Professional Documents
Culture Documents
Selecting a clone Screening a clone bank Translation of mRNA in vitro Restriction mapping
Labelled
probe
which may be
• Using specific probes that are labelled specific sequences of DNA can be
identified.
• There are three main hybridization techniques which vary in the sample
blotted and the probes used;
1. Northern Blot-Transfer of an RNA sample separated and identified using
DNA or RNA probes.
2. Southern Blot-Transfer of an DNA sample separated and identified using
DNA or RNA probes.
3. Western Blot- Transfer of an Protein sample separated and identified
typically using an antibody.
Applications