Evaluation of antioxidant and antimicrobial activity of Ipomoea mauritiana plant

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and crude fish bile of Pangasius pangasius and Tenualosa ilisha.
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Istiak Alam, AKM Moyeenul Huq*
Department of Pharmacy, School of Science and Engineering
Southeast University, Banani, Dhaka-1213

Abstract Methods and Materials Results (ctd..)

The antioxidant and antimicrobial activities of Ipomoea Preparation of the extract Antimicrobial test
mauritiana and the antimicrobial activity of Pangasius pangasius Ipomoea mauritiana plant were collected from Sokhipur, Mymensing district of Bangladesh. Powdered plant  Ipomoea mauritiana
and Tenualosa ilisha fish bile were assessed. Ipomoea mauritiana materials are soaked in methanol to get methanolic extract of I. mauritiana.
whole plant was extracted in methanol and the total phenolic and Collection of bile samples
flavnoid content was determined. The antioxidant activity was The Pangasius pangasius (1–1.5 kg) and Tenualosa ilisha (400–800 gm) were purchased from local market. The bile
determined by DPPH free radical scavenging and ferric reducing was collected from intact gall bladder. The collected bile was then preserved at 0ºC for further study.
power activity methods. The antimicrobial activity was Collection of Microbes
evaluated by disk diffusion method against gram positive and Tested 4 gram positive and 6 gram negative bacteria's were collected from the Microbiology Department Lab of
Dhaka University.  Pangasius pangasius
gram negative bacteria using kenamycin (30 µg/mL) as standard.
 Antioxidant Study.
The total pheolic and flavonoid contents of the extract were
 The total phenolic content was determined by the Folin–Ciocalteu method.
59.30 and 27.21 mg of GAE/gm and QE/gm respectively, while  The total flavonoid content was determined by the aluminium chloride colorimetric method.
the IC50 of ferric reducing power activity was 275.08 µg/mL for  Free radical scavenging activity was experimented by DPPH method.
the methanol extract as compared to ascorbic acid (IC50,  The ferric reducing capacity was investigated by using the potassium ferricyanide-ferric chloride method [2]
230.09µg/mL). I. mauritiana methanolic extract was active  Antimicrobial Study  Tenualosa ilisha
against all test organisms with the highest zone of inhibition  Antimicrobial study was done by Disk Diffusion method [2]
against Bacillius cereus (19.25mm). The crude bile of P. Results
pangasius and T. ilisha showed antibacterial activity against all Anti-oxidant test
the bacteria of the both strains where the bile of the earlier
species was most active against Shigella boydii (22.3 mm) and Antioxidant test Concentration GAE GAE c*V mg C=(c*V) Mean
Salmonella paratyphi (22.8mm). T. ilisha bile was most active (sample) c c /m mg/gm ± STDEV
against Vibrio parahemolyticus and Shigella dysenteriae (19.4 µg/ml µg/ml mg/ml
and 18.5 mm respectively). The results indicate that I. mauritiana Total phenolic 200 12.32±1.25 0.012±0.01 0.012±0.015 55.627±2.52 58.302±3.28 Here,
possesses antioxidant and antimicrobial property and P. content A= Standard (Kanamycin), B=Blank (DMSO)
pangasius, T. ilisha bile have antimicrobial activity. C= Tested Sample , D= Sample+DMSO
Total flavonoid 200 5.514±0.95 0.005±0.07 0.005±0.078 27.57±1.38 28.212±1.50
activity
Background
Here, GAE=Gallic acid equivalent, C= Total content of phenolic compounds, mg/gm plant extract in GAE, c = The concentration
It is evident from the literature that various species of Ipomoea of gallic acid established from the calibration curve (mg/gm),V=volume of the crude plant extract in gm.
genus are used to treat various mild to fatal diseases and possess
DPPH free radical scavenging test Ferric Reducing Capacity Test
antioxidant properties. Moreover there is lack of enough scientific
report to confirm the antioxidant properties and antimicrobial
activity of Ipomoea mauritiana plant. On the other hand Microbial
resistance has become an increasing global problem and there is a
need to find out novel potent antimi-crobial agents with alternative
modes of action as accessories to antibiotic therapy. Moreover,
scientific studies have found that bile acid and salts have
antimicrobial activity against intestinal microbes. [1] Therefore,
the current study was designed to evaluate the antioxidant
properties and antimicrobial activity of methanolic extract of
Diameter of zone of inhibition (mm)
Discussion
Ipomoea mauritiana plant and crude bile of with Pangasius
Test Kanamycin I. mauritiana P. pangasius T. ilisha
pangasius and Tenualosa ilisha. microorganism (30 μg /disc) (400 μg /disc) (30 μg /disc) (30 μg /disc) As Ipomoea mauritiana plant rich in phenolic compound so this
Gram positive bacteria
44.22 ± 00 19.25±1.020
plant is a potent source of antioxidant. Previously reported that
Tasted Samples Bacillus cereus 20.8 ± 0.020 18.2 ± 0.406
bile possess antimicrobial
Bacillus subtilis 38.25 ± 0.367 14.25±0.612 21.25 ± 0.612 16.5 ± 0.514
Staphylococcus 43.67 ± 0.752 0 17.5 ± 0.0816 14.4± 0.271
aureus Conclusions
Sarcina lutea 39.45 ±0.981 18.5±0.0813 18.2±0.112 19.80±0.852
Gram negative bacteria
Escherichia coli 42.66 ± 0.714 0 19.15 ± 0.122 16.51 ± 0.122
The result shows that Ipomoea mauritiana possess antioxidant
Pseudomonas 40.45 ± 0.468 13±0.816 18.3 ± 0.816 17.36 ± 0.560 and antimicrobial property. Crude fish bile of Pangasius
aureus pangasius and Tenualosa ilisha exert potent antimicrobial
a b c Salmonella 39.34 ± 0.618 15.15±0.122 22.8 ± 0.857 15.02 ± 0.318
activity.
a. Ipomoea mauritiana b Pangasius pangasius. c, Tenualosa ilisha paratyphi
Shigella 42.53 ± 0.388 0 17.01 ± 0.122 18.57 ± 0.262
dysenteriae
Shigella boydii 41.64 ± 0.255 18.25 ± 3.06 22.25 ± 3.06 17.25 ± 0.06 Acknowledgements
Contact Information Vibrio 40.03 ± 0.362 References16 ± 0.816 18.18 ± 0.816 19.45 ± 0.348
parahemolyticus We like to express special thanks of gratitude to Department of Pharmacy,
Istiak Alam 1. Begley, M., Gahan, C. G. M., & Hill, C. (2005). The interaction between bacteria and bile.
FEMS Microbiology Reviews, 29(4), 625–651. Southeast University for providing us required facilities and support to
Email: istiaksoikot@gmail.com
2. Israt Jahan Bulbul and Nisrat Jahan (2016), Study on antioxidant and antimicrobial complete this research within time frame
Corresponding author: Akm. Moyeenul Huq
E-mail: huq_pharmacy@hotmail.com activities of methanolic leaf extract of Glycosmis pentaphylla against various bacterial
strains, Journal of Pharmacognosy and Phytochemistry, 5(4): 53-57