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Fertilization is the process whereby the sperm and the egg (gametes) fuse to form a zygote The zygote is a single celled organism and marks the beginning of a new life The genome of the zygote is derived from both parents
Fertilization contd
It accomplishes two main objectives: 1) Sex-the combining of genes derived from both parents 2) Reproduction-the creation of a new organism Fertilization therefore initiates those reactions in the egg cytoplasm that permit development to proceed among other things
Conception
Generally consists of four major events: 1) Contact and recognition between the egg and the sperm (ensures they are of the same species) 2) Regulation of sperm entry into the egg-only one sperm nucleus can unite with the egg nucleus 3) Fusion of the genetic material of sperm and egg 4) Activation of egg metabolism to start development
Fertilization contd
The egg activates the sperm metabolism essential for fertilization The sperm reciprocates by activating the egg metabolism needed for onset of development
The force for sperm propulsion is provided by a protein called dyenin, attached to the microtubules Dyenin is a an ATPase, an enzyme that can hydrolyze ATP converting the released energy into mechanical energy to propel the sperm The ATP needed to propel the sperm comes from the mitochondria in the middle piece Individuals lacking Dyenin suffer from the genetic syndrome known as Kartagener triad
Sperm maturation
The sperm released during ejaculation can move but cannot bind and fertilize an egg The final stage of sperm maturation is cumulatively referred to as capacitation does not occur until the sperm has been inside the female reproductive tract for a certain period
The egg
All materials necessary for the beginning of growth and development must be stored in the mature egg Unlike the sperm, the egg has retained most of its cytoplasm as it matures The mature egg (Oocyte) conserves most of the material it has and actively acquires more as it reaches meiosis, the stage in which it is fertilized
Egg contd
In some species such as the sea urchins, the egg pronucleus is already haploid at the time of fertilization In some mammals and some worms, the egg nucleus is diploid until the sperm entry and completion of meiosis In these species, the egg meiosis will take place while the sperm nuclear material (sperm pronucleus) is travelling towards what will become the female pronucleus
by five basic steps: Chemoattraction of the sperm to the egg by soluble molecules secreted by the egg Exocytosis of the acrosomal vesicle to release its enzymes Binding of sperm to the extracellular envelope (vitelline layer or zona pellucida) of the egg Passage of sperm through the extracellular matrix Fusion of the egg and sperm
Sperm attraction
Organisms that lay their eggs in water have multiple problems including; How would sperms and eggs meet in a dilute environment? How come sperms do not fertilize eggs of another species? Sperms are attracted to the eggs of their species by chemotaxis-following a gradient of a chemical released by the egg The eggs also regulate the timing of the release of the sperm
Sperm attraction
Sea Urchin spems move as a result of changes in pH In the testes, the pH is about 7.2, but in the water the pH rises to 7.6 This change in pH activates dyenin ATPase Dyenin ATPases hydrolyzes ATP thereby releasing energy The flagella uses this energy to wave and the sperm swims vigorously Sperm direction in echinoderms is provided by a small peptide (14 amino acids) called resact
Acrosome reaction
A second interaction between sperm and egg is the acrosome reaction In most marine invertebrates, the acrosome reaction has two components: 1) Fusion of the acrosomal vesicle with the sperm cell membrane 2) Extension of the acrosomal process The acrosomal reaction in sea urchins is initiated by contact of the sperm with the egg jelly (Fig 7.11)
Species-specific recognition
The first species-specific recognition event is achieved by sperms contact with the egg In sea urchin, another species-specific recognition occurs once the sea urchin sperm penetrates the egg jelly and the acrosomal process of the sperm contacts the surface of the egg (Fig 7.8) The protein mediating this reaction in sea urchins is called bindin
Prevention of polyspermy
The entrance of multiple sperms (polyspermy) in most organisms leads to disastrous consequences In sea urchin fertilization by two sperms leads to a triploid nucleus (3 sets of chromosome) In sea urchins this is achieved in two ways: A fast reaction caused by electric change in the egg cell membrane Slower reaction caused by exocytosis of the cortical granules These two reactions were delineated by the famous African American embryologist, E. E. Just in early 1900s.
Mammalian fertilization
It is difficult to study the interactions between the mammalian sperm and the egg: 1) Fertilization in these species takes place inside the oviducts of the female 2) We do not know the components of the various natural environments inside the female reproductive system that the sperm must encounter on its way to the egg
Mammalian fertilization
It is believed that sperm motility plays a minor role in getting the sperm to the oviduct from the vagina However, sperm motility is required for mouse sperm to travel through the cervical mucosa and for the encounter with the egg Within 30 min of deposition in the vagina, sperms of mice, guinea pigs, cows, and humans, are found in the oviduct (pretty fast for sperm) Sperms appear to be transported to the oviduct by the muscular activity of the uterus