Ib-IX )
Platelet-type (Pseudo-) von Willebrand disease
( GP Ib receptor) *
Collagen receptor deficiency (GP VI)
Aggregation
Glanzmann thrombasthenia (Gp IIb-IIIa)
Secretion
Dense (δ) granule defects (storage pool
deficiency)
α granule defects (gray platelet syndrome)
Platelet procoagulant activity
Scott syndrome PF3
Islamic Unversity of Gaza 10/11/2010
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Platelet Activation (signaling)
Collagen*
ADP*
Epinephrine*
Arachidonic acid*
The antibiotic ristocetin*
Thrombin
Serotonin
Snake venoms, antigen-antibody complexes, soluble
fibrin monomer complexes, and fibrin(ogen) degradation
products (FDPs).
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PRP Aggregometry Agonist & Patterns
Thrombin
Biphasic curve. Irreversible aggregation only (does not
require cyclooxygenase)
Arachidonic acid
2o wave only; assesses cyclooxygenase pathway
Serotonin
o A primary wave of aggregation with a maximum of 10% to
30% transmittance followed by disaggregation.
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Bernard-Soulier syndrome
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Platelet storage granule defects
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Comment
(Pronounce: ab-SIKS-ih-mab)•
ReoPro
inhibits GP IIb/IIIa