Equipment & Materials

Equipment : Materials :
• Stirring Rod. •Measuring cup
• Aquadest
• Blender • Gauze
• Diethyl Ether
• Funnel • Filter paper
• Ethyl Acetate
• Separate funnel • Whatman
• HCl 2 N
• Desiccator • Round bottom flask
• Methanol
• Erlenmeyer • Analytical balance
• N-butanol
• Beaker glass • Oven
• Petroleum ether.
• Test tube • Pipette
• Waterbath. • Rotavapor
Sample preparation
Wash the Lidah Mertua leaves
with running water which have
been cut into pieces, and dried Mashed the leaves using a
it by aerating without direct blender.
sunlight for 4 hours and oven
at 50 ° C .

Saponin Identification

Cool it down for a moment

Insert 0.5 g of simplicia powder
before shake it vigorously for
of Old Aloe leaf in a test tube,
10 seconds until a solid foam is
then added 10 ml of hot water.
formed.

And then added 1 drop of HCl 2 N

through the test tube wall. On
additions of 1 drop of HCl 2N, if the
foam does not disappear, It means
the sample contains saponins.
Extraction process.
Separated The methanolic
50 grams of Lidah Mertua essence from the pulp.
leaves powder is refluxed with Evaporated the juice which has
methanol (1:10) for 30 minutes. been separated with the rotary
evaporator until coagulate.

Weighed the condensed extract

which is obtained from rotary
evaporator and calculated the
yield.

Determination of saponin.
After cooling, the petroleum
ether solution is removed and the
1.25 grams of extract is refluxed
residual residue is dissolved in 50
with 50 ml of petroleum ether
ml of ethyl acetate. The solution
at temperature 60-80 ° C for 30
is transferred to a separating
minutes.
funnel then separated from the
ethyl acetate solution.
Discarded the Petroleum ether
solution and dissolved the
remaining residue in 50 ml of
ethyl acetate. The solution
which is transferred to a
separating funnel is separated
from the ethyl acetate solution
s o o n a f t e r .
Dissolved The remaining The remaining
residue with 50 ml of n- evaporation is dissolved
butanol 3 times, with 10 ml of methanol
re s p e c t i ve l y. A l l then the solution is
butanolic solutions were dripped into 50 ml of
mixed and evaporated at diethyl ether while
the rotary evaporator. stirring.

When the filter paper had Poured the sediment which is

dried, weighed until the weight
fixed. The difference in weight
of filter paper before and after
filtering is determined as the
saponin weight.
formed in the mixture on filter
paper which weight is known,
then rinsed the sediment on
paper with 10 ml of diethyl
ether.