PRESENTATION

Presented by: Shan-Salah-ud-Din

Rao-Abdul-Rahman 40 37

Ammad-Shahid

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Presented to: Maam Fatima Ijaz Presentation Topic:

Recombinant DNA TECH.

What is recombination?
The exchange of corresponding DNA segments between adjacent chromosomes during the special type of cell division that results in the production of new genetic make up...
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What Is Recombinant DNA?

Recombinant DNA is a form of DNA that does not exist naturally, which is created by combining DNA sequences that would not normally occur together. In terms of genetic modification, recombinant DNA (rDNA) is introduced through the addition of relevant DNA into an existing organismal DNA.

History of recombinant DNA technology

Recombinant DNA technology is one of the recent advances in biotechnology, which was developed by two scientists named Boyer and Cohen in 1973.

 Intentionally modifying genomes of organisms, by natural and artificial processes, for practical Purposes. Three Goals of Recombinant DNA Technology:
1. Eliminate undesirable phenotypic traits in humans, animals, plants and microbes. 2. Combine beneficial traits of two or more organisms to create valuable new organisms. 3. Create organisms that synthesize products of humans need.

DNA recombinant technology

DNA Recombinant Technology

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Basic principle of recombinant DNA technology

The DNA is inserted into another DNA molecule called vector. . The recombinant vector is then introduced into a host cell where it replicates itself, the gene is then produced
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Objective:
Isolation of multiple copies of a desired gene and transfer it to suitable organism to achieve:
Large scale production of protein encoded by the gene. Know the basics of gene structure, function and regulation. Be familiar with the basic methods of molecular genetics. Understand the meaning of DNA sequence and amino acid polymorphisms. The main purpose of rDNA technology is to make large amounts of DNA (gene) &/or its Proteins. (Ex: Insulin).

Steps involve in preparation of Recombinant DNA..

Identification of desired Gene. Isolation of Foreign DNA. Formation of Chimeric DNA. Insertion of Chimeric DNA in host Cell. Selection of recombinant DNA. Gene Cloning.

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Recombinant DNA Process Example:

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Large-scale production of human proteins by genetically engineered bacteria.

Such as : insulin, hormone, Interferon's. Growth

Applications of Recombinant DNA Technology

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Vaccines production
Firstly, the gene in a pathogenic virus that stimulates protective immunity should be identified.
That portion of DNA is then isolated and incorporated into an established harmless virus (e.g. vaccinia virus).
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This new recombinant virus is used as a vaccine. These vaccines are much safer since they do not expose the patients to the actual virus and do not risk to infection.
This method may be useful in vaccines against malaria and many viruses.
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Agricultural applications
Frost resistant crops
Insecticide resistant crops Herbicide resistant crops

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recombinant technology
It is always possible that an antibiotic-resistant plasmid could be accidentally incorporated into a dangerous pathogen with serious medical consequences.

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