Professional Documents
Culture Documents
Organisms are not immortal. They die due to aging, predation, diseases, natural disasters & accidental deaths. Therefore the ability to reproduce in order to ensure continuity of the species, is one of the fundamental characteristics of all living organisms.
Reproduction 2 modes
Asexual reproduction a single individual is the sole parent and passes copies of all its genes to its off spring Sexual reproduction two parents give rise to offspring that have unique combinations of genes inherited from each parent
Asexual Reproduction
Rapidly reproduces large numbers of individuals, usually have an identical genetic composition to each other and to the single parent from which they are derived.
production of an individual from a parent without the fusion of gametes new organism is exactly like its parent takes place under favourable conditions and it results in a rapid growth of the population
Asexual Reproduction 1 Fusion of gametes 2 Type of cell division 3 Genetic variety none mitosis usually identical
Asexual Reproduction 1 Fusion of gametes 2 Type of cell division 3 Genetic variety 4 Adaptation to environment none mitosis usually identical less adapted
Asexual Reproduction 1 Fusion of gametes 2 Type of cell division 3 Genetic variety 4 Adaptation to environment 5 Number of parents none mitosis usually identical less adapted one
Sexual Reproduction yes meiosis great genetic variety better adapted two
Asexual Reproduction
There are several types of asexual reproduction: (1) binary or multiple fission, (2) Budding, (3) Fragmentation, (4) Sporulation, (5) Vegetative propagation
Binary or Multiple Fission - This occurs in single-celled organisms, e.g. protozoa and bacteria
Parameucium
In bacteria: Under favourable conditions, bacterial DNA replicates first, then nucleoplasm divides into two, followed by the cell as a whole.
Under unfavourable conditions, the endospore forms which is resistant to desiccation, extremes of temperature and toxic chemicals.
Budding - by yeast (a unicellular fungus) reproduces asexually under favourable conditions - differs from binary fission in that the new part produced is smaller in size than the parent
Budding in Yeast
other examples: - flatworms, annelids, cnidarians - In colonial cnidarian Obelia: blastostyles give out buds called medusae for sexual reproduction
Budding in hydra
Fragmentation - In Spirogyra, portions of the filamentous alga break away when the filament reaches a certain length. This drift away, attach themselves elsewhere and begin vegetative growth again. - Regeneration: cell division to regenerate the missing parts of a body as a result of injury. - Fragmentation: an organism regularly and spontaneously divides itself up.
Sporulation
Mucor, a saprophytic mould living on decaying bread
- hyphae: spreading the colony into a mycelium - sporangium: contains spores - sporangiophore: erect hyphae with columella at tip - spores: liberated when sporangium matures; small & light weight; can be carried over great distances by wind; germinate into new mycelium when suitable substratum is found
Disadvantages
Overcrowding may occurs as the daughter plants are close to the parents to cause competition for space, light as well as water. The next generation cannot be improved the quality because of no genetic variation. That decreases the ability to adapt to changes in the environment. Diseases will be transmitted to the offspring from parent plants rapidly. Also bad traits will be transmitted to the daughter plants.
Desirable traits can be restored due to offspring are genetically identical to parents.
Large food reserves are provided for daughter plants. The process doesnt include external organs or another plant.
leaf blade produces auxins (IAA) p auxins are accumulated in the base of the petiole proots are initiated p roots produce cytokinins (CK) p CK is accumulated in the callus p shoot is stimulated to form
Stem cutting
Process is very much the same as leaf cutting Usually, a short piece of stem (three or four internodes maximum) and together with the apical meristem are cut
leaves send auxins (IAA) down from the blade p stimulate the formation of callus p roots are formed as described in the leave cutting
bud and a small sliver of the wood underneath it are cut from the budstick using an upward and downward slicing motion
vertical cut is made on the stem of the root stock; a perpendicular cut is made at the upper end of the vertical cut
bark is carefully slipped from the stem of the rootstock exposing a "pocket" into which the bud shield can be placed
bark flaps are held tightly against the bud; they are wrapped with a budding rubber, grafting tape or other suitable closure
Layering
A useful method of producing roots on the stem of indoor landscape plants Mainly used for woody plants e.g. magnolia, gardenia, rose
1. wounding the lower surface of a stem and the wound is dusted with rooting hormone
2. moist sphagnum moss or even moist soil is packed around the wound
3. sphagnum/soil is held in place with plastic film 5. rooted branch can then be cut from the donor tree to develop to another individual plant
Applications
Regeneration of plants from organ culture Mass propagation of vegetation for horticultural trade Improve the quality of plants Somatic hybridization (involvement of molecular technique)
CloningCloning a member of a population of genetically identical cells produced from a single cell
The adult sheep is Dolly, the first mammal cloned from an adult cell. The lamb is Dollys offspring, called Bonnie.
PROs
1.Producing a greater understanding of the cause of miscarriages Cloning may lead to a treatment to prevent spontaneous abortions. Also, It might lead to an understanding of the way a morula (mass of cells developed from a blastula) attaches itself to the uterine wall. This might generate new and successful contraceptives.
Cloning can generate genetically modified animal organs that are suitable for transplantation into humans.
3. Preventing child suffering heredity
Cloning could also be used for parents who risk passing a defect to a child. A fertilized ovum could be cloned, and the duplicate tested for disease and disorder.
4.Cure damaged nervous system Damage to the nervous system could be treated through cloning. Damaged adult nerve tissue does not regenerate on its own. However, stem cells might be able to repair the damaged tissue.
CONs
:
1.Reducing genetic variability Producing many clones runs the risk of creating a population that is entirely the same, susceptible to the same diseases 2.Interfering with natural evolution Cloning may cause people to settle for the best existing animals, not allowing for improvement of the species. In this way, cloning could potentially interfere with natural evolution.
Risk of disease transfer between transgenic animals and the animal from which the transgenes were derived.
4.Genetic tailoring of offspring
The ability to clone humans may lead to the genetic tailoring of offspring. This is viewed as inappropriate tampering with "Mother Nature" by many ethicists.
5.Detrimental effect on familial relationships
A child born from an adult DNA cloning of his father could be considered a delayed identical twin of one of his parents.
Sexual Reproduction
less rapid, often involves two parents and produces offspring which are genetically different. The fusion of haploid nuclei is often involved. Apart from purely increasing numbers, reproduction may involve one or more of the following:
(a) a means of increasing genetic variety and therefore helping a species adapt to changing environmental circumstances; (b) the development of resistant stages in a life cycle which are capable of withstanding periods of drought, cold or other adverse conditions.
Dioecious: the male and female sex organs are borne on separate individuals Monoecious: the male and female sex organs are borne on the same individual Cross fertilization: the fusion of gametes from two separate parents Self fertilization: sperms and ova from the same individual fuse
Three sexual life cycles differing in the timing of meiosis and fertilization (syngamy)
Alternation of generations
Reproductive System
I. The Reproductive System produces, stores, nourishes, and releases specialized sex cells known as gametes A. Sexual Developmentboth male and females start out identical. Special hormones then cause production of male or female reproductive organs
Functions of System: produce gametes deliver gametes protect and support gametes
B. Male Reproductive System 1. Testes Testes(male gonads)primary male reproductive organ ScrotumScrotum testes are contained in external sac. Helps to regulate temperature for sperm production scrotum testes
Sperm cells are haploid and are produced in the tubules within the testes.
From the testes they are deposited into the epididymas and then vas deferens to begin swimming and prepare to be deposited into the female.
Human Male Reproductive System testis - paired organs producing sperms; - spermatic cords contain arteries & veins joining the testes; seminiferous tubules p vasa efferentia p epididymis p vas deferens p urethra p penis
prostate gland & Cowper's gland, (1) activating and nourishing the sperms (2) neutralizing the acidity of the vagina
Bulbourethral Gland: lubrication for penis to enter vagina and for sperm to move more easily through the urethra The resulting mixture is called Semen. an ejaculation releases about 3 mL of semen
Testosterone
In addition to stimulating spermatogenesis, testosterone initiates puberty, and it triggers and maintains the sex drive and the formation of secondary sexual characteristics. The hormones are regulated by negative feedback. Testosterone directly inhibits the release of GnRH from hypothalamus and LH from anterior pituitary.
Gametogenesis is the formation of gametes. Spermatogenesis is the production of sperm: Primordial germ cell p MITOSIS p Spermatogonia which grow into Primary spermatocytes p 1st MEIOSIS p Secondary spermatocytes p 2nd MEIOSIS p Spermatids which differentiate into Spermatozoa
Spermatogonia(2n) located near outer wall of seminiferous tubules. Increase in number through repeated mitosis
These spermatogonia grow into larger cells with more cytoplasm called primary spermatocytes.
Each primary spermatocyte carries out the first division to produce two secondary spermatocytes.
Each secondary spermatocyte carries out the second division of meiosis to produce two spermatids
Spermatids become associated with nurse cells, called Sertoli cells which help the spermatids develop into spermatozoa. Cell differentiation
Sperm cells detach from Sertoli cells and are carried out by fluid in the centre of the seminiferous tubules.
Finally we have a bunch of sperm. Millions, in fact, are produced in a male every day. If they are not ejaculated, they are reabsorbed after a couple of weeks by the body and recycled. Sperm are very stream-lined for what they do. They dont have any extraneous gear. No food for the trip and no proteins on their surface. Just DNA with a tail.
Flagellum: propulsion Mitochondria: power Nucleus:DNA Acrosome: a way into the egg (digestive enzymes)
In order to deliver the gametes into the females reproductive tract and to the females gametes, the penis must become erect. Blood flows into the erectile tissue and the build up of blood pressure causes the penis to become rigid. Further stimulation of the nerves on the penis triggers a series of smooth muscle contractions that propel the sperm out of the penis.
Of the millions of sperm cells ejaculated into a female, only a few will survive to meet the egg a couple of days later. This is because the environment into which the sperm will enter is very harsh and the vast majority die: no energy source very acidic The male body provides these things to the sperm cell to aid it on its journey to find the female gamete (the egg): sugar (from seminal vesicle) a basic solution to neutralize the acid (prostate)
In males
Facial hair Deepening of the voice Growth of skeleton Sweat gland development
All female structures are analogous to male structures and must perform the similar functions. Produce gametes Deliver gametes Provide environment for organism to develop if fertilization occurs
Female Reproductive System 1. Ovaries (female gonads)- primary female reproductive organ
a. Produces eggs- process called ovulation (happens about once a month) b. Also produces female hormones- estrogen and progesterone (causes appearance of secondary sex characteristics)
2. Uterus hollow, pear-shaped, muscular Uterusorgan with thick walls in which a fertilized egg develops 3. Vagina lower end of uterus connected to Vaginaoutside of body by muscular tube (also called birth canal)
A female is born with all the egg cells that she will ever have. They are immature primary oocytes and only develop into mature egg cells when the woman enters sexual maturity. From puberty (around age 12) until menopause (around age 50) a female will release 1 egg per 28 days (on average) unless fertilization occurs. Eggs are stored and mature in the ovary. They travel down the fallopian tube (oviduct) to the uterus
Primordial germ cell p MITOSIS p Oogonia which grow into Primary oocytes p 1st MEIOSIS p 1st Polar body (degenerates later) + a Secondary oocyte p 2nd MEIOSIS p 2nd Polar body (degenerates later) + an Ovum
The Menstrual Cycle complex series of periodic events (cycle varies from 20 to 40 days) Controlled by hormones operating on negativefeedback mechanism (from pituitary and ovaries)
Anterior pituitary
Ovaries
Ovaries
Stimulates ovulation, growth of corpus luteum, and secretion of estrogen and progesterone Estrogren Female secondary characteristics, maturation of eggs, growth of uterine lining Progesterone Stimulates growth of uterine lining and placenta formation
LH
The Menstrual Cycle: Hypothalamus releases GnRH GnRh stimulates release of FSH & LH by pituitary FSH & LH stimulate development of follicles Follicles secrete estrogen 2 follicles form per month Increased growth of follicle releases more estrogen Estrogen has 3 effects Promotes further growth of follicle, oocyte Stimulates growth of endometrium in uterus Stimulate increase in FSH & LH
The surge in LH has three effects Trigger meiosis I in oocyte Final explosive growth of follicle & ovulation Turns remnants of follicle into corpus luteum Corpus luteum secretes estrogen & progesterone These inhibit the pituitary & hypothalamus thus stopping FSH & LH This stops formation of further follicles Stimulates further growth of endometrium
.if fertilization occurs or not. If pregnancy does not occur then. the corpus luteum disintegrates after 1 week after ovulation This is because progesterone stops LH production which stimulates corpus luteum growth. Without the corpus luteum, progesterone and estrogen levels drop which causes the endometrium to die. It is shed as menstrual flow that begins about the 27th or 28th day. The reduced level of progesterone means that the hypothalamus and pituitary can start producing GnRH The cycle starts again.
If pregnancy does occur then .. The embryo itself starts secreting a LH like hormone called CG or chorionic gonadotropin that prevents the breakdown of the corpus luteum. Which continues to produce progesterone and estrogen which nourishes the endometrium and the embryo. The embryo releases so much CG that it comes out in the urine and can be used as a pregnancy test.
Birth Control pills contain estrogen and progesterone which suppresses LH which is the hormone that triggers ovulation
Efforts are being made to develop a male pill which will suppress the production of FSH which causes spermatogenesis and continue LH production so that testosterone production. This means a man would be infertile, but not impotent.
Oral Contraception
Combined (estrogen and progestin containing pills)
Failure rate 3/100 women/year
Oral Contraception
Mechanism of Action: Inhibition of ovulation Most contain ethinyl estradiol (2050mcg)
Oral Contraception
Advantages:
Protects against ovarian and endometrial cancers Decreased risk of iron deficiency anemia Decreased incidence in benign breast disease Decreased risk of ovarian cysts Decreases menstrual blood loss
Oral Contraception
Disadvantages: Increases risk of venous thromboembolic disease especially in smokers Risk of worsening uncontrolled hypertension (especially with higher dose estrogen pills) Must be able to remember to take pill daily therefore very high risk for noncompliance No STD protection
Emergency Contraception
3 modalities (administered within 72 hours of unprotected sex)
High dose combined oral contraceptive pills (at least 100mcg of ethinyl estradiol/dose) High dose progestin only pills
Both are 2 doses taken 12 hours apart
Emergency Contraception
Mechanism of Action:
Inhibition of ovulation, interference with fertilization, alteration of endometrial lining NOTE***once implantation takes place emergency contraception has NO effect (ie. Not an abortion pill)
Emergency Contraception
Advantages:
Can prevent pregnancy in assaults and contraceptive failure (broken condom, displaced diaphragm, or missed pills)
75% effective (new data to suggest it is most effective if administered within 24 hours)
Emergency Contraception
Disadvantages:
Not as effective as other modalities 50% associated with nausea 20-25% associated with vomiting No STD protection
Permanent Sterilization
Bilateral Tubal Ligation
PERMANENT Counsel extensively about other long term reversible options Failure rate 4-6/1000
Permanent Sterilization
Vasectomy
PERMANENT Extensive counseling prior to procedure No STD protection Need back up method for several months Failure rate 1-2/1000 Less expensive than a tubal ligation
Barriers et.al.
Diaphragms
Can be used in patients who cant tolerate other forms of BC Requires fitting in doctors office Requires motivated patient Can have a high failure rate because of incorrect or inconsistent use
Intrauterine Device
Name brands: Copper T ($285, good for 10 years) Progestasert ($100, good for one year only) Failure rate: 2/100 women/year Can be used as emergency contraception Mechanism of Action: Inhibition of sperm motility therefore preventing fertilization Previously thought to be inhibition of implantation of fertilized egg
IUD
Advantages:
Easy to use, good for low risk patients No systemic effects May use while breastfeeding
IUD Disadvantages
Risk of uterine perforation Risk of expulsion Can be associated with ascending pelvic infections and infertility in high risk patients Patients may not return for annual pap smears
Enhancing fertility
Causes
Blocked oviducts, low sperm count, blocked sperm ducts Treatment Surgery, hormone therapy
Fertilization to Birth A. Fertilization Fertilization1. arrival of sperm 2. binding 3. acrosome reaction 4. fusion 5. cortical reaction 6. mitosis
Stages in fertilization
1. Sperm deposited into vagina move through uterus into the oviducts (200 to 300 million sperm are deposited) 2. Egg and sperm unite to form zygote
3. Egg moves along oviduct to uterus 4. Fertilized egg implanted in wall of uterus and pregnancy) develops for nine months (called pregnancy
the blastocyst
B. Gastrulation formation of the
trophoblast with the outer layers of cells develop into the embryonic membranes
C. Invagination formation of notochord
Human CG appears to have two distinct roles in maintaining pregnancy. 1. the earliest form of hCG expressed by trophoblasts seems to facilitate embryo implantation. This form of hCG, which is expressed 6-10 days following conception, does not efficiently stimulate ovarian progesterone production. Over the course of the subsequent week, hCG matures into a form that is capable of stimulating ovarian progesterone secretion. 2. hCG stimulates ovarian progesterone secretion in quantities sufficient for the maintenance of pregnancy.
B. Stages of the Embryo and Fetus Embryo1. Embryo during the first two months of pregnancy- unborn child known as an embryo
3. Umbilical cord transports nutrients and cordoxygen from mother to baby. Attached to placenta and embryos navel
Mammalian embryo
Amnion covers the embryo and secretes amniotic fluid. Amniotic fluid acts as a shock absorber, protects the embryo from mechanical shock, prevents the embryo from becoming desiccated and allows it to move. Placenta connect the embryo to the wall of uterus to supply the nutrients and oxygen and remove wastes. Umbilical cord transports blood between the embryo and placenta for exchange of food and wastes.
4. Amniotic sac during third week of sacpregnancy, thin membrane forms around embryo. This sac is filled with clear liquid called amniotic fluid
5. Fetus after first two months of pregnancy, Fetusdeveloping baby called fetus a. All body organs are present b. Develops for total of nine months and is then ready for birth
C. Childbirth Labor1. Labor series of events that expel infant from uterus (muscular contractions of uterus) 2. Amniotic sac usually breaks (breaking water) 3. Contractions cause uterus to widen to allow baby to pass through 4. More powerful contractions push body out through vagina
5. Umbilical cord is cut (scar later forms navel) 6. Contractions expel placenta
Development after Birth and parental care NeonatalA. Neonatal Means new born (first four weeks) Baby totally depends on others to survive. B. Infancy from neonatal to one year. Period of Infancyrapid growth and development. C. Childhood lasts until twelve. Development Childhoodof muscular coordination and mental abilities D. Adolescence begins from age 12-14. AdolescencePuberty1. Puberty time of development when person becomes physically able to reproduce (hormones produced) 2. Time of growth spurt AdulthoodE. Adulthood begins at end of adolescence and extends to old age.
Milk Production
Evolutionary Psychology
Science of Sex Appeal: What Women Find Attractive http://www.youtube.com/watch?v=PoKAa5vEEc&feature=BF&list=PL2DCD5FF59B0BADBF&i ndex=8 Science of Sex Appeal: Falling in Love http://www.youtube.com/watch?v=wK6d7vrN17U&featur e=BF&list=PL2DCD5FF59B0BADBF&index=17
Songbird Infidelity: The development of DNA identification has given scientists potent new tools for discovering the genetic relationships between animal parents and their offspring. In recent years this has led to some eye-opening revelations about monogamy and infidelity in the animal world -particularly in birds, which have traditionally been thought to form monogamous pairs for child-rearing. Because avian offspring require a lot of parental care -- incubating the eggs and feeding the nestlings -- it seemed to both parents' advantage to be hardworking, faithful partners. Scientists using DNA "fingerprinting" have discovered instead that a surprising number of eggs in birds' nests contain another male's genes. Behind the appearance of monogamy, "sexual fidelity is hard to find," as science author Virginia Morell put it. "Social" monogamy -- staying together for the sake of the kids -- is one thing. But among birds, scientists are finding, females are sneaking off with other males whose offspring are then raised by the female and her unknowing partner. Why the deception? A leading theory is that it provides a sort of evolutionary insurance policy for the female bird. By adding some male genes from outside her "family," she's adding some genetic variability that could enable some of her offspring to survive if the environment should change. The female appears to choose "high-quality" males endowed with desirable traits for these "extra pair" matings. For example, female great reed warblers of Scandinavia select males with a larger repertoire