IVY TECH NANOTECH

Article published Aug 7, 2009 South Bend Tribune

Michiana's Nanotech Partnerships Bloom

Ivy Tech students are working in ND research labs this summer.
By MARGARET FOSMOE Tribune Staff Writer SOUTH BEND -- The bacteria glow green under the microscope. "Look at that. They're alive!" says Kathryn Griffin, an Ivy Tech Community College student who is conducting nanotechnology research this summer at the University of Notre Dame. It's part of a growing partnership between Notre Dame, Ivy Tech and Purdue University to train future workers for nanotechnology fields. Nanotechnology involves research and technological development at a scale so tiny it is measured in nanometers -- billionths of a meter. It creates and uses structures that have novel properties because of their size, and it offers the ability to manipulate individual atoms and molecules.

Joshua Shrout, right, a University of Notre Dame professor of civil engineering and biological sciences, discusses the activity of bacteria observed on a monitor in July with three Ivy Tech Community College students who are conducting laboratory research this summer. The Ivy Tech students are, from left, Kathryn Griffin, Amro Mentash and Leigh Weaver. Tribune Photos/JIM RIDER

NOTRE DAME NANOTECH

2008 Semiconductor Research Corporation

Nanoelectronics Research Initiative (NRI)

Purdue

University of Illinois University Michigan

Cornell University Georgia Tech

Penn State

University of Texas Dallas

NOTRE DAME NANOTECH

Ignition Park
Studebaker Corridor Development

Innovation Park
technology to market incubator

COOPERATIVE VENTURES

Research Institutions

Community Colleges

Semiconductor Industries

ROLE OF IVY TECH in the MIND

With Commitment from State and Local Interests

Design and build a clean room facility

- Training mock-up or operational (to be finalized)

Develop an Associate Degree Curriculum

- Operation of clean room environmental equipment - Operation/maintenance of equipment in nanofabrication - Manufacturing technicians

IVY TECH ASSOCIATES DEGREE

In proposal desired launch Fall 2010
First Semester: COMM 101 ENGL 111 IVYT 1XX MATH 136 NANO 101 Fundamentals of Public Speaking English Composition First Year Seminar College Algebra Fundamentals of Nanotechnology I Total: 3 3 1 3 3 13

Fourth Semester: NANO 201 NANO 211 NANO 241 PHYS 101 Nanoelectronics Intro to Materials Characterization Nanoscience Manufacturing Physics I Total: Fifth Semester: ENGL 211 NANO 299 NANO 221 NANO 271 NANO 251 Technical Writing Nanoscience Internship Nanoscience - Specialized Areas Thin film deposition Micro and Nano Fabrication Total: 3 2 3 3 4 15 3 3 3 4 13

Second Semester: CHEM 105 General Chemistry MATH 137 Trigonometry with Analytic Geometry NANO 102 Fundamentals of Nanotechnology II NANO 231 Nanomaterials Total:

5 3 3 3

14

Third Semester (Summer Session): XXXX XXX Hum/Social Sciences Elective 3 XXXX XXX Hum/Soc Sci/Science/Tech Elective 3 to 4 Total: 6 to 7

TOTAL NUMBER OF CREDITS: 61 - 62

PILLARS of NANOTECH

1. Electronics

2. Energy
3. Materials 4. Bio
Dr. Robert Dunn Director of MIND

ATRIBUTES OF BIO-NANOTECH

1. Variety of bio-nanodevices adaptable from living systems 2. Protein-based bio-nanodevices can be engineered at the genetic level 3. Self-assembly
Nature was the first nanotechnologist Dr. Jim Powell, Dean
Ivy Tech Region 2, College of Liberal Arts and Sciences

BIOTECHNOLOGY at IVY TECH

Associates Degree in Biotechnology

One of 6 regional campuses Articulation to local 4 year colleges Region 2 Program fundamentals
General Chemistry
Biology Biotechnology
Including: recombinant DNA, proteins, cell science, microbiology, biomanufacturing, upstream/downstream processing, cGXP, technical writing

DNA ORIGAMI
Two- and three-dimensional shapes created from interlocking or interweaving DNA helixes DNA nanotubes Adherence to semiconductors for DNA chips

Paul Rothemund Cal Tech

Tapping Mode-AFM Imaging of DNA origami in Buffer Solution

Amro Mentash*, Valerie Goss#, and Marya Lieberman#
#The

Community College, Program for Biotechnology, South Bend, IL, 46601 University of Notre Dame, Department of Chemistry & Biochemistry, Notre Dame, IN, 46610

*IvyTech

Question: Can DNA origami deposited on mica be visualized under a buffered solution?

Atomic force microscope (AFM) fitted with a flow-cell

Tapping Mode-AFM Imaging of DNA origami in Buffer Solution

BACTERIAL ATTACHMENT TO
ORGANOSILICON SELF-ASSEMBLED MONOLAYERS

Kathryn Griffith & Leigh Weaver

Ivy Tech Biotechnology Students and

Dr. Marya Lieberman

Department of Chemistry and Biochemistry University of Notre Dame

Notre Dame Nanotechnology Undergraduate Research Fellows (NURF)

George Twaddle
ND RET Fellow
Program Chair for Biotechnology Ivy Tech Community College South Bend

Dr. Joshua Shrout

Department of Engineering and Biological Sciences
University of Notre Dame

BACTERIAL ATTACHMENT TO A SUBSTRATE

Biofilm Formation - communities of one or more microorganisms living

living in a complex web of symbiotic interactions

which start from the attachment of the panktonic or free-living form of a bacteria Factors affecting attachment to a substrate Cell wall structures : pili, carbohydrates, proteins and lipids
Microbiologybytes.com

Cell exudates: proteins, lipids and sugars

Bacterial motility
Terrebonne.online.com

Pseudomonas aeruginosa Widespread: soil, water, skin, man-made environments

Not fastidious eats almost anything Gram-negative lipopolysaccharide covering Other attachment factors: glycolipids and polysaccharides

OPPORTUNISTIC HUMAN PATHOGEN

Infects eyes, ears, mouth, wounds, respiratory tract 1 in 10 hospital acquired infections

Morbidity in people suffering from cystic fibrosis

Credits top to bottom

How can we stop the formation of a P.a. biofilm?

Microlibrary.com Ehagroup.com Am. Soc. Microbiology

STUDYING BACTERIA ATTACHMENT A NANOTECH BOTTOM-UP APPROACH

Our Question: What are the surface characteristics that allow or inhibit the attachment of P. aeruginosa to a substrate

ORGANOSILICON SELF-ASSEMBLED MONOLAYERS (aka SAMs) - chemically-defined surfaces on glass

Sibond.com

ORGANOSILICON CAST OF CHARACTERS

Aminopropyltriethoxysilane (APTES) Trimethylammonium Chloride (TMAC) Octadecyltrichlorosilane (OTS) Phenyltrichlorosilane (PTS) Polyethoxy Glycol (PEG)

HYDROPHOBICITY CHARACTERIZATION Measure of the contact angle (CAM) of the edge of a water bead on our surfaces allowed us to rate them according to their hydrophobicity
160
Advancing Contact Angle

140 120 100 80 60 40 20 0 glass PEG APTES PTS OTS TMAC

Answers.com

MEASURING P.a. ATTACHMENT TO THE MONOLAYERS Procedure 1. Sterile glass surfaces were placed in Copland jars 2. A Green Flourescent Protein (GFP)-tranformed strain of P.a. (JDS 12) was added to a concentration of 10 million cells /ml log-phase cells in buffered salt solution and 10% LB growth media 3. The amount of fluorescent P.a. was determined using an epifluorescent microscope and software that computed percent coverage Some of our representative data:

Ucanhealth.com

Cmbi.bjmu.edu

TIME COURSE OF ATTACHMENT TO NANOSURFACES

Attachment of P.a. to Organosilicon Monolayers
10

Percent Coverage

8 6 4 2 0 1 3 5 Tim e, hours 20 40

Glass PEG APTES PTS OTS TMAC

Surfaces fall into two groups based upon the rate of attachment

EFFECT OF HYDORPHOBICITY ON ATTACHMENT

Relationship of Surface Hydrophobicity t0 Bacterial Attachment at 5 hours
10
Percent coverage

8 6 4 2 0 0 50 100 150
Advancing contact angle

Glass PEG APTES PTS OTS TMAC cumulative Linear (cumulative)

General trend of increased attachment to more hydrophobic surfaceswith APTES and PTS as outliers.

CONCLUSIONS from ATTACHMENT STUDIES

1. Organosilicon SAMs can be used to study the attachment of P.a. to substratum 2. Attachment of P.a. may be driven primarly by hydrophobic interactions 3. Possible role of amino or chloro-groups of atoms improving attachment

4. Possible role of phenyl groups inhibiting attachment

NEXT STEPS FOR NANOTECH @ IVY TECH 1. Work toward Nanotechnology AS Degree Program 2. Hire a nanotechnology program chair to develop courses 3. Fund and outfit a nanotechnology teaching lab capable of performing basic thin film depositions, lithography, metrology with scanning tunneling and atomic force microscopy, etc.

4. Move forward with the University of Notre Dame in design and construction of joint facilities in the Ignition Park.