APPLICATION OF ENZYME IN AGRICULTURE.

THE QUANTITATIVE METHODS TO DETERMINE CELLULASE

ACTIVITY
GROUP MEMBER :

Nguyễn Thị Hạnh Duyên BTBTIU15101

Diệp Thị Thanh Tâm
Nguyễn Lê Trà My BTBTIU15087
Nguyễn Thị Thanh Lam BTARIU16025
OUTLINE

I.Application of enzyme in agriculture.

 In animal feed
 In plant growth
II.The quantitative methods to determine cellulase activity.
I.APPLICATION OF ENZYME IN AGRICULTURE.

 In animal feed

Spray enzyme To keep insects

surrounding away
animal farm

Adding enzyme Animal wastes

Enzyme added become
in the feed (dry
into water odourless and
stuff or grass)
natural fertilizer
I.APPLICATION OF ENZYME IN AGRICULTURE.

 In animal feed
I.APPLICATION OF ENZYME IN AGRICULTURE.

 In animal feed
 The pond has the animal wastes. The enzyme can resolve
→ The plant can grow on the pond.

 The fish on the pond eat wastes animal to can grow bigger
→ Enzyme take from wastes animal help the fish absorb nutrient.
I.APPLICATION OF ENZYME IN AGRICULTURE.

 In plant growth
Enzyme is the natural nutrientfor the soil.
Vegatable → can grow healthily
within the short period.
The transformation of sandy to
rich healthy farmland.
I.APPLICATION OF ENZYME IN AGRICULTURE.

 In plant growth
 The enzyme helps to boots the process of blooming.
 The root is also the stronger.
The Orchis plants are sprayed with enzyme
I.APPLICATION OF ENZYME IN AGRICULTURE.

 Soil enzymes play an important role in organic matter

decomposition and nutrient cycling
 Some enzymes only facilitate the breakdown of organic matter
(e.g., hydrolase, glucosidase), while others are involved in
nutrient mineralization (e.g., amidase, urease, phosphatase,
sulfates).
II.THE QUANTITATIVE METHODS TO DETERMINE CELLULASE
ACTIVITY.

 Cellulase activity reported a lot of determination methods:

- Filter paper collapsing method
- Thread cutting method
- Flat band method
- Branch and swain method
- CMC method
II.THE QUANTITATIVE METHODS TO DETERMINE CELLULASE
ACTIVITY.

 Filter paper collapsing method

-The constant temperature oscillator use certain rules of the filter paper as
substrate and adding a tube containing enzyme solution, micro-oscillation
were carried on an optimal temperature and pH.
-After the determined time required for the complete collapse of the filter
paper, we can obtain the enzyme activity.
-To determinine the filter paper collapse time is difficult, so the error is
large.
 II.THE QUANTITATIVE METHODS TO DETERMINE CELLULASE
ACTIVITY.

Step 1 : Measure time required

Step 2 : Measure of amount
substrate
Step 3 : Measure of enzyme
concentration
II.THE QUANTITATIVE METHODS TO DETERMINE CELLULASE
ACTIVITY.

Enzyme units
One unit (U) enzyme activity: the amount of enzyme required to
liberate 1 µmol reducing sugar from the appropriate substrate per
min under the assay conditions.
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