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DEFINITIONS
Molecular biology: The branch
Agriculture: The science, art, or occupation concerned with cultivating land, raising crops, and feeding, breeding, and raising livestock.
INTRODUCTION
Application of molecular biology in agriculture dates way back to at
least 10000years.
Humans have constantly searched for improved varieties of their crop plants and animals. Gene cloning in animals has been on going, knowingly or unknowingly The rate of evolution of new species through natural selection was slow, but now molecular methods like gene cloning have been discovered that can help fasten the process
Plant agriculture
Objectives
GENE CLONING
This is the insertion of a fragment
Gene addition
Gene subtraction
GENE ADDITION
Alters the characteristics of a plant by provision of one or more new genes Applications : Plants that make their own insecticides Herbicide resistant crops
Modified fruit ripening Drought tolerance Improved sweetness Improved flower colour etc
FINDINGS
The factors assessed were damage to foliage and length of tunnels produced by the larvae boring into the plant. Transformed plants showed better results than the non modified. Eg length of tunnels reduced from 40.7cm to 6.3cm
Gene subtraction
The unwanted gene is inactivated Strategies are several but the best is use of antisense RNA This has been used to delay tomato ripening The gene that codes for polygalacturonase enzyme,responsible for ripening is inactivated.
GRAPH
Expression in a normal plant
Comparison: expression in normal and modified plant
III.
IV. Confirming transmission of the transferred gene to the next generation of animals
Limitations
Escape genes Poor produce due to target pests resistance Possible harmful effect of the marker genes used with plant cloning vectors Terminator technology Disruption of the farmland biodiversity ethical issues arise:-moral, religious etc
It is expensive
Un expected yet undetected
Antibody Vaccine
Arntzen group Transgenic potato (Richter et al, 2000) Thomas Jefferson Transgenic lettuce University/Polish Academy of Sciences
Dietary
Phase II
Lactoferrin
Dietary
Gastrointestinal infections
Norwalk virus infection Rabies
Meristem Therapeutics
Arntzen group (Tacket et al, 2000)
Transgenic maize
Transgenic potato
Phase I
Phase I Phase I
References
http://www.ncbi.nlm.nih.gov/pmc/articles/P MC26347/ http://findarticles.com/p/articles/mi_hb1366/ is_1_15/ai_n29297863/ T.A brown () gene cloning and DNA analysis 6th edn