Writing Problem and Hypothesis

Statements for Science Research(13)

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Setting of work proposal :
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Work problem :
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Quantitative specification of problem :

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Importance of problem :
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Project need : ,
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Work objective : ?
Methodology to achieve objective
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Anticipated results :
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Contribution to field :
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()
Commonly found in legume seeds, proteinase inhibitors play a
critical role in regulating several physiological processes such as blood
coagulation, complement fixation, fibrinolysis, and fertilization. As proteins
having characteristic polypeptide scaffolds, most of these inhibitors are
grouped into several families including the Kunitz, Kazal, Burman Birk, Serpin
and mucus families. As well established, the production, invasion and transfer
of cancer cells are significantly related to proteinase inhibitors.
However, previous efforts have failed to suppress cancer proteinase inhibitors
effectively. (NOTE : Add 3-4 sentences that describe characteristics of the
problem or statistics that reflect its severity) owing to the
inability to identify proteinase inhibitors that can suppress more than 80of all
cancer cells. Consequently, the inability to understand
the physiological role of proteinase inhibitors in plants makes it impossible to
ultimately understand the role that proteinase inhibitors play in cancer cells.
Therefore, the coffee senna trypsin inhibitor (CSTI), to which
Coffee Senna, i.e. a leguminosae that belongs to its bean sub-family, must be
purified and characterized by using protein purification. Essential to
understanding the fundamental principles of protein purification, protease
inhibitors must be used to design new substances, capable of effectively
controlling diseases and pathological processes.
()
The coffee senna trypsin inhibitor (CSTI), to
which Coffee Senna, i.e. a leguminosae that belongs to its bean
sub-family, can be purified and characterized by using protein
purification. Essential to understanding the fundamental
principles of protein purification, protease inhibitors can be used
to design new substances, capable of effectively controlling
diseases and pathological processes. To do so,
coffee senna seeds can be analyzed. CSTI can then be
extracted using a crude extraction method containing ammonium
sulfate precipitate. Next, the senna trypsin inhibitor can then be
purified to utilize the column (i.e., gel filtration, affinity
chromatography, and ion exchange column) of various
characteristics (i.e., molecular size, molecular charge, as well as
molecular polar and affinity). Additionally, the purified protein can
be analyzed to further characterize its heat stability and different
SDS concentrations in order to achieve stability.
()
As anticipated, analysis results can indicate
that CSTI can be extracted and the type of coffee senna
seed can be identified as well. Besides characterizing CSTI
precisely through analysis, its extraction and
characterization can be used to classify the coffee senna
type. Moreover, the CSTI sequence can achieve sequential
alignment of the protein with other legumes. In
addition to providing insight into the physiological
significance of CSTI in plants, results of this study can
contribute to efforts to design substances capable of
effectively controlling diseases and pathological processes.
(NOTE : Add 2-4 more sentences that describe more
thoroughly how the proposed method contributes to a
particular field or sector)
()
Consumers demand shampoos with natural ingredients
and without any harmful side effects. Manufacturers normally select
natural plant additives and attempt to reduce the chemical composition
of such additives. This combination often implies a relatively high retail
price of shampoos. Although traditional herbs from
South Africa are frequently used in shampoo, the use of China herbs in
shampoo has received little attention. Chinese herbs often depend on
the use of fine trauma medicines. Although non-toxic and mild, Chinese
herbs are seldom used in commodities such as shampoo. Certainly
commercial applications of Chinese herbal medicine still have many
limitations, e.g., whether one ingredient has a particular effect, whether
valid ingredients can be extracted purely, or whether the usefulness of
the ingredient is depleted during the purification process, these
problems. These issues require further study. While Chinese herbal
medicine has been extensively studied for its oxidation ability to repress
cancer cells, its potential applications for external treatment such as in
shampoos has seldom been addressed.
()
In addition to numerous investigations,
Chinese herbal medicine can be found in many commercial
products. However, with the growing market in pharmaceutical
drugs and the health foods sector, Chinese herbal medicine has
many attractive features, e.g., anti- oxidation capability,
suppression of fungi, anti-cancer capabilities, toxicity analysis,
and analysis of particular ingredients for research purposes.
Despite the extensive study of herbal
medicine for therapeutic treatment purposes that has yielded
excellent results with respect to antimicrobial activity of herbal
plants, Chinese herbal medicine has seldom been studied with
respect to its toxicity levels and potential industrial applications.
Therefore, a novel formulation involving the use of
Chinese herbal medicine must be developed to replace that of
the widely used South African herbal medicine.
()
A novel formulation involving the use of Chinese herbal
medicine can be developed to replace that of the widely used South African
herbal medicine. To do so, Chinese herbal medicine can be
analyzed to suppress gram (-) bacteria. Cell culturing method is then adopted
to understand why Chinese herbal medicine does not have negative side
effects. Next, GC and HPLC methods can be used to understand the
adaptability of incorporating compounds of Chinese medicine in shampoo. The
proposed formulation can enable five varieties of Chinese herbal medicine to
achieve a better outcome than South African herbal medicine can in terms of
suppressing gram (-) bacteria. As anticipated, analysis results can
indicate that , while traditional South African herbs can restrain the
microorganism effect with a success rate of 90 upwards, Chinese herbs have
the same success rate of 99 upwards. This quality makes Chinese herbs
highly promising for efforts to restrain microorganisms and achieve safe testing,
implying safe production and substantial product revenues. The ability to
suppress germs would enable use of flow cytometer examination PGE2 to
understand its anti-oxidation activity, thus making Chinese herb medicine highly
promising for several commercial applications, including treatment for athletes
foot and the skin of head tinea owing to its ability to effectively suppress fungi,
as well as treatment of external injuries owing to its ability as a disinfectant of
germs.
()
In addition to providing a valuable reference
for Chinese herbal medicine research, the proposed
formulation can provide local healthcare product
manufacturers with a viable alternative to developing
shampoos. Its desirable features of effectively suppressing
both fungi and fungi owing to its anti-oxidation capability
further increase its commercial potential. (NOTE : Add 2
sentences that describe more thoroughly how the proposed
method contributes to a particular field or sector)
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