Ph. D.

Proposal
Epidemiological study and molecular characterization of
Hydatid Cysts Isolated from human and some
Intermediate Hosts in Sulaimanya Province.

By
Abdullah Ahmed Hama
M.Sc. Biology/ Parasitology 2007

Supervisor
Prof. Wijdan Mero
 Introduction
Hydatid disease is a silent zoonotic infection of
man and domestic animals caused by the larval
.stage of the small dog tapeworm
Man becomes infected accidentally by ingesting
the eggs of the parasite through hands, food, drink
.or other materials contaminated with dog feces

The commonest sites of infection are the liver

and lungs. The disease can not be transmitted
between humans or from human to dogs

Introduction
Hydatid cyst after surgery
The disease is endemic or hyper endemic in
most of the Middle East countries mainly in
.communities where animal husbandry is common

Ten intra-specific variants, or genotypes (G1-G10),

.have been defined based on genetic diversity

Introduction
The identification of the strains responsible
for human echinococcosis is nowadays a
significant point which has to be taken into
consideration in order to focus and to adapt
the control measures and the means of
.diagnosis
: The life cycles
Life cycle involve two mammalian hosts .The adult cestode
inhabits the small intestine of a carnivore and produces eggs
containing infective oncospheres. Either cestode segments
.(proglottids) containing eggs or free

eggs are released from the intestinal tract of the carnivore in to

the environment. After oral uptake of eggs by an intermediate
host animal, a larval stage, the metacestode, develops in
internal organs. Typically, the mature metacestode produces
.numerous protoscoleces
Life cycle
Echinococcus species life cycle

Life cycle
Location of the cyst
 Aim of this study
: The objectives of the present study are to

1. Investigate the status and prevalence of hydatid disease inhuman

and animal in Sulamania province .

2. Determine the risk group of echinococcus by immunological (ELISA)

screening.

3. An attempt to determining dynamic of echinococcus egg

transmission depending on occupation.

4. Determine the common strain which infect human in sulaimania.

5. Molecular characteristic and genetic diversity (genotyping) of

E. granulosus in different district sulaimania.

Aim
 Sample Collection

1
from animal Epidemiological analysis for 5
year for human and animals

Histological confirmation
Direct M.E sulaimania
kalar
Protoscolecies by centerfugation Germinal layer
halabja
penjween
DNA extraction Saeed sadq

PCR amplification of CO1 gene and using Rania

specific primer

Genetic diversity (genotyping) for human, goats, sheep and cattle

Methods and sampling

 2 Human

cyst from liver lung, spleen and other organ by 10 ml Serum from
surgery Butcher
Shepherd
Germinal layer Aspiration of fluid
Grocer
separated
House wife
Hisological conformation D. M. Examination
animal husbandry
Abattoir worker
DNA extraction
Veterinarian
Control normal person
PCR amplification of CO1 gene and
using specific primer
ELISA IgG detection
Genetic diversity (genotyping) for human, goats,
sheep and cattle
Positive Negative
End